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Non-allelic interaction conditioning spikelet sterility in an F2 population of indica/japonica cross in rice

Abstract

Significant segregation of spikelet fertility occurred in an F2 population derived from a spikelet fertility-normal F1 hybrid produced by a cross between ‘Palawan’, a japonica variety, and ‘IR42’, an indica variety. To identify factors controlling the fertility segregation, we used 104 RFLP markers covering all 12 rice chromosomes to investigate the association of spikelet fertility and marker segregation. We found that the segregation of two sets of gene pairs was significantly (P < 0.001) associated with fertility segregation. The first pair of genes was linked to RFLP marker RG778 on chromosome 12 and RFLP markers RG690/RG369 on chromosome 1. A significant reduction in fertility was observed when the plants were homozygote at RG778 with the indica allele as well as homozygote at RG690/RG369 with the japonica allele. The second pair of genes was linked to RG218 on chromosome 12 and RG650 on chromosome 7, respectively. The recombinant homozygote at these two loci showed a significant reduction on spikelet fertility. The non-allelic interaction effect was further modified by a gene linked to RG778, resulting in even lower fertility. The results of this study provides the first evidence of chromosomal localization of sporophytic sterility genes whose interaction can result in a reduction of spikelet fertility in the F2 derived from fertility-normal F1.

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References

  1. Araki H, Ikehashi H, Toya K, Matsumoto S (1990) Development of wide compatibility rice line, ‘Norin PL 9’. JARQ 24:78–81

    Google Scholar 

  2. Causse M, Fulton TM, Cho YG, Ahn SN, Chunwongse J, Wu K, Xiao J, Yu ZH, Ronald PC, Harrington SB, Second GA, McCouch SR, Tanksley SD (1994) Saturated molecular map of the rice genome based on an inter-specific backcross population. Genetics 138:1251–1274

    CAS  PubMed  Google Scholar 

  3. Cowen, NM, Johnson CN, Armstrong K, Miller M, Woosley A, Pescitelli S, Skokut S, Belmar S, Petolino JF (1992) Mapping genes conditioning in vitro androgenesis in maize using RFLP analysis. Theor Appl Genet 84:720–724

    Google Scholar 

  4. Dellaporta SL, Wood J, Hicks JB (1984) Maize DNA miniprep. In: Russell M (ed) Molecular biology of plants. A laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  5. Feinberg AP, Vogelstein B (1984) A technique for radio-labelling DNA restriction fragments to a high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  6. Ikehashi H, Araki H (1986) Genetics of F1 sterility in remote crosses of rice. In: Rice genetics. IRRI, Manila, Phillipines, pp 119–130

    Google Scholar 

  7. Ikehashi H, Araki H (1988) Multiple alleles controlling F1 sterility in remote crosses of rice. Jpn J Breed 38:283–291

    Google Scholar 

  8. Kato S, Kosaka H, Hara S (1928) On the affinity of rice varieties as shown by the fertility of hybrid plants. Bull Sci Fac Agric Kyushu Univ 3:132–147

    Google Scholar 

  9. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  10. Kumar RV, Virmani SS (1992) Wide compatibility in rice (Oryza sativa L.). Euphytica 64:71–80

    Google Scholar 

  11. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 74:543–552

    Google Scholar 

  12. Lin SY, Ikehashi H (1991) A gamete eliminator induced by Est 9 in chromosome 7 of rice (Oryza sativa L.). Jpn J Breed 41:350–351

    Google Scholar 

  13. Lin SY, Ikehashi J, Yanagihara S, Kawashima A (1992) Segregation distortion via male gametes in hybrids between Indica and Japonica or wide-compatibility varieties of rice (Oryza sativa L.). Theor Appl Genet 84:812–818

    Google Scholar 

  14. Morishima H, Sano Y, Oka HI (1992) Evolutionary studies in cultivated rice and its wild relatives. Oxford Surv Evol Biol 8:135–184

    Google Scholar 

  15. Nakagahra M (1972) Genetic mechanism of the distorted segregation of marker genes belonging to the eleventh linkage group in cultivated rice. Jpn J Breed 22:232–238

    Google Scholar 

  16. Oka HI (1957) Genic analysis for the sterility of hybrids between distantly related varieties of cultivated rice. J Genet 55:397–409

    Google Scholar 

  17. Oka HI (1974) Analysis of genes controlling F1 sterility in rice by the use of isogenic lines. Genetics 77:521–534

    Google Scholar 

  18. Oka HI (1978) Phylogenetic differentiation of cultivated rice, XXI. The sprophytic pollen sterility: its genetic basis and intervarietal relationships as shown by F2 sterility. Jpn J Genet 6:397–410

    Google Scholar 

  19. Oka HI (1988) Origin of cultivated rice. Jpn Sci Soc Press, Tokyo

    Google Scholar 

  20. Oka HI, Doida Y (1962) Phylogenetic differentiation of cultivated rice, XX. Analysis of the genetic basis of hybrid breakdown in rice. Jpn J Genet 1:24–35

    Google Scholar 

  21. Sano Y (1990) Genetic analysis of an alien chromosomal segment from Oryza sativa. Jpn J Breed 40[suppl 2]: 306–307

    Google Scholar 

  22. Sano Y (1993) Is an egg-killer present in rice ? Theor Appl Genet 86:1038–1042

    Google Scholar 

  23. SAS Institute (1985) SAS user guide: statistics, basic version, 5th edn. SAS Institute, Cary, N.C.

    Google Scholar 

  24. Shinjyo C (1969) Cytoplasmic-genic male sterility in cultivated rice, Oryza sativa L., II. The inheritance of male sterility. Jpn J Genet 44:149–156

    Google Scholar 

  25. Shinjyo C (1972) Distributions of male sterility inducing cytoplasms and fertility restoring genes in rice, II. Varieties introduced from sixteen countries. Jpn J Breed 22:329–333

    Google Scholar 

  26. Yanagihara S, Kato H, Ikehashi H (1992) A new locus for multiple alleles causing hybrid sterility between an Aus variety and Javanica varieties in rice (Oryza sativa L.). Jpn J Breed 42: 793–801

    Google Scholar 

  27. Zhang G, Lu Y (1993) Genetic studies of the hybrid sterility in cultivated rice (Oryza sativa L.). II. A genic model for F1 pollen sterility. Acta Genet Sinica 20:222–228

    Google Scholar 

  28. Zhang G, Lu Y, Zhang H, Yang J, Lin G (1994) Genetic studies of the hybrid sterility in cultivated rice (Oryza sativa L.). IV. Genotypes for F1 pollen sterility. Acta Genet Sinica 21:34–41

    Google Scholar 

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Communicated by G. Wenzel

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Wu, P., Zhang, G., Huang, N. et al. Non-allelic interaction conditioning spikelet sterility in an F2 population of indica/japonica cross in rice. Theoret. Appl. Genetics 91, 825–829 (1995). https://doi.org/10.1007/BF00223887

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Keywords

  • Oryza sativa L
  • Spikelet fertility
  • Non-allelic interaction
  • Restriction fragment length polymorphism (RFLP)