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An investigation of ageing in human costal cartilage

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Summary

Changes in human costal cartilage with increasing age (2–81 years) have been studied in the optical and electron microscope using routine and histochemical techniques.

Concurrent with increasing age, chondrocytes undergo degeneration which is characterized initially by the accumulation of lipidic material within cells and, subsequently, by the formation of a halo around degenerating chondrocytes. The halo material is composed of electron dense bodies, amorphous material, and collagen fibrils. Both electron dense bodies and the amorphous material are of cellular origin and they have similar histochemical responses.

Using histochemical techniques in the optical and in the electron microscope, it has been shown that chondroitin sulfate decreases with increasing age, while a hyaluronidase resistant material (presumably keratan sulfate) increases, initially in the central zone, and subsequently in the peripheral zones. Hyaluronidase resistant material is minute or absent in the central zone of aged cartilage.

The genesis of collagen fibrils progresses from thin unbanded collagen-like fibrils in the pericellular lacunae of chondrocytes in young specimens to thick fibrils (sometimes in excess of 0.5 μ) with a period of 640 Å in ageing cartilage. Aggregation of collagen fibrils seems to be related at least initially to the preponderance of matrix granules and beaded filaments which have been shown to originate intracellularly in vacuoles formed in degenerating mitochondria. Both of these structures contain glycosaminoglycans and, with increasing age, glycosaminoglycans decrease while collagen fibrils aggregate. In old age, the amorphous material, and possibly the content of disrupting electron dense bodies, seem to give origin to some collagen fibrils. This and other mechanisms of formation of collagen fibrils have been observed and they are discussed.

Calcification of the matrix increases with increasing age and this agrees with previous findings.

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Authors and Affiliations

  1. Istituto di Anatomia Patologica Policlinico Umberto I∘, Viale Regina Elena 324, 00161, Roma, Italia

    L. C. Dearden, E. Bonucci & M. Cuicchio

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  1. L. C. Dearden
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  2. E. Bonucci
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  3. M. Cuicchio
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Supported by grants from the Italian National Research Council. — The authors are indebted to Miss Giuliana Silvestrini and to Mr. Lucio Virgilii for their expert and extensive technical assistance. — To Dr. A. Ascenzi, Director 1° Istituto di Anatomia e Istologia Patologica, and to Dr. C. Cavallero, Director, 2° Istituto di Anatomia e Istologia Patologica, Università di Roma, the senior author would like to express his appreciation for the use of equipment and facilities pursuant to this investigation, while on sabbatical leave from the University of California, Irvine, College of Medicine. — We wish to extend our thanks to the Italian National Research Council for supporting this study.

On sabbatical leave from the University of California, Irvine, College of Medicine.

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Dearden, L.C., Bonucci, E. & Cuicchio, M. An investigation of ageing in human costal cartilage. Cell Tissue Res. 152, 305–337 (1974). https://doi.org/10.1007/BF00223953

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