Skip to main content
SpringerLink
Log in

Glycolytic enzymes in non-photosynthetic plastids of pea (Pisum sativum L.) roots

  • Published:
Planta Aims and scope Submit manuscript

Abstract

The presence of the glycolytic enzymes from hexokinase to pyruvate kinase in plastids of seedling pea (Pisum sativum L.) roots was investigated. The recoveries, latencies and specific activities of each enzyme in different fractions was compared with those of organelle marker enzymes. Tryptic-digestion experiments were performed on each enzyme to determine whether activities were bound within membranes. The results indicate that hexokinase (EC 2.7.1.2) and phosphoglyceromutase (EC 5.4.2.1) are absent from pea root plastids. The possible function of the remaining enzymes is considered.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GADPH:

glyceraldehyde 3-phosphate dehydrogenase

PFK:

phosphofructokinase

PFP:

pyrophosphate: fructose 6-phosphate 1-phosphotransferase

References

  • ap Rees, T. Bryce, J.H., Wilson, P.M., Green, J.H. (1983) Role and location of NAD malic enzyme in thermogenic tissues of Araceae. Arch. Biochem. Biophys. 227, 511–521

    Google Scholar 

  • ap Rees, T., Green, J.H., Wilson, P.M. (1985) Pyrophosphate: fructose 6-phosphate 1-phosphotransferase and glycolysis in non-photosynthetic tissues of higher plants. Biochem. J. 227, 299–304

    Google Scholar 

  • Borchert, S., Grosse, H., Heldt H.W. (1989) Specific transport of inorganic phosphate, glucose 6-phosphate, dihydroxyacetone phosphate and 3-phosphoglycerate into amyloplasts from pea roots. FEBS Lett. 253, 183–186

    Google Scholar 

  • Bowsher, C.G., Hucklesby, D.P., Emes, M.J. (1989) Nitrite reduction and carbohydrate metabolism in plastids purified from roots of Pisum sativum L. Planta 177, 359–366

    Google Scholar 

  • Bowsher, C.G., Rose, J., Boulton, E.L., Nayagam, S., Emes, M.J. (1992) Reductant for glutamate synthase is generated by the oxidative pentose phosphate pathway in pea root plastids. Plant J. 2, 893–898

    Google Scholar 

  • Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of protein utilizing the principal of protein-dye binding. Anal. Biochem. 72, 248–254

    Google Scholar 

  • Dennis, D.T. (1989) Fatty acid biosynthesis in plastids. In: Bio-chemistry and genetics of non-green plastids, pp. 120–129, Boyer, C.D., Shannon, J.C., Hardison, R.C., eds. American Society of Plant Physiologists, Rockville, Md.

    Google Scholar 

  • Dennis, D.T., Blakeley, S., Carlisle, S. (1991) In: Compartmentation of plant metabolism in non-photosynthetic tissues, pp. 77-94, Emes, M.J., ed. Cambridge University Press, UK

  • Denyer, K., Smith, A.M. (1988) The capacity of plastids from developing pea cotyledons to synthesise acetyl CoA. Planta 173, 172–182

    Google Scholar 

  • Dry, I.B., Nash, I.B., Wiskich, J. (1983) The mitochondrial location of hexokinase in pea leaves. Planta 158, 152–156

    Google Scholar 

  • El-Shora, H.M., ap Rees, T. (1991) Intracellular location of NADP-linked malic enzyme in C3 plants. Planta 185, 362–367

    Google Scholar 

  • Emes, M.J., England, S. (1986) Purification of plastids from higher plant roots. Planta 168, 161–166

    CAS  Google Scholar 

  • Emes, M.J., Fowler, M.W. (1979a) The intracellular location of the enzymes of nitrate assimilation in the apices of seedling pea roots. Planta 144, 249–253

    Google Scholar 

  • Emes, M.J., Fowler, M.W. (1979b) Intracellular interactions between the pathways of carbohydrate oxidation and nitrate assimilation in plant roots. Planta 145, 287–292

    Google Scholar 

  • Emes, M.J., Traska, A. (1987) Uptake of inorganic phosphate by plastids purified from the roots of Pisum sativum L. J. Exp. Bot. 38, 1781–1788

    Google Scholar 

  • Entwistle, G., ap Rees, T. (1988) Enzymic capacities of amyloplasts from wheat (Triticum aestivum) endosperm. Biochem. J. 255, 391–396

    Google Scholar 

  • Fletcher, L. (1978) Studies on the heterogeneity of the glycolytic enzymes enolase and phosphofructokinase. Ph.D. thesis, University of Sheffield, UK

    Google Scholar 

  • Fowler, M.W., Clifton, A. (1975) Hexokinase activity in cultured sycamore cells. New Phytol. 75, 533–538

    Article  Google Scholar 

  • Frehner, M., Pozueta-Romera, J., Akazawa, T. (1990) Enzyme sets of glycolysis, gluconeogenesis and oxidative pentose phosphate pathway, are not complete in non-green highly purified amyloplasts of sycamore (Acer pseudoplantus L.) cell suspension cultures. Plant Physiol. 94, 538–544

