Skip to main content
SpringerLink
Log in

Thermal deactivation affects disruption of Escherichia coli

  • Published:
Biotechnology Techniques

Summary

The effect of thermal deactivation on disruption efficiency and cell-debris size has been investigated for E. coli. Disruption for thermally-deactivated cells was substantially lower than for stationary cells. Cell-debris size was also larger. Thermal deactivation has a significant impact on subsequent downstream operations such as homogenisation and centrifugation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Francis, G.L., Ross, M., Ballard, F.J., Milner, S.J., Senn, C., McNeil, K.A., Wallace, J.C., King, R., Wells, J.R.E. (1992). J. Mol Endocrinol., 8, 213–223.

    Google Scholar 

  • GMAC (1990). Guidelines for Large Scale Work with Genetically Manipulated Organisms., Dept. Adm. Services, Canberra, ACT, Australia.

    Google Scholar 

  • Jin, K., Thomas, O.R.T., Dunnill, P. (1993). Biotech. Bioeng., 43, 455–460.

    Google Scholar 

  • Mackay, B.M., Miles, C.A., Parsons, S.E., Seymour, D.A. (1991). J. Gen. Micro., 137, 2361–2374.

    Google Scholar 

  • Middelberg, A.P.J. 1992, Ph.D. Thesis, University of Adelaide, Australia.

    Google Scholar 

  • Middelberg, A.P.J., O'Neill, B.K., Bogle, I.D.L. (1992a). Trans.I.Chem.E., 70(C), 205–212.

    Google Scholar 

  • Middelberg, A.P.J., O'Neill, B.K., Bogle, I.D.L., Gully, N.J., Rogers, A.H., Thomas, C.J. (1992b). Trans.I.Chem.E., 70(C), 213–218.

    Google Scholar 

  • Mosqueira., F.G., Higgins, J.J., Dunnill, P., Lilly, M.D. (1981). Biotech. Bioeng., 23, 335–343.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Co-operative Research Centre for Tissue Growth and Repair, Department of Chemical Engineering, University of Adelaide, 5005, Adelaide, S.A., Australia

    M. A. Collis, B. K. O'Neill & A. P. J. Middelberg

Authors
  1. M. A. Collis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. K. O'Neill
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. P. J. Middelberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Collis, M.A., O'Neill, B.K. & Middelberg, A.P.J. Thermal deactivation affects disruption of Escherichia coli . Biotechnol Tech 9, 91–94 (1995). https://doi.org/10.1007/BF00224404

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00224404

Keywords

Search

Navigation