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Detection of endo-xylanase activities in electrophoretic gels with congo red staining

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Biotechnology Techniques

Summary

A modification of the Congo Red zymographic technique is used to analyse multiple xylanase activities of Bacillus sp. MIR-32 after PAGE and SDS-PAGE. Xylanase activities are associated with at least two major proteins of 18.4 and 19.6 kDa.

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References

  • Balakrishnan, H., Dutta-Choudhury, M., Srinivasan, M.C. and Rele, M.V. (1992). World J. Microbiol. Biotechnol. 8, 627–631.

    Google Scholar 

  • Bielicki, S. and Galas, E. (1991) Crit. Rev Biotechnol. 10, 275–304.

    Google Scholar 

  • Coughlan, M.P. and Hazlewood, G.P. (1993) Biotechnol. Appl. Biochem. 17, 259–289.

    Google Scholar 

  • Davis, B.J. (1964) Ann. New York Acad. Sci. 121, 404–427.

    Google Scholar 

  • Esteban, R., Villanueva, J.R. and Villa, T.G. (1982) Can. J. Microbiol. 28, 733–739.

    Google Scholar 

  • He, L., Bickrstaff G.F., Paterson A. and Buswell J.A. (1993) Enz. Microb. Technol. 15, 13–18.

    Google Scholar 

  • Kluepfel, D., Daigneault, N., Morosoli, R. and Sharek F. (1992) Appl. Microbiol. Biotechnol. 36, 626–631.

    Google Scholar 

  • Laemmli, U.K. (1970) Nature (London) 227, 680–685.

    Google Scholar 

  • McKenzie, C.R. and Williams, R.E. (1984). Can. J. Microbiol. 30, 1522–1525.

    Google Scholar 

  • Morag, E., Bayer, E.A. and Lamed, R. (1990) J. Bacteriol. 172, 6089–6105.

    Google Scholar 

  • Nakamura, S., Wakabayashi, K., Nakai R., Aono, R. and Horikoshi, K. (1993). Appl. Environm. Microbiol. 59, 2311–2316.

    Google Scholar 

  • Paul, J. and Varma, A.K. (1992). Biotechnol. Lett. 14, 207–212.

    Google Scholar 

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Authors and Affiliations

  1. Cátedra de Microbiología Superior, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán - PROIMI, Avda. Belgrano y Caseros, 4000, Tucumán, Argentina

    Javier D. Breccia, Mario D. Baigorf, Guillermo R. Castro & Faustino Siñeriz

Authors
  1. Javier D. Breccia
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  2. Mario D. Baigorf
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  3. Guillermo R. Castro
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  4. Faustino Siñeriz
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Breccia, J.D., Baigorf, M.D., Castro, G.R. et al. Detection of endo-xylanase activities in electrophoretic gels with congo red staining. Biotechnol Tech 9, 145–148 (1995). https://doi.org/10.1007/BF00224415

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  • DOI: https://doi.org/10.1007/BF00224415

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