Summary
Fifty-two introgression lines (BC2F8) from crosses between two Oryza sativa parents and five accessions of O. officinalis were analyzed for the introgression of O. officinalis chromosome segments. DNA from the parents and introgression lines was analyzed with 177 RFLP markers located at approximately 10-cM intervals over the rice chromosomes. Most probe/enzyme combinations detected RFLPs between the parents. Of the 174 informative markers, 28 identified putative O. officinalis introgressed chromosome segments in 1 or more of the introgression lines. Introgressed segments were found on 11 of the 12 rice chromosomes. In most cases of introgression, O. sativa RFLP alleles were replaced by O. officinalis alleles. Introgressed segments were very small in size and similar in plants derived from early and later generations. Some nonconventional recombination mechanism may be involved in the transfer of such small chromosomal segments from O. officinalis chromosomes to those of O. sativa. Some of the introgressed segments show association with genes for brown planthopper (BPH) resistance in some introgressed lines, but not in others. Thus, none of the RFLP markers could be unambiguously associated with BPH resistance.
Similar content being viewed by others
References
Beckman JS, Soller M (1986) Restriction fragment length polymorphism in plant genetic improvement. Oxford Surv Plant Mol Cell Biol 3:197–246.
Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction fragments to high specific activity. Anal Biochem 132:6–13.
Heinrichs EA, Medrano FG, Rapusas HR (1985) Genetic evaluation for insect resistance in rice. International Rice Research Institute, Manila, Philippines.
Ikeda R, Kaneda C (1981) Genetic analysis of resistance to brown planthopper, Nilapravata lugens Stal., in rice. Jpn J Breed 31:279–285.
Ikeda R, Kaneda C (1983) Trisomic analysis of gene Bph1 for resistance to the brown planthopper, Nilapravata lugens Stal., in rice. Jpn J Breed 33:40–44.
Jena KK, Khush GS (1986) Production of monosomic alien addition lines of rice having a single chromosome of O. officinalis. In: Rice Genetics I. International Rice Research Institute, Los Banos, Philippines, pp 199–208.
Jena KK, Khush GS (1989) Monosomic alien addition lines of rice. Production, morphology, cytology and breeding behavior. Genome 32:449–455.
Jena KK, Khush GS (1990) Introgression of genes from Oryza officinalis Wall ex Watt. to cultivated rice, O. sativa L. Theor Appl Genet 80:737–745.
Khush GS (1984) Breeding rice for resistance to insects. Prot Ecol 7:147–165.
Khush GS, Ling KC, Aquino RC, Aguiero VM (1977) Breeding for resistance to grassy stunt in rice. In: Proc 3rd Int.Cong SABRAO. Canberra, Australia. Plant Breed. Papers 1:3–9.
Khush GS, Bacalangco E, Ogawa T (1990) A new gene for resistance to bacterial blight from O. longistaminata. Rice Genet Newsl 7:121–122.
Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337.
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829.
McCouch SR, Khush GS, Tanksley SD (1991) Tagging genes for disease and insect resistance via linkage to RFLP markers. In: Rice Genetics II. International Rice Research Institute, Los Banos, Philippines, pp 443–449.
Neinhuis JT, Helentjaris TM, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27:797–803.
Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction fragment length polymorphism linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet 73:350–356.
Paterson AH, De Verna JW, Lanini B, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete RFLP linkage map. Nature 335:721–726.
Sarfati M, Katan J, Fluhr R, Zamir D (1989) A RFLP marker in tomato linked to the Fusarium oxysporium resistance gene I2. Theor Appl Genet 78:755–759.
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517.
Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato — a re-examination. Theor Appl Genet 75:811–823.
Tanksley SD, Young ND, Paterson A, Bonierbale MW (1989) RFLP mapping in plant breeding: New tools for an old science. Bio/Technology 7:257–264.
Tautz D, Renz M (1983) An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Anal Biochem 132:14–19.
Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:579–585.
Yu ZH, Mackill DJ, Bonman J, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476.
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Jena, K.K., Kochert, G. & Khush, G.S. RFLP analysis of rice (Oryza sativa L.) introgression lines. Theoret. Appl. Genetics 84, 608–616 (1992). https://doi.org/10.1007/BF00224159
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224159