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Synthesis and characterization of a DNA complementary to Xenopus laevis albumin mRNA

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Summary

Albumin complementary DNA (cDNA) was transcribed from purified albumin mRNA from the liver of Xenopus laevis. The resultant cDNA was an almost full length copy as defined by denaturing gel electrophoresis; was hybridized specifically to albumin mRNA with pseudo-first-order reaction kinetics and the mRNA · cDNA hybrid exhibited a sharp melting profile with a Tm of 83 °C. The identity of the cDNA was confirmed by gel electrophoresis following hybridization-arrested translation.

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Authors and Affiliations

  1. The National Hellenic Research Foundation, Biological Research Center, 48, Vass. Constantinou Avenue, T.T. 501/1, Athens, Greece

    Giorgos J. Dimitriadis

  2. Biology Department, School of Science, University of Athens, Panepistimioupolis, Athens, Greece

    Vasso Zongza-Dimitriadis

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  1. Giorgos J. Dimitriadis
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  2. Vasso Zongza-Dimitriadis
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Dimitriadis, G.J., Zongza-Dimitriadis, V. Synthesis and characterization of a DNA complementary to Xenopus laevis albumin mRNA. Mol Cell Biochem 52, 139–144 (1983). https://doi.org/10.1007/BF00224922

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  • DOI: https://doi.org/10.1007/BF00224922

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