Skip to main content
Log in

A linkage map with RFLP and isozyme markers for almond

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Inheritance and linkage studies were conducted with seven isozyme genes and 120 RFLPs in the F1 progeny of a cross between almond cultivars ‘Ferragnes’ and ‘Tuono’. RFLPs were detected using 57 genomic and 43 cDNA almond clones. Eight of the cDNA probes corresponded to known genes (extensin, prunin (2), α-tubulin, endopolygalacturonase, oleosin, actin depolymerizing factor and phosphoglyceromutase). Single-copy clones were found more frequently in the cDNA (65%) than in the genomic libraries (26%). Two maps were elaborated, one with the 93 loci heterozygous in ‘Ferragnes’ and another with the 69 loci heterozygous in ‘Tuono’. Thirty-five loci were heterozygous in both parents and were used as bridges between both maps. Most of the segregations (91%) were of the 1∶1 or 1∶1∶1∶1 types, and data were analyzed as if they derived from two backcross populations. Eight linkage groups covering 393 cM in ‘Ferragnes’ and 394 in ‘Tuono’ were found for each map. None of the loci examined in either map was found to be unlinked. Distorted segregation ratios were mainly concentrated in two linkage groups of the ‘Ferragnes’ map.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ahn S, Tanskley SD (1993) Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci 90:7980–7984

    CAS  PubMed  Google Scholar 

  • Arulsekar S, Parfitt DE, Kester DE (1986) Comparison of isozyme variability in peach and almond cultivars. J Hered 77:272–274

    Google Scholar 

  • Arús P, Olarte C, Romero M, Vargas F (1994a) Linkage analysis of ten isozyme genes in F1 segregating almond progenies. J Am Soc Hortic Sci 119:339–344

    Google Scholar 

  • Arús P, Messeguer R, Viruel MA, Tobutt K, Dirlewanger E, Santi F, Quarta R, Ritter E (1994b) The European Prunus mapping project. Progress in the almond linkage map. Euphytica 77:97–100

    Google Scholar 

  • Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898

    Google Scholar 

  • Burr B, Burr RA, Thompson KH, Albertsen MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526

    Google Scholar 

  • Byrne DH (1990) Isozyme varibility in four diploid stone fruits compared with other woody perennial plants. J Hered 81:68–71

    Google Scholar 

  • Cerezo M, Socias i Company R, Arús P (1989) Identification of almond cultivars by pollen isoenzymes. J Am Soc Hortic Sci 114:164–169

    Google Scholar 

  • Chaparro JX, Durham RE, Moore GA, Sherman WB (1987) Use of isozyme techniques to identify peach x ‘Nonpareil’ almond hybrids. HortScience 22:300–302

    Google Scholar 

  • Chaparro JX, Werner DJ, O'Malley D, Sederoff RR (1994) Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach. Theor Appl Genet 87:805–815

    CAS  Google Scholar 

  • Dickson EE, Arumuganathan K, Kresovich S, Doyle JJ (1992) Nuclear DNA content variation within the rosaceae. Am J Bot 79:1081–1086

    Google Scholar 

  • Eldredge L, Ballard R, Baird WV, Abbott A, Morgens P, Callahan A, Scorza R, Monet R (1992) Application of RFLP analysis to genetic linkage mapping in peaches. HortScience 27:160–163

    Google Scholar 

  • Garcia-Mas J, Messeguer R, Arús P, Puigdomènech P (1992) The extensin from Prunus amygdalus. Plant Physiol 100:1603–1604

    Google Scholar 

  • Garcia-Mas J, Messeguer R, Arús P, Puigdomènech P (1995) Molecular characterization of cDNAs corresponding to genes expressed during almond (Prunus amygdalus Batsch) embryo development. Plant Mol Biol 27:205–210

    Google Scholar 

  • Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57

    Google Scholar 

  • Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudotestcross: mapping strategy and RAPD markers. Genetics 137:1121–1137

    CAS  PubMed  Google Scholar 

  • Hauagge R, Rester DE, Asay RA (1987) Isozyme variation among California almond cultivars: I. Inheritance. J Am Soc Hortic Sci 112:687–693

    Google Scholar 

  • Hemmat M, Weeden NF, Manganaris AG, Lawson DM (1994) Molecular marker linkage map for apple. J Hered 85:4–14

    CAS  PubMed  Google Scholar 

  • Jackson JE, Clarke GR (1991) Gene flow in an almond orchard. Theor Appl Genet 82:169–173

    Google Scholar 

  • Kester DE, Asay R (1975) Almonds. In: Janick J, Moore JW (eds) Advances in fruit breeding. Purdue University Press, West Lafayette, La., pp 387–419

    Google Scholar 

  • Lander E, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lee E, Speir J, Gray J, Brady CJ (1990) Homologies to the tomato endopolygalacturonase gen in the peach genome. Plant Cell Environ 13:513–521

    Google Scholar 

  • Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0, 3rd edn. Whitehead Institute Technical Report, Cambridge, Mass.

    Google Scholar 

  • McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829

    CAS  Google Scholar 

  • Messeguer R, Viruel MA, de Vicente MC, Dettori MT, Quarta R (1994) RFLPs. In: Messeguer R (ed). Methods of molecular marker analysis in Prunus. IRTA Technical Report, Barcelona, pp 26–38

    Google Scholar 

  • Reiter RS, Williams JGR, Feldman RA, Rafalski JA, Tingey SV, Scolnik PA (1992) Global and local genome mapping in Arabidopsis thaliana by using recombinant imbred lines and random amplified polymorphic DNAs. Proc Natl Acad Sci USA 89:1477–1481

    Google Scholar 

  • Ritter E, Gebhardt C, Salamini F (1990) Estimation of recombination frequencies and construction of RFLPs map in plants from crosses between heterozygous parents. Genetics 125:645–654

    CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.

    Google Scholar 

  • Sax K (1933) The origin of the Pomoideae. J Am Soc Hortic Sci 30:147–150

    Google Scholar 

  • Spiegel-Roy P (1986) Domestication of fruit trees. In: Barigozzi C (ed) The origin and domestication of cultivated plants. Elsevier, Amsterdam Oxford New York Tokyo, pp 201–212

    Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Stöcker M, Garcia-Mas J, Messeguer R, Arús P, Puigdomènech P (1993) A highly conserved α-tubulin sequence from Prunus amygdalus. Plant Mol Biol 22:913–916

    Google Scholar 

  • Tanksley SD, Miller J, Paterson A, Bernatzky R (1987) Molecular mapping of plant chromosomes. In: Gustafson JP, Apels RA (eds) Chromosome structure in function. Plenum Press, New York, pp 157–173

    Google Scholar 

  • Tanksley SD, Young ND, Patterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7:257–264

    Google Scholar 

  • Torres AM (1990) Isozyme analysis of tree fruits. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Chapman and Hall, London, pp 192–205

    Google Scholar 

  • Vowden C, Ridout M (1994) LINREM. A program for genetic linkage analysis. Technical report. Horticultural Research International, East Mailing, England

    Google Scholar 

  • Watkins R (1976) Cherry, plum, peach, apricot and almond. In: Simmonds NW (ed) Evolution of crop plants. Longman, London New York, pp 242–247

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Viruel, M.A., Messeguer, R., de Vicente, M.C. et al. A linkage map with RFLP and isozyme markers for almond. Theoret. Appl. Genetics 91, 964–971 (1995). https://doi.org/10.1007/BF00223907

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00223907

Key words

Navigation