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Histone variants in mouse centromeric chromatin

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Summary

Highly purified centromeric heterochromatin was isolated from mouse liver nuclei and the pattern of core histone variants was analyzed. In comparison with total chromatin, the centromeric heterochromatin of young animals was characterized by (1) enrichment in the replication-dependent variants H2A1, H2B2 and H32, (2) reduced amount of the minor variant H2Az and (3) absence of ubiquitinated molecules of H2A. This specific variant pattern changed upon ageing as a result of accumulation of replacement variants so that in adult animals both chromatin preparations exhibited similar pattern for H2A and H2B, while the difference in the profile of H3 variants was preserved.

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Authors and Affiliations

  1. Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

    Valya Russanova, Elena Stephanova, Iliya Pashev & Roumen Tsanev

Authors
  1. Valya Russanova
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  2. Elena Stephanova
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  3. Iliya Pashev
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  4. Roumen Tsanev
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Russanova, V., Stephanova, E., Pashev, I. et al. Histone variants in mouse centromeric chromatin. Mol Cell Biochem 90, 1–7 (1989). https://doi.org/10.1007/BF00225215

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  • DOI: https://doi.org/10.1007/BF00225215

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