Summary
Restriction fragment analysis and heterologous hybridization of chloroplast (cp) DNA was used to develop species-specific markers for P. tabulaeformis, P. yunnanensis and P. massoniana. Fragment patterns created by the BclI and DraI restriction enzymes and hybridization patterns to the psbC and psbD probes were distinctive among the three species. No intraspecific variation was detected with respect to any of the cpDNA markers developed in this study. The cpDNA markers obtained were subsequently used to examine the parentage of P. densata, a putative Tertiary hybrid between P. tabulaeformis and P. yunnanensis. The analysis demonstrated for the first time that P. densata populations accommodate chloroplast genomes of P. tabulaeformis and P. yunnanensis, which strongly supports earlier suggestions of the hybrid origin of this species. It appears that P. densata represents a stabilized natural hybrid that has become adapted to high mountain environments where neither of the parental species can normally grow.
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Alt J, Morris J, Westhoff P, Herrmann RG (1984) Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the “32 kd”-like protein of the photosystem II reaction center in the spinach plastid chromosome. Curr Genet 8:597–606
Anderson E, Stebbins GL (1954) Hybridization as an evolutionary stimulus. Evolution 8:378–388
Barton NH, Hewitt GM (1989) Adaptation, speciation and hybrid zones. Nature 341:497–503
Bohnert HJ, Löffelhardt W (1982) Cyanelle DNA from Cyanaphora paradoxa exists in two forms due to intramolecular recombination. FEBS Lett 150:403–406
Cheng W-C (1930) A study of the Chinese pines. Contrib Biol Lab Sci Soc China Bot Ser 6:5–21 (in Chinese)
Cheng W-C, Fu L-G (1978) Chinese flora. Science Press 7:203–280 (in Chinese)
Cheung WY, Scott NS (1989) A continguous sequence in spinach nuclear DNA is homologous to three separated sequences in chloroplast DNA. Theor Appl Genet 77:625–633
Christensen KI (1987) Taxonomic revision of the Pinus mugo complex and P. × rhaetica (P. mugo × sylvestris) (Pinaceae). Nord J Bot 7:383–408
Critchfield WB (1975) Interspecific hybridization in Pinus: a summary review. In: Proc 14th Meeting Can Tree Impr Assoc. Fredericton, N.B., pp 99–105
Dron M, Rahire M, Rochaix JD (1982) Sequence of the chloroplast DNA region of Chlamydomonas reinhardii containing the gene of the large subunit of ribulose biphosphate carboxylase and parts of its flanking genes. J Mol Biol 162:775–793
Duffield JW (1952) Relationships and species hybridization in the genus Pinus. Z Forstgenet 1:93–97
El-Kassaby YA, Sigurgeirsson A, Szmidt AE (1988) The use of restriction analysis of chloroplast DNA in classifying hybrid spruce seedlots. In: Hällgren J-E (ed) Proc F. Kempe Symposium, Molecular Genetics of Forest Trees, June 14–16, 1988. Umeå, Sweden, pp 33–47
Farjon A (1984) Pines: drawings and descriptions of the genus. E Brill, Leiden
Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Govindaraju DR, Wagner DB, Smith GP, Dancik BP (1988) Chloroplast DNA variation within individual trees of a Pinus banksiana — Pinus contorta sympatric region. Can J For Res 18:1347–1350
Govindaraju DR, Dancik BP, Wagner DB (1989) Novel chloroplast DNA polymorphisms in a sympatric region of two pines. J Evol Biol 2:49–59
Grant V (1981) Plant speciation. New York, Columbia University Press
Guan C-T (1981) Fundamental features of the distribution of Coniferae in Sichuan. Acta Phytotaxon Sin 11:393–407 (in Chinese)
Li B-D, Liu Z-T (1984) The distribution pattern of Pinus yunnanensis. J Yunnan Univ 1:33–46 (in Chinese)
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbour Laboratory, Cold Spring Harbor, N.Y.
