Abstract
The introgression of rye DNA into the wheat genome was studied using random decamer and specific primers with the polymerase chain reaction (PCR). DNA from paired near-isolines in Chisholm and Arkan backgrounds differing with respect to the presence of a 1 RS.1 BL translocation was amplified with 120 arbitrary sequence primers. Two of the primers (OPR 19 and OPJ07) amplified rye-specific DNA fragments. The OPR19 primer amplified a 1.35-kb fragment that appeared to be specific to the 1 RS.1 BL translocation, based on its presence only in lines carrying the 1 RS. 1 BL translocation. A fragment of the same size was also amplified in 1 RS.1 AL translocation lines. This 1 RS. 1 BL marker locus was designated Ximc 1. The other primer, OPJ07, amplified a 1.2-kb DNA sequence, that was designated Ximc 2, specific to the wheat-rye translocation in various wheat backgrounds. The sequences of the two marker loci were found to be different from each other. The Ximc 1 locus was a low-copy sequence which was also present in Balboa rye genomic DNA. Through the use of specific primers, the presence of the rye-specific marker was confirmed in hexaploid as well as in tetraploid wheat backgrounds. The use of RAPDs for the study of smaller alien introgressions into wheat is discussed.
Similar content being viewed by others
References
Carver BF, Rayburn AL, Hunger RM, Smith EL, Whitmore WE (1993) Registration of lB versus 1RS.1BL near-isoline genetic stocks from two hard red winter wheat populations. Crop Sci 33:1120
Chaparro JX, Werner DJ, O'Malley D, Sederoff RR (1994) Targeted mapping linkage analysis of morphological, isozyme and RAPD markers in peach. Theor Appl Genet 87:805–815
Devos KM, Gale MD (1992) The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84:567–572
Dhaliwal AS, Mares DJ, Marshall DR, Skerritt JH (1988) Protein composition and pentosan content in relation to dough stickiness of 1 B/1 R translocation wheats. Cereal Chem 65:143–149
D'Ovidio R, Tanzarella OA, Porceddu E (1990) Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction. Plant Mol Biol 15:169–171
Friebe B, Hatchett JH, Gill BS, Mukai Y, Sebesta EE (1991) Transfer of Hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations. Theor Appl Genet 83:33–40
Gill BS, Kimber G (1977) Recognition of translocations and alien chromosome transfers in wheat by the Giemsa C-banding technique. Crop Sci 17:264–266
Gonzalez JM, Ferrer E (1993) Random amplified polymorphic DNA analysis in Hordeum species. Genome 36:1029–1031
Haley SD, Miklas PN, Stavely JR, Byrum J, Kelly JD (1993) Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86:505–512
He S, Ohm H, Mackenzie S (1992) Detection of DNA sequence polymorphisms among wheat varieties. Theor Appl Genet 84:573–578
Howes NK, Lukow OM, Dawood MR, Bushuk W (1989) Rapid detection of 1 BL/1 RS chromosome translocation in hexaploid wheats by monoclonal antibodies. J Cereal Sci 10:1–4
Iqbal MJ, Rayburn AL (1994) Stability of RAPD markers for determining cultivar-specific DNA profiles in rye (Secale cereale L). Euphytica 75:215–220
Joshi CP, Nguyen HT (1993) Application of the random amplified polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetraploid wheats. Genome 36:602–609
Kesseli RV, Paran I, Michelmore RW (1994) Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. Genetics 136:1435–1446
King IP, Purdie KA, Rezanoor HN, Koebner RMD, Miller TE, Reader SM, Nicholson P (1993) Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization. Theor Appl Genet 86:895–900
Koebner RMD, Shepherd KW, Appels R (1986) Controlled introgression to wheat of genes from rye chromosome arm 1RS by induction of allosyndesis. 2. Characterisation of recombinants. Theor Appl Genet 73:209–217
Le HT, Armstrong KC, Miki B (1989) Detection of rye DNA in wheat-rye hybrids and a wheat translocation stock using total genomic DNA as a probe. Plant Mol Biol Rep 7:150–158
Lee J-H, Graybosch RA, Lee DJ (1993) Detection of rye chromosome 2R using the polymerase chain reaction and sequence-specific DNA primers. Genome 37:19–22
Lookhart GL, Graybosch R, Peterson J, Lukaszewski A (1991) Identification of wheat lines containing the 1 BL/1 RS translocation by high-performance liquid chromatography. Cereal Chem 68:312–316
Miklas PN, Stavely JR, Kelly JD (1993) Identification and potential use of a molecular marker for rust resistance in common bean. Theor Appl Genet 85:745–749
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993
Penner GA, Chong J, Wight CP, Molnar SJ, Fedak G (1993) Identification of a RAPD marker for the crown rust resistance gene Pc68 in oats. Genome 36:818–820
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissue. In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual A6. Kluwer Academic Publishers, Dordrecht, pp 1–10
Rogowsky PM, Guidet FLY, Langridge P, Shepherd KW, Koebner RMD (1991) Isolation and characterization of wheat-rye recombinants involving chromosome arm 1 DS of wheat. Theor Appl Genet 82:537–544
Rogowsky PM, Sorrels ME, Shepherd KW, Langridge P (1993) Characterization of wheat-rye recombinants with RFLP and PCR probes. Theor Appl Genet 85:1023–1028
Rowland LJ, Levi A (1994) RAPD-based genetic linkage map of blueberry derived from a cross between diploid species (Vaccinium darrowi and V. elliottii). Theor Appl Genet 87:863–868
Torres AM, Weeden NF, Martin A (1993) Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85:937–945
Uphoff H, Wrick G (1992) Random amplified polymorphic DNA (RAPD) markers in sugar beet (Beta vulgaris L.): mapping the genes for nematode resistance and hypocotyl color. Plant Breed 109:168–171
Vierling RA, Nguyen HT (1992) Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. Theor Appl Genet 84:835–838
Weining S, Langridge P (1991) Identification and mapping of polymorphisms in cereals based on polymerase chain reaction. Theor Appl Genet 82:209–216
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Williams JGK, Kubelik AR, Levak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Zeller FJ (1973) l B/1 R wheat-rye substitutions and translocations. Proc 4th Int Wheat Genet Symp, pp 209–221
Zeller FJ, Hsam SLK (dy1983) Broadening the genetic variability of the cultivated wheat by utilizing rye chromatin. Proc 6th Int Wheat Genet Symp, pp 161–173
Author information
Authors and Affiliations
Additional information
Communicated by G. E. Hart
Rights and permissions
About this article
Cite this article
Iqbal, M.J., Rayburn, A.L. Identification of the 1RS rye chromosomal segment in wheat by RAPD analysis. Theoret. Appl. Genetics 91, 1048–1053 (1995). https://doi.org/10.1007/BF00223918
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223918