Summary
The ciliary crown and the relationship of the ciliary crown to the underlying axoneme were studied by electron microscopy in cilia from hamster and rat trachea and bronchioles, and rabbit trachea. The ciliary crown is a cluster of 4 to 6 fibrils 35 nm long protruding beyond the plasma membrane at the tips of the cilia. The fibrils are well preserved after tannic acidglutaraldehyde-osmium tetroxide fixation and have high contrast with a periodic density of 4.5 nm. They stain relatively weakly with phosphotungstic acid. The surface of the fibrils stains with ruthenium red.
The microtubules of the axoneme end in a plate of electron dense amorphous material. A five layered disc occupies the space between the membrane and the amorphous plate at the tip of the axoneme. The plasma membrane can be dissolved with the detergent triton X-100 without loss of the ciliary crown. This indicates that the ciliary crown is composed of transmembranous filaments which are bound to the disc at the tip of the axoneme.
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References
Anderson, R.G.W.: Isolation of ciliated or unciliated basal bodies from the rabbit oviduct. J. Cell Biol. 60, 393–404 (1974)
Anderson, R.G.W., Hein, C.E.: Distribution of anionic sites on the oviduct ciliary membrane. J. Cell Biol. 72, 482–492 (1977)
Brokaw, C.J.: Computer simulation of flagellar movement. I. Demonstration of stable bend propagation and bend initiation by the sliding filament model. Biophys. J. 12, 564–586 (1972a)
Brokaw, C.J.: Flagellar movement: A sliding filament model. Science 178, 455–462 (1972b)
Cordier, A.C.: Ultrastructure of the cilia of thymic cysts in “nude” mice. Anat. Rec. 181, 227–250 (1975)
Dentler, W.L., Rosenbaum, J.L.: Flagellar elongation and shortening in Chlamydomonas III. Structures attached to the tips of flagellar microtubules and their relationship to the directionality of flagellar microtubule assembly. J. Cell Biol. 74, 747–759 (1977)
Dirksen, E.R., Satir, P.: Ciliary activity in the mouse oviduct as studied by transmission and scanning electron microscopy. Tissue and Cell 4, 389–404 (1972)
Futaesaku, Y., Mizuhira, V.: The new fixation method using tannic acid for electron microscopy and some observations of biological specimens. Proc. 4th Internat'1. Congr. Histochem. Cytochem., pp. 155–156 (1972)
Gilula, N.B., Satir, P.: The ciliary necklace. A ciliary membrane specialization. J. Cell Biol. 53, 494–509 (1972)
Jeffery, P.K., Reid, L.: New observations of rat airway epithelium. A quantitative and electron microscopic study. J. Anat. (Lond.) 120, 295–320 (1975)
Lockwood, W.R.: A reliable and easily sectioned epoxy embedding medium. Anat. Rec. 150, 129 (1964)
Luft, J.H.: Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat. Rec. 171, 369–416 (1971)
Mizuhira, V., Futaesaku, Y.: New fixation for biological membranes using tannic acids. Acta histochem. cytochem. 5, 233–236 (1972)
Pease, D.C.: The ultrastructure of flagellar fibrils. J. Cell Biol. 18, 313–326 (1963)
Plattner, H.: Ciliary granule plaques: Membrane-intercalated particles aggregates associated with Ca2+-binding sites in Paramecium. J. Cell Sci. 18, 257–269 (1975)
Quintarelli, G., Cifonelli, J.A.: On phosphotungstic acid staining II. J. Histochem. Cytochem. 19, 648–653 (1971)
Quintarelli, G., Zito, R.: On phosphotungstic acid staining I. J. Histochem. Cytochem. 19, 641–647 (1971)
Sale, W.S., Satir, P.: Splayed Tetrahymena cilia. A system for analyzing sliding and axonemal spoke arrangements. J. Cell Biol. 71, 589–605 (1976)
Satir, P.: Studies on cilia III. Further studies on the cilium tip and a “sliding filament” model of ciliary motility. J. Cell Biol. 39, 77–94 (1968)
Satir, P.: The present status of the sliding microtubule model of ciliary motion. In: Cilia and Flagella (M.A. Sleigh, eds.), pp. 131–142. London and New York: Academic Press 1974
Sattler, C.A., Staehelin, L.A.: Ciliary membrane differentiations in Tetrahymena pyriformis. J. Cell Biol. 62, 473–490 (1974)
Silverman, L., Glick, D.: The reactivity and staining of tissue proteins with phosphotungstic acid. J. Cell Biol. 40, 761–767 (1969)
Simionescu, N., Simionescu, M.: Galloylglucoses of low molecular weight as mordant in electron microscopy. I. Procedure and evidence for mordanting effect. J. Cell Biol. 70, 608–621 (1976)
Singer, S.J., Nicolson, G.L.: The fluid mosaic model of the structure of cell membranes. Science 175, 720 (1972)
Summers, K.E., Gibbons, I.R.: Adenosine triphosphate-induced sliding of tubules in trypsin treated flagella of sea urchin sperm. Proc. nat. Acad. Sci. (Wash.) 68, 3092–3096 (1971)
Summers, K.E., Gibbons, I.R.: Effects of trypsin on flagellar structures and their relationship to motility. J. Cell Biol. 58, 618–629 (1973)
Wagner, R.C.: The effect of tannic acid on electron images of capillary endothelial cell membranes. J. Ultrastruct. Res. 57, 132–139 (1976)
Warner, F.D., Satir, P.: The structural basis of ciliary bend formation. Radial spoke positional changes accompanying microtuble sliding. J. Cell Biol. 63, 35–63 (1974)
Williams, N.E., Luft, J.H.: Use of a nitrogen mustard derivative in fixation for electron microscopy and observations on the ultrastructure of Tetrahymena. J. Ultrastruct. Res. 25, 271–292 (1968)
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Supported by U.S.P.H.S. Research Grant number HL-12650
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Kuhn, C., Engleman, W. The structure of the tips of mammalian respiratory cilia. Cell Tissue Res. 186, 491–498 (1978). https://doi.org/10.1007/BF00224937
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DOI: https://doi.org/10.1007/BF00224937