    Google Scholar 

  • Ireland, R.J., De Luca, V., Dennis, D.T. (1980) Characterisation and kinetics of pyruvate kinase from developing castor oil seed endosperm. Plant Physiol. 65, 1188–1193

    Google Scholar 

  • Journet, E.P., Douce, R. (1985) Enzymic capacities of purified cauliflower bud plastids for lipid synthesis and carbohydrate metabolism. Plant Physiol. 79, 458–467

    Google Scholar 

  • Joyard, J., Billecocq, A., Bartlett, S.G., Block, M.A., Chua, N.-H., Douce, R. (1983) Localisation of polypeptides to the cytosolic side of the outer envelope membrane of spinach chloroplasts. J. Biol. Chem. 258, 10000–10006

    Google Scholar 

  • Kleppinger-Sparace, K.F., Stahl, R.J., Sparace, S.A. (1992) Energy requirements for fatty acid and glycerolipid biosynthesis from acetate by pea root plastids. Plant Physiol. 98, 723–727

    Google Scholar 

  • Losada, M., Paneque, A. (1971) Nitrite reductase. Methods Enzymol. 23, 487–491

    Google Scholar 

  • MacDonald, F., ap Rees T. (1983) Enzymic properties of amyloplasts from suspension cultures of soybean. Biochim. Biophys. Acta 755, 81–89

    Google Scholar 

  • Miernyk, J.A., Dennis, D.T. (1984) Enolase isozymes from Ricinus communis. Partial purification and characterisation of the isozymes. Arch. Biochem. Biophys. 233, 643–651

    Google Scholar 

  • Miernyk, J.A., Dennis, D.T. (1992) A developmental analysis of the enolase isozymes from Ricinus communis. Plant Physiol. 99, 748–750

    Google Scholar 

  • Plaxton, W.C. (1988) Purification of pyruvate kinase from germinating castor bean endosperm. Plant Physiol. 86, 1064–1069

    Google Scholar 

  • Plaxton, W.C. (1990) Glycolysis. In: Methods in plant biochemistry, vol. 3: Enzymes of primary metabolism, pp. 145–147, Lea, P.J., ed. Plenum, London New York San Francisco

    Google Scholar 

  • Randall, D.D., Miernyk, J.A., Fang, T.K., Budde, R.J.A., Schulter, K.A. (1989) Regulation of the pyruvate dehydrogenase complexes in plants. Ann. N.Y. Acad. Sci. 573, 192–205

    Google Scholar 

  • Rutter, W.J., Richards, O.C., Woodfin, B.M. (1961) Comparative studies of liver and muscle aldolase. Effect of carboxypeptidase on catalytic activity. J. Biol. Chem. 236, 3193

    Google Scholar 

  • Schnarrenberger, C. (1990) Characterisation and compartmentation, in green leaves, of hexokinases with different specificities for glucose, fructose and mannose and for nucleoside triphosphates. Planta 181, 249–255

    Google Scholar 

  • Scopes, K.R. (1975) 3-phosphoglycerate kinase of skeletal muscle. Methods Enzymol. 42, 127–134

    Google Scholar 

  • Scott, K.J., Craigie, J.S., Smillie, R.M. (1964) Pathways of respiration in plant tumours. Plant Physiol. 39, 323–327

    Google Scholar 

  • Simcox, P.D., Reid, E.E., Canvin, D.T., Dennis, D.T. (1977) Enzymes of the glycolytic and pentose phosphate pathways in proplastids from the developing endosperm of Ricinus communis. Plant Physiol. 59, 1228–1232

    Google Scholar 

  • Sparace, S.A., Menassa, R., Kleppinger-Sparace, K.F. (1988) A preliminary analysis of fatty acid synthesis in pea roots. Plant Physiol. 87, 134–137

    Google Scholar 

  • Stitt, M., ap Rees, T. (1979) Capacities of pea chloroplasts to catalyse the oxidative pentose phosphate pathway and glycolysis. Phytochemistry 18, 1905–1911

    Google Scholar 

  • Tyson, R.H., ap Rees, T. (1988) Starch synthesis by isolated amyloplasts from wheat endosperm. Planta 175, 33–38

    Google Scholar 

  • Van Ginkel, G., Brown, J.S. (1978) Endogenous catalase and superoxide dismutase activities in photosynthetic membranes. FEBS Lett. 94, 284–28

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Metabolism Research Unit, Williamson Building, Department of Cell and Structural Biology, University of Manchester, M13 9PL, Manchester, UK

    Bronwen A. Trimming & Michael J. Emes

Authors
  1. Bronwen A. Trimming
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael J. Emes
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Bronwen A. Trimming gratefully acknowledges the award of a studentship from the Science and Engineering Research Council

Rights and permissions

Reprints and permissions

About this article

Cite this article

Trimming, B.A., Emes, M.J. Glycolytic enzymes in non-photosynthetic plastids of pea (Pisum sativum L.) roots. Planta 190, 439–445 (1993). https://doi.org/10.1007/BF00224781

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00224781

Key words

Search

Navigation