Skip to main content
SpringerLink
Log in

Novel preparation method for surfactant-coated enzymes using W/O emulsion

  • Published:
Biotechnology Techniques

Summary

A novel preparation method for surfactant-coated enzymes has been developed using a W/O emulsion. The enzymatic activity of chymotrypsin in isooctane significantly increased with the coating of surfactants. The surfactant-coated chymotrypsin showed a high enzymatic activity for amidation, although powdered chymotrypsin did not show the activity. Further, the coated enzyme showed a remarkably high storage stability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alexander V. V., Alexander, K. G., Andrey V. L. and Khmelnitsky, Yu. L. Biotechnol. Lett. 1994, 16, 175–178.

    Google Scholar 

  • Dordick, J. S. Enzyme Microb. Technol. 1989, 11, 194–211.

    Google Scholar 

  • Goto, M., Kameyama, H., Goto, M., Miyata, M. and Nakashio, F. J. Chem. Eng. Jpn. 1993, 26, 109–101.

    Google Scholar 

  • Goto, M., Kamiya, N., Miyata, M. and Nakashio, F. Biotechnol. Prog. 1994, 10, 263–268.

    Google Scholar 

  • Goto, M., Goto, M., Kamiya, N., and Nakashio, F. Biotechnol. Bioeng. 1994, 44, in press.

  • Inada, Y., Takahashi, K., Yoshimoto, T., Ajima, A., Matsushita, A., and Saito, Y. Trends Biotechnol. 1986, 4, 190–194.

    Google Scholar 

  • Khmelnitsky, Yu. L., Neverova, I. N., Gedrovich, A. V., Polyakov, V. A., Levashov, A. V. and Martinek, K. Eur. J. biochem. 1992, 210, 751–757.

    Google Scholar 

  • Luisi, P. L., Giomini, M., Pileni, M. P. and Robinson, B. H. Biochem. Biophys. Acta 1988, 947, 209–246.

    Google Scholar 

  • Okahata, Y. and Ijiro, K. J. Chem. Soc., Chem. Commun. 1988, 1392–1394.

  • Tsuzuki, W., Okahata, Y., Katayama, O. and Suzuki, T. J. Chem. Soc., Perkin Trans. 1, 1991, 1245–1247.

  • Zaks, A. and Klibanov, A. M. J. Biol. Chem. 1988, 263, 3194–3201.

    CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Masahiro Goto

    Present address: Department of Chemical Engineering, MIT, 02139, Cambridge, MA, USA

Authors and Affiliations

  1. Department of Chemical Science & Technology, Faculty of Engineering, Kyushu University, Hakozaki, 812, Fukuoka, Japan

    Masahiro Goto, Hiroshi Sumura, Kojiro Abe & Fumiyuki Nakashio

Authors
  1. Masahiro Goto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Sumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kojiro Abe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fumiyuki Nakashio
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goto, M., Sumura, H., Abe, K. et al. Novel preparation method for surfactant-coated enzymes using W/O emulsion. Biotechnol Tech 9, 101–104 (1995). https://doi.org/10.1007/BF00224406

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00224406

Keywords

Search

Navigation