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Similarity of maize and sorghum genomes as revealed by maize RFLP probes

Theoretical and Applied Genetics volume 84, pages 10–16 (1992)Cite this article

Summary

Densely saturated genetic maps of neutral genetic markers are a prerequisite either for plant breeding programs to improve quantitative traits in crops or for evolutionary studies. cDNA and genomic clones from maize were utilized to initiate the construction of a RFLP linkage map in Sorghum bicolor. To this purpose, an F2 population was produced from starting parental lines IS 18729 (USA) and IS 24756 (Nigeria) that were differentiated with regard to many morphological and agronomical traits. A total of 159 maize clones were hybridized to the genomic DNA of the two parents in order to detect polymorphism: 154 probes hybridized to sorghum and 58 out of these were polymorphic. In almost all of the cases hybridization patterns were similar between maize and sorghum. The analysis of the segregation of 35 polymorphic clones in an F2 population of 149 individuals yielded five linkage groups. The three principal ones recall regions of maize chromosomes 1, 3 and 5: in general, colinearity was maintained. A possible inversion, involving a long region of maize chromosome 3, was detected. Simulations were also performed to empirically obtain a value for the lowest number of individuals of the F2 population needed to obtain the same linkage data.

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Affiliations

  1. Department of Genetics and Microbiology, University of Milan, Via Celoria 26, I-20133, Milan, Italy

    G. Binelli, L. Gianfranceschi, M. E. Pè, G. Taramino & E. Ottaviano

  2. International Crop Research Institute for Semi-Arid Tropics, Andra Pradesh, Patancheru, India

    C. Busso & J. Stenhouse

Authors
  1. G. Binelli
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  2. L. Gianfranceschi
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  3. M. E. Pè
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  4. G. Taramino
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  5. C. Busso
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  6. J. Stenhouse
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  7. E. Ottaviano
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Additional information

Prof. E. Ottaviano, to whom this paper is dedicated, suddenly died on June 7th, 1991

Communicated by F. Salamini

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Binelli, G., Gianfranceschi, L., Pè, M.E. et al. Similarity of maize and sorghum genomes as revealed by maize RFLP probes. Theoret. Appl. Genetics 84, 10–16 (1992). https://doi.org/10.1007/BF00223975

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  • DOI: https://doi.org/10.1007/BF00223975

Key words

  • Sorghum
  • Restriction Fragment Length Polymorphism
  • Genetic maps
  • Genomic structure
  • Maize