Abstract
Soybean [Glycine max (L.) Merr.] PI486355 is resistant to all the identified strains of soybean mosaic virus (SMV) and possesses two independently inherited resistance genes. To characterize the two genes, PI486355 was crossed with the susceptible cultivars ‘Lee 68’ and ‘Essex’ and with cultivars ‘Ogden’ and ‘Marshall’, which are resistant to SMV-G1 but systemically necrotic to SMV-G7. The F2 populations and F2∶3 progenies from these crosses were inoculated with SMV-G7 in the greenhouse. The two resistance genes were separated in two F3∶4 lines, ‘LR1’ and ‘LR2’, derived from Essex x PI486355. F1 individuals from the crosses of LR1 and LR2 with Lee 68, Ogden, and ‘York’ were tested with SMV-G7 in the greenhouse; the F2 populations were tested with SMV-G1 and G7. The results revealed that expression of the gene in LR1 is gene-dosage dependent, with the homozygotes conferring resistance but the heterozygotes showing systemic necrosis to SMV-G7. This gene was shown to be an allele of the Rsv1 locus and was designated as Rsv1-s. It is the only allele identified so far at the Rsv1 locus which confers resistance to SMV-G7. Rsv1-s also confers resistance to SMV-G1 through G4, but results in systemic necrosis with SMV-G5 and G6. The gene in LR2 confers resistance to strains SMV-G1 through G7 and exhibits complete dominance. It appears to be epistatic to genes at the Rsv1 locus, inhibiting the expression of the systemic necrosis conditioned by the Rsv1 alleles. SMV-G7 induced a pin-point necrotic reaction on the inoculated primary leaves in LR1 but not in LR2. The unique genetic features of the two resistance genes from PI486355 will facilitate their proper use and identification in breeding and contribute to a better understanding of the interaction of SMV strains with soybean resistance genes.
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References
Bowers GR Jr, Paschal EH II, Bernard RL, Goodman RM (1992) Inheritance of resistance to soybean mosaic virus in ‘Buffalo’ and HLS soybean. Crop Sci 32:67–72
Buss GR, Roane CW, Tolin SA, Chen P (1989a) Inheritance of reaction to soybean mosaic virus in two soybean cultivars. Crop Sci 29:1439–1441
Buss GR, Chen P, Tolin SA, Roane CW (1989b) Breeding soybeans for resistance to soybean mosaic virus. In:Pascale AJ (ed) Proc World Soybean Res Conf, IV. Argentina Soybean Association, Buenos Aries, Argentina, pp 1144–1154
Buzzell RI, Tu JC (1984) Inheritance of soybean resistance to soybean mosaic virus. J Hered 75:82
Buzzell RI, Tu JC (1989) Inheritance of a soybean stem-tip necrosis reaction to soybean mosaic virus. J Hered 80:400–401
Chen P, Buss GR, Tolin SA (1988) Propagation of soybean mosaic virus in soybean callus culture, (abstract) Phytopathology 78:1585
Chen P, Buss GR, Roane CW, Tolin SA (1991) Allelism among genes for resistance to soybean mosaic virus in strain-differential soybean cultivars. Crop Sci 31:305–309
Chen P, Buss GR, Tolin SA (1993) Resistance to soybean mosaic virus conferred by two independent dominant genes in PI486355. J Hered 84:25–28
Chen P, Buss GR, Roane CW, Tolin SA (1994) Inheritance in soybean of resistant and necrotic reactions to soybean mosaic virus strains. Crop Sci 34:414–422
Cho EK, Goodman RM (1979) Strains of soybean mosaic virus: classification based on virulence in resistant soybean cultivars. Phytopathology 69:467–470
Cho EK, Goodman RM (1982) Evaluation of resistance in soybeans to soybean mosaic virus strains. Crop Sci 22:1133–1136
Dunleavy JM (1973) Viral diseases. In:Caldwell BE (ed) Soybeans:improvement, production and uses. American Society of Agronomy, Madison, Wisconsin, pp 505–523
Fraser RSS (1986) Genes for resistance to plant viruses. CRC Crit Rev Plant Sci 3:257–294
Fraser RSS (1990) The genetics of resistance to plant viruses. Annu Rev Phytopathol 28:179–200
Gai J, Hu YZ, Cui ZL, Zhi HJ, Hu WJ, Ren ZJ (1989) An evaluation of resistance of soybean germplasm to strains of soybean mosaic virus. Soybean Sci (China) 8:323–330
Hill JH, Bailey TB, Benner HI, Tachibana H, Durant DP (1987) Soybean mosaic virus: effects of primary disease incidence on yield and quality. Plant Dis 71:237–239
Hunst PL, Tolin SA (1982) Isolation and comparison of two strains of soybean mosaic virus. Phytopathology 72:710–713
Kiihl RAS, Hartwig EE (1979) Inheritance of reaction to soybean mosaic virus in soybeans. Crop Sci 19:372–375
Koshimizu S, Iizuka T (1963) Studies on soybean virus diseases in Japan. Tohoku Nat Agric Exp Stn Bull 27:1–104
Kwon SH, Oh JH (1980) Resistance to a necrotic strain of soybean mosaic virus in soybeans. Crop Sci 20:403–404
Lim SM (1985) Resistance to soybean mosaic virus in soybeans. Phytopathology 75:199–201
Matthews REF (1991) Plant virology. Academic Press, New York
Roane CW, Tolin SA, Buss GR (1983) Inheritance of reaction to two viruses in the soybean cross ‘York’ x ‘Lee 68’. J Hered 74:289–291
Ross JP (1983) Effect of soybean mosaic on component yields from blends of mosaic-resistant and -susceptible soybeans. Crop Sci 3:343–346
Shigemori I (1988) Inheritance of resistance to soybean mosaic virus (SMV) C-strain in soybeans. Jap J Breed 38:346–356
Sinclair JB (ed) (1982) Compendium of soybean diseases. The American Phytopathology Society, St. Paul, Minnesota
Srinivasan I, Tolin SA (1992) Detection of three viruses of clovers by direct tissue immunoblotting. (abstract) Phytopathology 82:721
Takahashi K, Tanaka T, Iida W, Tsuda Y (1980) Studies on virus diseases and causal viruses of soybean in Japan. Bull Tohoku Natl Agric Exp Stn 62:1–130
Tu JC, Buzzell RI (1987) Stem-tip necrosis: a hypersensitive, temperature-dependent, dominant gene reaction of soybean to infection by soybean mosaic virus. Can J Plant Sci 67:661–665
Xu Z, Polston JE, Goodman RM (1986) Identification of soybean mosaic, southern bean mosaic and tobacco ringspot viruses from soybean in the People's Republic of China. Ann Appl Biol 108:51–57
Yan J, Ma Y (1985) Preliminary study on inheritance of resistance to soybean mosaic virus in soybeans. Soybean Sci (China) 4:249–259
Zhang Y, Gai J, Ma Y (1989) Inheritance of resistance to two local soybean mosaic virus strains Sa and Sc in soybeans. Acta Agron Sinica 15:213–220
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Ma, G., Chen, P., Buss, G.R. et al. Genetic characteristics of two genes for resistance to soybean mosaic virus in PI486355 soybean. Theoret. Appl. Genetics 91, 907–914 (1995). https://doi.org/10.1007/BF00223899
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DOI: https://doi.org/10.1007/BF00223899