Theoretical and Applied Genetics

, Volume 89, Issue 7–8, pp 831–837 | Cite as

A linkage map of diploid Avena based on RFLP loci and a locus conferring resistance to nine isolates of Puccinia coronata var. ‘avenae

  • P. J. Rayapati
  • J. W. Gregory
  • M. Lee
  • R. P. Wise


An F2 oat population was produced by crossing the diploid (n=7) species Avena strigosa (CI 3815) with A. wiestii (CI 1994), resistant and susceptible, respectively, to 40 isolates of Puccinia coronata, the causal agent of crown rust. Eighty-eight F2 individuals were used to construct an RFLP linkage map representing the A genome of cultivated hexaploid oat. Two hundred and eight RFLP loci have been placed into 10 linkage groups. This map covers 2416 cM, with an average of 12 cM between RFLP loci. Eighty-eight F3 lines, derived from F2 individuals used to construct the map, were screened for resistance to 9 isolates of P. coronata. One locus, Pca, was found to confer a dominant resistance phenotype to isolates 203, 258, 263, 264B, 290, 298, 325A, and 345. Pca also conferred resistance to isolate 276; however, an unlinked second gene may also be involved.

Key words

Genetics Disease resistance Monocots 


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  1. Burr B, Burr FA, Thompson KH, Albertsen MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526Google Scholar
  2. Doebley J, Stec A (1991) Genetic analysis of the morphological differences between maize and teosinte. Genetics 129:285–295Google Scholar
  3. Finkner VC (1954) Genetic factors governing resistance and susceptibility of oats to Puccinia coronata Corda var. ‘avenae’ F. and L., Race 57. Iowa Agric Exp St Res Bull 411:1040–1063Google Scholar
  4. Flor HH (1955) Host-parasite interaction in flax rust — its genetics and other implications. Phytopathology 45:680–685Google Scholar
  5. Forsberg RA, Shands HL (1969) Breeding behavior of 6X-amphiploid x Avena sativa F1 hybrids. Crop Sci 9:67–69Google Scholar
  6. Gebhardt C, Ritter E, Barone A, Debner T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57Google Scholar
  7. Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374Google Scholar
  8. Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrman RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256Google Scholar
  9. Hart GE, Gale MD (1990) Biochemical/molecular loci of hexaploid wheat (Triticum aestivum, 2n=42, genomes AABBDD). In: O'Brien SJ (ed) Genetic maps, 5th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 6.26–6.38Google Scholar
  10. Hulbert SH, Bennetzen JL (1991) Recombination at the Rp1 locus of maize. Mol Gen Genet 226:377–382Google Scholar
  11. Jellen EN, Rooney WL, Phillips RL (1993) Characterization of the hexaploid oat Avena byzantina cv ‘Kanota’ monosomic series using C-banding and RFLPs. Genome 36:962–970Google Scholar
  12. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175Google Scholar
  13. Ladizinsky G (1989) In: Ladizinsky G (ed) IBPGR report of a working group on Avena — biological species and wild genetic resources in Avena. IBPGR Press, Rome, pp 19–32Google Scholar
  14. Lagudah ES, Appels R, Brown AHD, McNeil D (1991) The molecular genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat. Genome 34:375–386Google Scholar
  15. Lander ES, Botstein D (1987) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199Google Scholar
  16. Marshall HG, Myers WM (1961) A cytogenetic study of certain interspecific Avena hybrids and the inheritance of resistance in diploid and tetraploid varieties to races of crown rust. Crop Sci 1:29–34Google Scholar
  17. Marshall HG, Shaner GE (1992) Genetics and inheritance in oat. In: Marshall HG, Sorrells ME (eds) Oat science and technology. Crop Science Society of America Pub, Madison, Wis., pp 510–573Google Scholar
  18. Mather K (1951) The measurement of linkage. John Wiley and Sons, New YorkGoogle Scholar
  19. McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829Google Scholar
  20. McVey DV, Leonard KJ (1990) Resistance to wheat stem rust in spring spelts. Plant Dis 74:966–969Google Scholar
