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Stabilization of trypsin by modification with comb-shaped copolymers of poly(ethylene glycol) derivative and maleic anhydride

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Biotechnology Techniques

Summary

Trypsin from bovine pancreas was coupled with copolymers of poly(ethylene glycol) derivative and maleic anhydride with the molecular weights of 13 kDa and 100 kDa (activated PM13 and PM100). The modified trypsins were more stable towards autolysis and heat- or urea-treatment than nonmodified trypsin. Stabilization of trypsin caused by the chemical modification with activated PMs is discussed in relation to the protein conformation.

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Authors and Affiliations

  1. Toin Human Science and Technology Center, Department of Materials Science and Technology, Toin University of Yokohama, 1614 Kurogane-cho, Aoba-ku, 225, Yokohama, Japan

    Misao Hiroto, Motoyuki Yamada, Tomoo Ueno, Tohru Yasukohchi, Ayako Matsushima, Yoh Kodera & Yuji Inada

Authors
  1. Misao Hiroto
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  2. Motoyuki Yamada
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  3. Tomoo Ueno
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  4. Tohru Yasukohchi
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  5. Ayako Matsushima
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  6. Yoh Kodera
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  7. Yuji Inada
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Hiroto, M., Yamada, M., Ueno, T. et al. Stabilization of trypsin by modification with comb-shaped copolymers of poly(ethylene glycol) derivative and maleic anhydride. Biotechnol Tech 9, 105–110 (1995). https://doi.org/10.1007/BF00224407

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  • DOI: https://doi.org/10.1007/BF00224407

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