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Effects of Ca2+ on the salt-stress response of barley roots as observed by in-vivo 31P-nuclear magnetic resonance and in-vitro analysis

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Abstract

Calcium has been demonstrated to ameliorate the inhibitory effects of high salinity on nutrient transport in plants. Time-course experiments were carried out to study the effect of high Ca2+ (6 mM) supply under saline conditions (100 mM NaCl) on the regulation of intracellular pH in excised barley (Hordeum vulgare L. cv Arivat) roots. In-vivo 31P-nuclear magnetic resonance measurements showed an alkalinization of the vacuolar pH after salt treatment. In the presence of high Ca2+ the extent of salt-induced vacuolar alkalinization was lower. High Ca2+ partially mitigated the salt-induced increase in Na+ content and decrease in K+ content of the root. The pattern of change in the vacuolar pH paralleled that of Na+ accumulation in the root. This correlation is consistent with the involvement of a tonoplast Na+/H+ antiporter in Na+ transport and the role of Ca2+ in Na+ uptake. High salt appeared to decrease the Pi content of the vacuole while high Ca2+ increased this content irrespective of the salt treatment.

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Abbreviations

NMR:

nuclear magnetic resonance

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Authors and Affiliations

  1. Centro de Edafologia y Biologia Aplicada del Segura, CSIC, Apdo. 4195, E-30080, Murcia, Spain

    Vicente Martinez

  2. Department Land, Air and Water Resources, University of California, 95616, Davis, CA, USA

    André Läuchli

Authors
  1. Vicente Martinez
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  2. André Läuchli
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Additional information

We are grateful to Dr. T.W.M. Fan and R.M. Highasi (University of California, Davis, USA) for their valuable help with the NMR experiments. We also thank Dr. J. Norlyn for his technical assistance. V. Martinez was supported by a Fulbright fellowship.

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Martinez, V., Läuchli, A. Effects of Ca2+ on the salt-stress response of barley roots as observed by in-vivo 31P-nuclear magnetic resonance and in-vitro analysis. Planta 190, 519–524 (1993). https://doi.org/10.1007/BF00224791

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  • DOI: https://doi.org/10.1007/BF00224791

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