Summary
The incorporation of 35S-labelled cysteine in the hypothalamic-neurohypophyseal system was studied in normal and adrenalectomized rats and in rats treated with excess hydrocortisone. Labelled cysteine was intraperitoneally administered and grain counts were made of autoradiographs produced from sections of the supraoptic and paraventricular nucleus, median eminence and neurohypophysis of animals killed 45 min., 4 hours and 24 hours after administration of the labelled substance. On the whole, lower incorporation levels of the label were noted in the adrenalectomized rats, compared with the controls. In the rats treated with excess hydrocortisone, the grain counts at 45 min and 4 hours after injection were higher and those at 24 hours were lower than those of the controls.
The findings are discussed, among other things, in terms of rate of uptake vs. time and related to previous reports on the cysteine uptake and neurosecretory activity of the hypothalamic-neurosecretory sytem.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arko, H., Kivalo, E., Rinne, U. K.: Hypothalamo-neurohypophysial neurosecretion after the extirpation of various endocrine glands. Acta endocr. (Kbh.) 42, 293–299 (1963)
Cavallero, C., Dova, E., Rossi, L.: Antidiuretic activity in the neurohypophysis of rats after adrenaleotomy and replacement therapy. J. Endocr. 10, 228–237 (1954)
DeGroot, J.: Neurosecretion in experimental conditions. Anat. Rec. 127, 201–217 (1957)
Eichner, D.: Über den morphologischen Ausdruck funktioneller Beziehungen zwischen Nebennierenrinde und neurosekretorischem Zwischenhirnsystem der Ratte. Z. Zellforsch. 38, 488–508 (1953)
Ficq, A., Flament-Durand, J.: Autoradiography in endocrine research. In: Techniques in endocrine research, p. 73–85, (P. Eckstein and F. Knowles, eds.). London-New York: Academic Press 1963
Flament-Durand, J.: Étude des relations hypothalamo-hypophysaires á l'aide de radioisotopes marqués au Soufre 35. C. R. Acad. Sci. (Paris) 252, 3476–3500 (1961)
Flament-Durand, J.: Contribution á l'étude de la neurosécrétion chez le rat par la méthode autoradiographique. In: Neurosecretion, p. 60–76, (F. Stutinsky, ed.). Berlin-HeidelbergNew York: 1967
Gaunt, R., Lloyd, C. W., Chart, J. J.: The adrenal-neurohypophysial interrelationship. In: The neurohypophysis, p. 233–249, (H. Heller, ed.). London: Butterworths Scientific Publications 1957
Kovacs, K., Bachrach, D.: Hypothalamus and water metabolism. Studies on the antidiuretic substance of the hypothalamus and hypophysis. Acta med. scand. 141, 137–152 (1951)
Rinne, U. K.: Neurosecretory material around the hypophysial portal vessels in the median eminence of the rat. J. endocr. (Kbh) 35, suppl. 57, 1–108 (1960)
Rothballer, A. B.: Changes in the rat neurohypophysis induced by painful stimuli with particular reference to neurosecretory material. Anat. Rec. 115, 21–42 (1953)
Sachs, H.: Vasopressin biosynthesis. II. Incorporation of (35S) cysteine into vasopressin and protein associated with cell fractions. J. Neurochem. 10, 299–306 (1963)
Share, L., Travis, R. H.: Interrelations between the adrenal cortex and the posterior pituitary. Fed. Proc. 20, 1378–1382 (1971)
Sloper, J. C.: Hypothalamo-neurohypophysial neurosecretion. Int. Rev. Cytol. 7, 337–389 (1958)
Sloper, J. C., Arnott, D. J., King, B.C.: Sulphur metabolism in the pituitary and hypothalamus of the rat: a study of radioisotope uptake after the injection of 35S dl-cysteine, methionine, and sodium sulphate. J. Endocr. 20, 9–23 (1960)
Takabatake, Y., Sachs, H.: Vasopressin biosynthesis. III. In vitro studies. Endocrinology 75, 934–942 (1964)
Talanti, S.: The incorporation of 35S-labelled cysteine in the hypothalamic-hypophyseal neurosecretory system of the dehydrated tat. Z. Zellforsch. 115, 110–113 (1971)
Talanti, S.: Effects of thiouracil, excess thyroxine and thyroidectomy on the hypothalamus of the rat with special reference to the hypothalamic-hypophyseal neurosecretory system. Acta Anat. Univ. Helsinkiensis suppl. 1, 1–23 (1972)
Talanti, S., Attila, U.: Incorporation of 35S-labelled cysteine and methionine in the hypothalamic-hypophyseal neurosecretory system of the thyroidectomized rat. Life Sci. 11, 49–54 (1972)
Talanti, S., Pasanen, V.: The incorporation of 35S-labelled cysteine in the hypothalamic-hypophyseal neurosecretory system of rats treated with thiouracil and excess thyroxine. Z. Zellforsch. 88, 220–227 (1968)
Wells, J.: Effect of water deprivation on uptake of dl-cysteine-S35 in the hypothalamo-hypophysial system. Exp. Neurol. 8, 470–481 (1963)
Author information
Authors and Affiliations
Additional information
This study was financially supported by the Sigrid Jusélius Foundation, Helsinki, Finland and the National Research Council for Medical Sciences, Finland.
Rights and permissions
About this article
Cite this article
Talanti, S., Attila, U. Effects of adrenalectomy and excess hydrocortisone on the incorporation of 35S-labelled cysteine in the hypothalamic-neurohypophyseal neurosecretory system of the rat. Cell Tissue Res. 152, 293–297 (1974). https://doi.org/10.1007/BF00223951
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00223951