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Enzymatic method to determine biomass in skim milk whey permeate medium

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Biotechnology Techniques

Summary

In this study, a new technique for biomass determination was developed. Proteases were used to separate cells from the culture broth in skim milk whey permeate medium and the solubilization of casein was achieved 99% by elastase, 95% by pepsin and 90% by trypsin. Elastase was found to be efficient and reproducible for determining biomass in skim milk whey permeate medium.

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References

  • Ezzat, N., M. El Soda, C. Bouillanne, C. Zevaco and P. Blanchard (1985). Milchwissenschaft 40, 140–143.

    Google Scholar 

  • Kanasaki, M., S. Breheny, A.J. Hiller and G.R. Jago (1975). Aust. J. Dairy Technol. 30, 142–144.

    Google Scholar 

  • Legowo, A.M., T. Imade and S. Hayakawa (1993). Food Resh. Intl. 20, 103–108.

    Google Scholar 

  • Lopóez-Fandiño, R. and Y. Ardö. (1991). J. Dairy Res. 58, 469–475.

    Google Scholar 

  • Neviani, E. and G. Mucchetti (1984). Microbiologie-Aliment-Nutrition 2, 95–97.

    Google Scholar 

  • Singh, H. and P.F. Fox. (1989). In “Chapter 4. Heat induced changes in casein” Ed. P.F. Fox. Bull. Int. Dairy Federation No. 238, 24–30.

  • Stryer, L. 1981. Biochemistry, 2nd eds. W.H. Freeman and Company, New York. p. 167.

    Google Scholar 

  • Trépanier, G., M. El Abboudi, B.H. Lee and R.E. Simard. 1992. J. Food Sci. 57, 345–349.

    Google Scholar 

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Authors and Affiliations

  1. Research and Development, Ault Foods Limited, 75A Bathurst Street, N6B 1N8, London, Ontario, Canada

    H. Choi, L. Laleye & G. F. Amantea

  2. Faculté des Sciences de L'agriculture et de L'alimentation, Université Laval, G1K 7P4, Québec, Canada

    R. E. Simard

Authors
  1. H. Choi
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  2. L. Laleye
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  3. G. F. Amantea
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  4. R. E. Simard
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Choi, H., Laleye, L., Amantea, G.F. et al. Enzymatic method to determine biomass in skim milk whey permeate medium. Biotechnol Tech 9, 139–144 (1995). https://doi.org/10.1007/BF00224414

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  • DOI: https://doi.org/10.1007/BF00224414

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