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Use of Agrobacterium rhizogenes to create transgenic apple trees having an altered organogenic response to hormones

Theoretical and Applied Genetics volume 85, pages 105–109 (1992)Cite this article

Summary

The apple rootstock, M26, was genetically and phenotypically transformed using the Agrobacterium wild-type strain, A4. First, chimeric plants were obtained having transformed roots and normal aerial parts. Transformed plants were then produced through regeneration from transformed roots. Transformation was demonstrated by molecular hybridization and opine analysis. The effects of hormones on organogenesis was altered in transformants: cytokinins were required to form roots, whereas auxin was toxic at the concentration used to induce rooting in the control.

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Affiliations

  1. Faculté des Sciences, Laboratoire de Biologie Végétale, 49045, Angers cedex, France

    Claude Lambert

  2. Laboratoire de Biologie de la Rhizosphère, INRA, 78026, Versailles cedex, France

    David Tepfer

Authors
  1. Claude Lambert
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  2. David Tepfer
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Communicated by I. Potrykus

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Lambert, C., Tepfer, D. Use of Agrobacterium rhizogenes to create transgenic apple trees having an altered organogenic response to hormones. Theoret. Appl. Genetics 85, 105–109 (1992). https://doi.org/10.1007/BF00223851

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  • DOI: https://doi.org/10.1007/BF00223851

Key words

  • Apple
  • Root development
  • Genetic transformation
  • Agrobacterium rhizogenes