  21. Melz G, Schlegel R, Thiele V (1992) Genetic linkage map of rye (Secale cereale L.). Theor Appl Genet 85:33–45Google Scholar
  22. Milne DL, Mcintosh RA (1990) Triticum aestivum (common wheat). In: O'Brien SJ (ed) Genetic maps, 5th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 6.16–6.27Google Scholar
  23. Nof E, Dinoor A (1981) The manifestation of gene-for-gene relationships in oats and crown rust. Phytoparasitica 9:240Google Scholar
  24. O'Donoughue LS, Wang Z, Roder M, Kneen B, Leggett M, Sorrells ME, Tanksley SD (1992) An RFLP-based linkage map of oats based on a cross between two diploid taxa (Avena atlantica x A. hirtula). Genome 35:765–771Google Scholar
  25. Paran IR, Kesseli R, Michelmore RW (1991) Identification of restriction-fragment-length-polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenic lines. Genome 34:1021–1027Google Scholar
  26. Rick CM (1969) Controlled introgression of chromosomes of Solanum pennellii into Lycopersicon esculentum: segregation and recombination. Genetics 62:753–768Google Scholar
  27. Rothman PG (1984) Registration of four stem rust and crown rust resistant oat germ plasm lines. Crop Sci 24:1217–1218Google Scholar
  28. Sadanaga K, Simons MD (1960) Transfer of crown rust resistance of diploid and tetraploid species to hexaploid oats. Agron J 52:282–288Google Scholar
  29. Simons MD, Sadanaga K, Murphy HC (1959) Inheritance of resistance of strains of diploid and tetraploid species of oats to races of the crown rust fungus. Phytopathology 49:257–259Google Scholar
  30. Simons MD, Martens JW, McKenzie RIH, Nishiyama I, Sadanaga K, Sebesta J, Thomas H (1978) Oats: a standardized system of nomenclature for genes and chromosomes and catalog of genes governing characters. Agriculture Handbook Number 509, USDA, Washington, D.C.Google Scholar
  31. Singh NK, Shepherd KW, Mcintosh RA (1990) Linkage mapping of genes for resistance to leaf, stem and stripe rusts and omega-secalins on the short arm of rye chromosome 1R. Theor Appl Genet 80:609–616Google Scholar
  32. Sogaard B, von Wettstein-Knowles P (1987) Barley: genes and chromosomes. Carlsberg Res Commun 52:123–196Google Scholar
  33. Tanksley SD, Ganal MW, Prince JP, Vicente MC de, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messenger R, Miller L, Paterson A, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1991) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1119–1130Google Scholar
  34. Vallejos CE, Sakiyama NS, Chase CD (1992) A molecular markerbased linkage map of Phaseolus vulgaris L. Genetics 131:733–740Google Scholar
  35. Veldboom LR, Lee M, Woodman WL (1994) Molecular marker-facilitated studies of morphological traits in maize. I. Linkage analysis and determination of QTL for morphological traits. Theor Appl Genet 88:7–16Google Scholar
  36. Wang ML, Atkinson MD, Chinoy CN, Devos KM, Gale MD (1992) Comparative RFLP-based genetic maps of barley chromosome 5 (1H) and rye chromosome 1R. Theor Appl Genet 84:339–344Google Scholar
  37. Werner JE, Endo TR, Gill BS (1992) Toward a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311Google Scholar
  38. Whitkus R, Doebley J, Lee M (1992) Coparative genome mapping of sorghum and maize. Genetics 132:1119–1130Google Scholar
  39. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful genetic markers. Nucleic Acids Res 18:6531–6535Google Scholar
  40. Wise RP, Ellingboe AH (1985) Fine structure and instability of the Ml-a locus in barley. Genetics 111:113–130Google Scholar
  41. Wise RP, Gobelman-Werner KS (1993) Resistance to oat crown rust in diploid and hexaploid Avena. Plant Dis 77:355–358Google Scholar
  42. Zamir D, Tadmor Y (1986) Unequal segregation of nuclear genes in plants. Bot Gaz 147:355–358Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • P. J. Rayapati
    • 1
  • J. W. Gregory
    • 2
  • M. Lee
    • 1
  • R. P. Wise
    • 2
    • 3
  1. 1.Department of AgronomyIowa State UniversityAmesUSA
  2. 2.Department of Plant PathologyIowa State UniversityAmesUSA
  3. 3.Field Crops Research USDA-ARSIowa State UniversityAmesUSA

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