Mirov NT (1967) The genus Pinus. The Ronald Press Company, New York
Neale DB, Sederoff RR (1989) Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in loblolly pine. Theor Appl Genet 77:212–216
Neale DB, Wheeler NC, Allard RW (1986) Paternal inheritance of chloroplast DNA in Douglas-fir. Can J For Res 16:1152–1154
Rieseberg LH, Soltis DE, Palmer JD (1988) A molecular reexamination of introgression between Helianthus annuus and H. bolanderi (Compositae). Evolution 42:227–238
Rieseberg LH, Beckstrom-Sternberg S, Doan K (1990) Helianthus annuus ssp. texanus has chloroplast DNA and nuclear ribosomal RNA genes of Helianthus debilis ssp. cucumerifolius. Proc Natl Acad Sci USA 87:593–597
Schaffer HE, Sederoff R (1981) Improved estimation of DNA fragment lengths from agarose gels. Anal Biochem 115:113–122
Sederoff RR, Ronald P, Bedinger P, Rivin C, Walbot V, Bland M, Levings III CS (1986) Maize mitochondrial plasmid S-1 sequences share homology with chloroplast gene psbA. Genetics 113:469–482
Sigurgeirsson A, Szmidt AE, Karpińska B (1990) Alaskan Picea sitchensis populations infiltrated with Picea glauca genes: study using DNA markers. In: Hattemer HH, Fineschi S (eds) Biochemical markers in the population genetics of forest trees. SPB Academic Publ. The Hague, The Netherlands, pp 197–207 (in press)
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Stebbins GL (1959) The role of hybridization in evolution. Proc Am Philos Soc 103:231–251
Stine M, Sears BB, Keathley DE (1989) Inheritance of plastids in interspecific hybrids of blue spruce and white spruce. Theor Appl Genet 78:768–774
Szmidt AE (1990) Phylogenetic and applied studies on chloroplast genome in forest conifers. In: Hattemer HH, Fineschi S (eds) Biochemical markers in the population genetics of forest trees. SPB Academic Publ. The Hague, The Netherlands, pp 185–196 (in press)
Szmidt AE, Lidholm J, Hällgren J-E (1986) DNA extraction and preliminary characterization of chloroplast DNA from Pinus sylvestris and Pinus contorta. In: Lindgren D (ed) Proc F. Kempe Symposium, Provenances and Forest Tree Breeding for High Latitudes, June 10–11, 1986. Umeå, Sweden pp 269–280
Szmidt AE, Aldèn T, Hällgren J-E (1987) Paternal inheritance of chloroplast DNA in Larix. Plant Mol Biol 9:59–64
Szmidt AE, Sigurgeirsson A, Wang X-R, Hällgren J-E, Lindgren D (1988a) Genetic relationships among Pinus species based on chloroplast DNA polymorphism. In: Hällgren J-E (ed) Proc F. Kempe Symposium, Molecular Genetics of Forest Trees, June 14–16, 1988. Umeå, Sweden, pp 33–47
Szmidt AE, El-Kassaby YA, Sigurgeirsson A, Alden T, Lindgren D, Hällgren J-E (1988b) Classifying seedlots of Picea sitchensis and P. glauca in zones of introgression using restriction analysis of chloroplast DNA. Theor Appl Genet 76:841–845
Timmis JN, Scott NS (1983) Sequence homology between spinach nuclear and chloroplast genomes. Nature 305:65–67
Tomioka N, Shinozaki K, Sugiura M (1981) Molecular cloning and characterization of ribosomal RNA genes from a blue-green alga, Anacystis nidulans. Mol Gen Genet 184:359–363
Wagner DB, Furnier GR, Saghai-Maroof MA, Williams SM, Dancik BP, Allard RW (1987) Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. Proc Natl Acad Sci USA 84:2097–2100
Wang X-R, Szmidt AE, Sigurgeirsson A, Karpinska B (1990) A chloroplast DNA story about Asiatic pines. In: Hattemer HH, Fineschi S (eds) Biochemical markers in the population genetics of forest trees. SPB Academic Publ, The Hague, The Netherlands pp 209–216 (in press)
Wang X-R, Szmidt AE, Lewandowski A, Wang Z-R (1990) Evolutionary analysis of Pinus densata Masters, a putative Tertiary hybrid. I. Allozyme variation. Theor Appl Genet 80:635–640
White EE (1990) Chloroplast DNA in Pinus monticola 2. Survey of within-species variability and detection of heteroplasmic individuals. Theor Appl Genet 79:251–255
Wright JW, Gabriel WJ (1958) Species hybridization in the hard pines, series Sylvestres. Silvae Genet 7:109–115
Wu CL (1956) The taxonomic revision and phytogeographical study of Chinese pines. Acta Phytotaxon Sin 5:131–163 (in Chinese)
Zobel B (1951) The natural hybrid between Coulteri and Jeffrey pines. Evolution 5:405–413
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Communicated by P. M. A. Tigerstedt
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Wang, X.R., Szmidt, A.E. Evolutionary analysis of Pinus densata (Masters), a putative Tertiary hybrid.. Theoret. Appl. Genetics 80, 641–647 (1990). https://doi.org/10.1007/BF00224224
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DOI: https://doi.org/10.1007/BF00224224