Summary
Segments of mitochondrial DNA (mtDNA) carrying the gene for the α-subunit of F1-ATPase (atpA) were detected by Southern hybridization with atpA from pea as probe. In the case of Nicotiana langsdorffii, we identified four fragments that are derived from combinations of two different 5′ and two different 3′ flanking regions of atpA. All four types share the coding region, suggesting that they result from homologous recombination in the coding region of atpA. By contrast, N. glauca generated only one analogous fragment, which indicated the existence of only a single type of atpA in N. glauca. In the case of somatic hybrids obtained by fusion between protoplasts from N. langsdorffii and N. glauca, analysis with EcoRI or HindIII detected three new fragments in addition to the parental fragments. These new fragments can be explained by homologous recombination within the coding region of atpA. Our results show that the coding region of atpA is involved not only in intragenomic homologous recombination but can also be involved in homologous recombination between two parental mitochondrial genomes of somatic hybrids.
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References
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Honda H, Hirai A (1990) A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe. Jpn J Breed 40:339–348
Ichikawa H, Tanno-Suenaga L, Imamura J (1989) Transfer of mitochondria through protoplast fusion. in: Bajaj YPS (ed) Plant protoplasts and genetic engineering. II: Biotechnologyi n agriculture and forestry, vol 9. Springer, Berlin Heidelberg New York, pp 360–375
Issac PG, Brennicke A, Dunbar SM, Leaver CJ (1985) The mitochondrial genome of fertile maize (Zea mays L.) contains two copies of the gene encoding the α-subunit of the F1-ATPase. Curr Genet 10:321–328
Itoh K, Futsuhara Y (1983) Interspecific transfer of only part of the genome by fusion between non-irradiated protoplasts of Nicotiana glauca and X-ray irradiated protoplasts of N. langsdorffii. Jpn J Genet 58:545–553
Kemble RJ (1987) A rapid, single leaf, nucleic acid assay for determining the cytoplasmic organelle complement of rapeseed and related Brassica species. Theor Appl Genet 73: 364–370
Lonsdale DM, Hodge TP, Fauron CM-R (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12:9249–9261
Morikami A, Nakamura K (1987) Structure and expression of pea mitochondrial F1-ATPase α-subunit gene and its pseudogene involved in homologous recombination. J Biochem 101:967–976
Palmer JD, Herbon LA (1987) Unicircular structure of the Brassica hirta mitochondrial genome. Curr Genet 11:565–570
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307:437–440
Quetier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) Molecular form and function of the wheat mitochondrial genome. In: Van Vloten-Doting L, Groot G, Hall T (eds) Molecular form and function of the plant genome. Plenum Press, New York, pp 413–420
Rodermel SR, Bogorad L (1987) Molecular evolution and nucleotide sequences of the maize plastid genes for the α-subunit of CF1 (atpA) and the proteolipid subunit of CF0 (atpH). Genetics 116:127–139
Rothenberg M, Hanson M (1987) Recombination between parental mitochondrial DNA following protoplast fusion can occur in a region which normally does not undergo intragenomic recombination in parental plants. Curr Genet 12:235–240
Schuster W, Brennicke A (1986) Pseudocopies of the ATPase α-subunit gene in Oenothera mitochondria are present on different circular molecules. Mol Gen Genet 204:29–35
Vedel F, Chétrit P, Mathieu C, Pelletier G, Primard C (1986) Several different mitochondrial DNA regions are involved in intergenomic recombination in Brassica napus cybrid plants. Curr Genet 11:17–24
Ward BL, Anderson RS, Bendich AJ (1981) The size of the mitochondrial genome is large and variable in a family of plants (Cucurbitaceae) Cell 25:793–803
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Communicated by I. Potrykus
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Honda, H., Hirai, A. The gene for the α-subunit of ATPase: a site of homologous recombination in plant mitochondrial DNA also functions in somatic hybrid cells. Theoret. Appl. Genetics 84, 33–38 (1992). https://doi.org/10.1007/BF00223978
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DOI: https://doi.org/10.1007/BF00223978