Summary
The yeast Cryptococcus neoformans may develop under certain conditions a large polysaccharide capsule 50–100 μM in diameter and therefore cannot be phagocytosed by either polymorphonuclear cells (PMN's) or mononuclear phagocytes (MN's). The cellular defense mechanism — in various animals — against the yeast is composed by formation of ringlike structure of PMN's or MN's cells which surround the C. neoformans. Ring structures develop either in vivo or in vitro in tissue culture; destruction of the yeast occurs within 36–72 hours.
Several hydrolases, such as acid phosphatase, β-glucuronidase and non-specific esterase were found to be released from the phagocytic cells into the enclosed yeast. Considerable reduction of NBT used as a marker for oxidative activity was observed in MN rings at contact regions of the MN cells and the yeast. Electron microscopic studies indicate that the phagocytic cells in the ring structure have many pseudopodes penetrating into the polysaccharide capsule of the yeast. Disintegration of the capsule was observed as well as phagocytosis of its material. A possible analogy between normal phagocytosis of small-sized bodies and the ring structure obtained when large bodies are involved is discussed.
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Aronson, M., Kletter, J.: Aspects of the defense against a large-sized parasite, the yeast Cryptococcus neoformans. In: Dynamic aspects of host-parasite interrelationship, edit. by Weiss, D., and Zuckerman, A., vol. I, p. 85–117. New York: Academic Press 1973
Bainton, D. F., Farquhar, M. G.: Differences in enzyme content of azurophil and specific granules of polymorphonuclear leucocytes. II. Cytochemistry and electron microscopy of bone marrow cells. J. Cell Biol. 39, 299–317 (1968)
Bitensky, C.: Modification to the Gomori acid phosphatase technique for controlled-temperature frozen sections. Quart. J. micr. Sci. 104, 193–204 (1963)
Burstone, M. S.: The relationship between fixation and technique for the histochemical localization of hydrolytic enzymes. J. Histochem. Cytochem. 6, 322–339 (1958)
Cohn, Z. A., Hirsch, H. G., Wiener, E.: Lysosomes and endocytosis. In: Ciba Foundation Symposium on Lysosomes, edit. by de Reuck, A.V.S., and Camerson, M. P., p. 126–142. London: J. & A. Churchill Ltd. 1963
Edwards, M. R., Gordon, M. A., Lepa, E. W., Ghiorse, W. C.: Micromorphology of Cryptococcus neoformans. J. Bact. 94, 766–777 (1967)
Gadebusch, H. H., Johnson, A. G.: Natural host resistance to infection with Cryptococcus neoformans. IV. The effect of some cationic proteins on the experimental disease. J. infect. Dis. 116, 551–565 (1966)
Hayashi, M., Nakajima, Y., Fishman, W. H.: The cytologic demonstration of β-glucuronide and hexazonium pararosanilin, a preliminary report. J. Histochem. Cytochem. 12, 3–4 (1964)
Henson, P. M.: The immunologic release of constituents from neutrophil leucocytes. II. Mechanism of release during phagocytosis and adherence to nonphagocytosable surfaces. J. Immunol. 107, 1547–1557 (1971)
Jenkin, C. R.: The effect of opsonin on the intracellular survival of bacteria. Brit. J. exp. Path. 44, 47–57 (1963)
Johnston, R. R., Klemperer, M. R., Alper, C. A., Rosen, F. S.: The enhancement of bacterial phagocytosis by serum J. exp. Med. 129, 1275–1290 (1969)
Kalina, M., Kletter, J., Sahar, A., Aronson, M.: Acid phosphatase release from intact phagocytic cells surrounding a large-sized parasite. Proc. Soc. exp. Biol. (N.Y.) 136, 407–410 (1971)
Littmann, M. L., Tsubura, E.: Effect of degree of encapsulation upon virulence of Cryptococcus neoformans. Proc. Soc. exp. Biol. (N.Y.) 101, 733–739 (1959)
McRipley, R. J., Sbarra, A. J.: Role of the phagocyte in host-parasite interactions. XII. Hydrogen peroxide-myeloperoxidase bactericidal system in the phagocyte. J. Bact. 94, 1425–1430 (1967)
Oren, R., Farnham, A. E., Saito, K., Milofsky, E., Karnovsky, M. L.: Metabolic patterns in three types of phagocytizing cells. J. Cell Biol. 17, 487–501 (1963)
Rozenstain, L., Leibovich, M., Shoham, D., Epstein, J.: The esterase activity in megaloblasts and normal haemopoietic cells. Brit. J. Haemat. 14, 605–610 (1968)
Spicer, S. S., Hardin, J. H.: Ultrastructure, cytochemistry and function of neutrophil leukocyte granules. A review. Lab. Invest. 20, 488–497 (1969)
Wright, D. G., Malawista, S. E.: The mobilization and extracellular release of granular enzymes from human leukocytes during phagocytosis. J. Cell Biol. 53, 788–797 (1972)
Zeya, H. I., Spitznagel, J. K.: Cationic protein-bearing granules of polymorphonuclear leukocytes: separation from enzyme-rich granules. Science 163, 1069–1071 (1969)
Zurier, R. B., Hoffstein, S., Weissmann, G.: Mechanisms of lysosomal enzyme release from human leukocytes. I. Effect of cyclic nucleotides and colchicine. J. Cell Biol. 58, 27–41 (1973)
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Kalina, M., Kletter, Y. & Aronson, M. The interaction of phagocytes and the large-sized parasite Cryptococcus neoformans: Cytochemical and ultrastructural study. Cell Tissue Res. 152, 165–174 (1974). https://doi.org/10.1007/BF00224692
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DOI: https://doi.org/10.1007/BF00224692