Summary
The progenies of two different rye test-crosses were analyzed for secalin proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using unreduced and reduced aqueous ethanol extracts. Segregation for two high-molecular-weight secalin bands (Glu-R1 or Sec3), one ω-secalin band (Gli-R1 or Sec-1), two 40K γ-secalin bands (Gli-R1 or Sec1) and two ω-type secalin bands (new locus) were studied. One recombinant between ω- and γ-secalins was found in one test-cross. The new locus, designated Gli-R3 or Sec-4, was mapped between Glu-R1 and Gli-R1, more displaced towards Gli-R1. In test-cross 1 recombination between Glu-R1 and Gli-R3 was 33.80±3.22%, and between Gli-R3 and Gli-R1, 12.04±2.21%. In the other test-cross the map distances were relatively similar but smaller, likely due to less recombination within two different species of Secale. Genes coding for 40K γ-secalins at Gli-R1 were likely proximal to the centromere with respect to genes coding for ω-secalins at the same complex locus.
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Benito C, Frade JM, Orellana J, Carrillo JM (1990) Linkage and cytogenetic maps of genes controlling endosperm storage proteins and isozymes in rye (Secale cereale L.). Theor Appl Genet 79:347–352
Bietz JA, Shepherd KW, Wall JS (1975) Cereal single-kernel analysis of glutenin: use in genetics and breeding. Cereal Chem 52:513–532
Carrillo JM, Vázquez JF, Orellana J (1990) Linkage relationships between the loci Sec 1 and Sec 3 in rye (Secale cereale L.). Heredity 64:125–130
Galili G, Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193:293–298
Gupta RB, Shepherd KW (1990 a) Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin. 1. Variation and genetic control of the subunits in hexaploid wheats. Theor Appl Genet 80:65–74
Gupta RB, Shepherd KW (1990 b) Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin. 2. Genetic control of the subunits in species related to wheat. Theor Appl Genet 80:183–187
Jackson EA, Holt LM, Payne PI (1983) Characterisation of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localisation of their controlling genes. Theor Appl Genet 66:29–37
Jackson EA, Holt LM, Payne PI (1985) Glu-B2, a storage protein locus controlling the D group of LMW glutenin subunits in bread wheat (Triticum aestivum). Genet Res 46:11–17
Kasarda DD, Autran J-C, Lew EJ-L, Nimmo CC, Shewry PR (1983) N-terminal amino acid sequences of ω-gliadins and ω-secalins: implications for the evolution of prolamin genes. Biochem Biophys Acta 747:138–150
Laemmli VK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:681–684
Lawrence GJ, Appels R (1986) Mapping the nucleolus organizer region, seed protein loci and isozyme loci in chromosome 1 R in rye. Theor Appl Genet 67:559–568
Lawrence GJ, Shepherd KW (1980) Variation in glutenin protein subunits in wheat. Aust J Biol Sci 33:221–233
Lawrence GJ, Shepherd RW (1981 a) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31
Lawrence GJ, Shepherd KW (1981 b) Inheritance of glutenin protein subunits of wheat. Theor Appl Genet 60:333–337
McIntosh RA (1988) Catalogue of gene symbols for wheat. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Cambridge, pp 1225–1323
Mecham DK, Kasarda DD, Qualset CO (1978) Genetic aspects of wheat gliadin proteins. Biochem Genet 16:831–853
Metakovsky EV, Akhmedov MG, Sozinov AA (1986) Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes. Theor Appl Genet 73:278–285
Orth RA, Bushuk W (1974) VI. Chromosomal location of genes coding for subunits of glutenin of common wheat. Cereal Chem 51:118–126
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Payne PI, Holt LM; Worland AJ, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138
Payne PI, Holt LM, Lister P (1988) Gli-A3 and Gli-B3, two newly designated loci coding for some omega-type gliadins and D subunits of glutenins. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Cambridge, pp 999–1002
Pogna NE, Autran JC, Mellini F, Lafiandra D, Feillet P (1990) Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J Cereal Sci 11:15–34
Shepherd KW (1968) Chromosomal control of endosperm proteins in wheat and rye. In: Finlay KW, Shepherd KW (eds) Proc 3rd Int Wheat Genet Symp. Aust Acad Sci, Canberra, pp 86–96
Shewry PR, Tatham AS (1990) The prolamin storage proteins of cereal seeds: structure and evolution. Biochem J 267:1–12
Shewry PR, Parmar S, Miflin BS (1983) Extraction, separation and polymorphism of the prolamin storage proteins (secalins) of rye. Cereal Chem 60:1–6
Shewry PR, Bradberry D, Franklin J, White RP (1984) The chromosomal locations and linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Theor Appl Genet 69:63–69
Singh NK (1985) The structure and genetic control of endosperm proteins in wheat and rye. Ph.D. thesis, University of Adelaide, Australia
Singh NK, Shepherd KW (1984) Mapping of the genes controlling the high-molecular-weight glutelin subunits of rye on the long arm of chromosome 1R. Genet Res 44:117–123
Sobko TA (1984) Identification of the locus which controls the synthesis of alcohol-soluble endosperm proteins in soft winter wheat. J Agric Sci, Kiev 320:78–80
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Communicated by J.W. Snape
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Carrillo, J.M., Vázquez, J.F. & Orellana, J. Identification and mapping of the Gli-R3 locus on chromosome 1R of rye (Secale cereale L.). Theoret. Appl. Genetics 84, 237–241 (1992). https://doi.org/10.1007/BF00224005
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DOI: https://doi.org/10.1007/BF00224005