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Cytochemical demonstration of catecholamines in cells of the carotid body in primary tissue culture

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Summary

Cells of the glomus carotioum of cat and rabbit were cultured in primary tissue culture. Under the phase-contrast microscope three types of cells were found, epitheloid cells, spindle-shaped ones like Schwann cells and fibroblasts. After treatment with formaldehyde gas the epitheloid cells showed the characteristic catecholamine fluorescence. Their origin from the glomoids and their behaviour in culture which was similar to that of sympathetic neurons are discussed.

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References

  • Biscoe, T. J.: Carotid Body: Structure and function. Physiol. Rev. 51, 437–495 (1971)

    Google Scholar 

  • Böck, P., Stockinger, L., Vyslonzil, E.: Die Feinstruktur des Glomus carotioum beim Menschen. Z. Zellforsch. 105, 543–568 (1970)

    Google Scholar 

  • Chiocchio, S. R., King, M. P., Carballo, L., Angelakos, E. T.: Monoamines in the carotid body cells of the cat. J. Histochem. Cytochem. 19, 621–626 (1971)

    Google Scholar 

  • Corrodi, H., Hillarp, N. A., Jonsson, G.: Fluorescence methods for the histochemical demonstration of monoamines. III. Sodium borohydride reduction of the fluorescent compounds as a specificity test. J. Histochem. Cytochem. 12, 582 (1964)

    Google Scholar 

  • Dearnaley, D. P., Fillenz, M., Woods, R. I.: The identification of dopamine in the rabbit's carotid body. Proc. roy. Soc. B 170, 195–203 (1968)

    Google Scholar 

  • Eränkö, O., Eränkö, L., Hill, C. E., Burnstock, G.: Hydrocortisone-induced increase in the number of small intensely fluorescent cells and their histochemically demonstrable catecholamine content in cultures of sympathetic ganglia of the newborn rat. Histochem. J. 4, 49–58 (1972)

    Google Scholar 

  • Euler v., U. S.: Synthesis, uptake and storage of catecholamines in adrenergic nerves, the effect of drugs. In: Blaschko, H., Muscholl, E., Catecholamines, pp. 186–230. Berlin-Heidelberg-New York: Springer 1972

    Google Scholar 

  • Falck, B., Hillarp, N. A., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354 (1962)

    Google Scholar 

  • Helpap, B., Hempel, K.: Über den Katecholamin-Stoffwechsel des Carotiskörperchens der Ratte. Virchows Arch. Abt. B 3, 270–281 (1969)

    Google Scholar 

  • Kirshner, N., Viveros, O. H.: The secretory cycle in the adrenal medulla. Pharmacol. Rev. 24, 385–398 (1972)

    Google Scholar 

  • Kock, L. L. de: The intra-glomerular tissue of the carotid body. Acta anat. (Basel) 21, 101–116 (1954)

    Google Scholar 

  • Korkala, O., Hervonen, A.: Origin and development of the catecholamine-storing cells of the human fetal carotid body. Histochemie 37, 287–297 (1973)

    Google Scholar 

  • LeDouarin, N., Le Lièvre, D., Fontaine, J.: Embryologie Expérimentale.—Recherches expérimentales sur l'origine embryologique du corps carotidien chez les oiseaux. C. R. Acad. Sci. (Paris) 275, 583–586 (1972)

    Google Scholar 

  • Lever, J. D., Lewis, P. R., Boyd, J. D.: Observations on the fine structure and histochemistry of the carotid body in the cat and rabbit. J. Anat. (Lond.) 93, 478–490 (1959)

    Google Scholar 

  • Mains, R. E., Patterson, P. H.: Primary cultures of dissociated sympathetic neurons. II. Initial studies on catecholamine metabolism. J. Cell Biol. 59, 346–360 (1973a)

    Google Scholar 

  • Mains, R. E., Patterson, P. H.: Primary cultures of dissociated sympathetic neurons. III. Changes in metabolism with age in culture. J. Cell Biol. 59, 361–366 (1973b)

    Google Scholar 

  • Möllmann, H., Knoche, H., Niemeyer, D. H., Alfes, H., Kienecker, E. W., Decker, S.: Experimenteller Beitrag zur Kenntnis der biogenen Amine im Glomus caroticum des Kaninchens. Elektronen und fluoreszenzmikroskopische Untersuchungen nach Reserpinund PCPA-Applikation. Z. Zellforsch. 124, 238–246 (1972)

    Google Scholar 

  • Murray, M. R.: Nervous tissues in vitro. In: Willmer, E. N.: Cells and tissues in culture, vol. II, pp. 373–455. London-New York: Academic Press 1965

    Google Scholar 

  • Pietruschka, F., Acker, H., Gattermann, S., Seidl, E., Lübbers, D. W.: Cells of the carotid body in primary tissue cultures. Arzneimittel-Forsch. (Drug Res.) 23, 1607–1614 (1973)

    Google Scholar 

  • Willmer, E. N.: Cells and tissue in culture, vol. I, pp. 19–86. London-New York: Academic Press 1965

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Systemphysiologie Dortmund, Germany

    Fricke Pietruschka

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  1. Fricke Pietruschka
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The author is indebted to Mrs. S. Gattermann for her skilful technical assistance.

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Pietruschka, F. Cytochemical demonstration of catecholamines in cells of the carotid body in primary tissue culture. Cell Tissue Res. 151, 317–321 (1974). https://doi.org/10.1007/BF00224542

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  • DOI: https://doi.org/10.1007/BF00224542

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