Summary
Four types of acidophilic granular cells, in addition to B-cells, are identified in the islet organ of anadromous specimens of two subspecies of Petromyzon marinus by light and electron microscopy. Three of these acidophils (PI, pII and PIV-cells) occur in both the cranial and hepatic islets while a fourth type (PIII-cell) has only been found in the hepatic islet of some animals. The granules of the PI-cells stain with ponceau de xylidine, give a distinct tryptophan reaction and in ultrastructural examination show large, dense granules. The PII-cells contain unusual crystals and appear to be a non-secretory stage of the PI. The PIII-cells stain deep-red with acid fuchsin. They contain very large, dense granules and some lysosomes. PIV-cells stain selectively with phosphotungstic acid-hematoxylin and ultrastructurally, contain small, more or less dense granules. It appears that PI- and PIV-cells develop directly from B-cells, while the PIII-cells derive from PI-cells. Despite their direct or indirect origin from B-cells, the pI-, PIII- and PIV-cells show characteristic features of functionally independent endocrine cells. Petromyzon marinus may be an ideal model for the understanding of phylogenetic and pathological interrelationships between islet and gastrointestinal hormones. It is clear that the interpretation of the islet organ of the cyclostomes, which has been generally considered a source of insulin only, requires a revaluation.
This study was supported by funds from PHS grant RR-5414 to A. Epple
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We gratefully acknowledge the help of Mr. T. Chiotti (New York State Department of Environmental Conservation) in obtaining the specimens of Petromyzon m. dorsatus, and the donation of the specimens of Petromyzon m. marinus by Mr. W.F. Brinkman, Philadelphia. The manuscript preparation by Shirley W. Nett is also appreciated
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Brinn, J.E., Epple, A. New types of islet cells in a cyclostome, Petromyzon marinus L.. Cell Tissue Res. 171, 317–329 (1976). https://doi.org/10.1007/BF00224657
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DOI: https://doi.org/10.1007/BF00224657