Abstract
The Owen cytoplasm of male-sterile sugar beet is associated with several alterations of mitochondrial DNA and one additional HindIII site of chloroplast DNA. The region of this HindIII site has been cloned and sequenced. The site maps in a small reading frame (orf32) close to the ycf7 (orf31) gene in the petG-psbE region of chloroplast DNA. Possible functional implications of the results are discussed. The chloroplast RFLP marker described could be useful for studies on chloroplast-mitochondrial interactions, CMS of sugar beet, and the origin of the Owen cytoplasm.
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References
Bonavent JF, Bessone L, Geny A, Bervillé A, Denizot JP, Brian C (1989) A possible origin for the sugar beet cytoplasmic male sterility source Owen. Genome 32:322–327
Boutin V, Pannenbecker G, Ecke W, Schewe G, Saumitou-Laprade P, Jean R, Vernet P, Michaelis G (1987) Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theor Appl Genet 73:625–629
Chen Z, Muthukrishnan S, Liang GH, Schertz KF, Hart GE (1993) A chloroplast DNA deletion located in RNA polymerase gene rpoC2 in CMS lines of sorghum. Mol Gen Genet 236:251–259
Fritzsche K, Metzlaff M, Melzer R, Hagemann R (1987) Comparative restriction endonuclease analysis and molecular cloning of plastid DNAs from wild species and cultivated varieties of the genus Beta (L.) Theor Appl Genet 74:589–594
Göpel G (1970) Plastomabhängige Pollensterilität bei Oenothera. Theor Appl Genet 40:111–116
Haberhausen G, Valentin K, Zetsche K (1992) Organization and sequence of photosynthetic genes from the plastid genome of the holoparasitic flowering plant Cuscuta reflexa. Mol Gen Genet 232:154–161
Haley J, Bogorad L (1990) Alternative promoters are used for genes within maize chloroplast polycistronic transcription units. Plant Cell 2:323–333
Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461–486
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) The complete sequence of the rice (Oriza sativa) chloroplast genome:intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet 217:185–194
Kubo T, Yanai Y, Kinoshita T, Mikami T (1995) The chloroplast trnP-trnW-petG gene cluster in the mitochondrial genomes of Beta vulgaris, B. trigyna and B. webbiana: evolutionary aspects. Curr Genet 27:285–289
Michaelis G, Maggouta F, Mörchen M, Saumitou-Laprade P, Weber B, Weihe A (1995) Molecular studies of CMS in Beta. In: Advances in Plant Breeding Vol. 18-“Genetic Mechanisms for Hybrid Breeding” (U. Kück and G. Wricke eds) Blackwell Wissenschafts-Verlag Berlin, pp 49–58
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1985) Organelle genome diversity in sugar beet with normal and different sources of male-sterile cytoplasms. Theor Appl Genet 71:166–171
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322:572–574
Owen FV (1942) Male sterility in sugar beets produced by complementary effects of cytoplasmic and Mendelian inheritance (abstr) Am J Bot 29:692
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Saumitou-Laprade P, Cuguen J, Vernet P (1994) Cytoplasmic male sterility in plants:molecular evidence and the nucleocytoplasmic conflict. Trends in Evol 9:431–435
Saumitou-Laprade P, Maggouta F, Cuguen J, Wattier R, Van Dijk H, Vernet P, Michaelis G (1993) Cytoplasmic male sterility in Beta vulgaris ssp and the nucleo-cytoplasmic conflict. In: Brennicke A, Kück U (eds) Plant mitochondria. VCH Weinheim, pp 375–383
Saumitou-Laprade P, Pannenbecker G, Boutin-Stadler V, Michaelis G, Vernet P (1991) Plastid DNA diversity in natural populations of Beta maritima showing additional variation in sexual phenotype and mitochondrial DNA. Theor Appl Genet 81:533–536
Senda M, Mikami T, Kinoshita T (1993) The sugar beet mitochondrial gene for the ATPase alpha-subunit: sequence, transcription and rearrangements in cytoplasmic male-sterile plants. Curr Genet 24:164–170
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome:its gene organization and expression. EMBO J 5:2043–2049
Vedel F, Pla M, Vitart V, Gutierres S, Chétrit P, De Paepe R (1994) Molecular basis of nuclear and cytoplasmic male sterility in higher plants. Plant Physiol Biochem 32:601–618
Wakasugi T, Tsudzuki J, Ito S, Shibata M, Sugiura M (1994) A physical map and clone bank of the black pine (Pinus thunbergii) chloroplast genome. Plant Mol Biol Rep 12:227–241
Xue Y, Collin S, Davies DR, Thomas CM (1994) Differential screening of mitochondrial cDNA libraries from male-fertile and cytoplasmic male-sterile sugar-beet reveals genome rearrangements at atp6 and atpA loci. Plant Mol Biol 25:91–103
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Communicated by G. Wenzel
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Ran, Z., Michaelis, G. Mapping of a chloroplast RFLP marker associated with the CMS cytoplasm of sugar beet (Beta vulgaris). Theoret. Appl. Genetics 91, 836–840 (1995). https://doi.org/10.1007/BF00223889
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DOI: https://doi.org/10.1007/BF00223889