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The cells of the rat gastric groove and cardia

An ultrastructural and carbohydrate histochemical study, with special reference to the fibrillovesicular cells

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Summary

The distal wall of the groove between the rat forestomach and glandular stomach is lined with a special type of columnar cells (CCGG) and with fibrillovesicular cells (FVC). The cardiac glands contain cardiac mucous (CMC) and serous cells (CSC). The CCGG contain small mucous granules and special vesicles and tubules. The CMC are filled with large mucous granules and resemble mucous neck cells. The CSC are filled with large proteinaceous granules. The FVC are characterized by long microvilli, apical bundles of microfilaments and a complex “tubulovesicular system”. The pattern of 3H-thymidine incorporation and the presence of immature and transitional forms indicate a possible origin of all the cell types concerned from a common undifferentiated precursor.

The membranes of the tubulovesicular system of FVC as well as the apical cell membrane were reactive to Thiéry's carbohydrate stain. However, lanthanum tracing of the extracellular space and ultrastructural stereoscopy did not reveal a permanent continuity between both membrane systems. The absence of 3H-thymidine label showed that FVC were not proliferative. The structural characteristics of FVC do not account for a secretory, resorptive or receptive function. The special arrangement of microfilaments and the tubulovesicular system suggests an ability to fast changes in surface area.

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Authors and Affiliations

  1. Centre for Electron Microscopy, Medical Faculty, State University of Utrecht, The Netherlands

    W. Wattel M. Sc. & J. J. Geuze

Authors
  1. W. Wattel M. Sc.
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  2. J. J. Geuze
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The authors thank Prof. Dr. M.T. Jansen and Dr. D.G. de Rooij for critical reading of the manuscript, Mr. P.J.M. Zelissen and Mr. D. de Brauw for their valuable contributions in this study, Mr. M.K. Niekerk for his skillful technical assistance, and Mr. R. Geeraths for printing the photographs

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Wattel, W., Geuze, J.J. The cells of the rat gastric groove and cardia. Cell Tissue Res. 186, 375–391 (1978). https://doi.org/10.1007/BF00224928

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