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A genetic linkage map of Picea abies Karst., based on RAPD markers, as a tool in population genetics

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Abstract

Norway spruce (Picea abies Karst.) is a most important species among European forest trees for both economical and ecological reasons. However, this species has suffered from a lack of information on the genetic side due to the scarcity of linkage data. In this study we have used a population of 72 megagametophytes from a single tree in a natural Italian stand to produce a genetic linkage map by means of RAPD markers. Ninety-six random decamers used as primers yielded 185 polymorphic loci showing Mendelian inheritance. Analysis of the segregation by multipoint analysis allowed us to define 17 major linkage groups covering a total distance of 3584 cM, with an average spacing between markers of 22 cM. Possible uses of a genetic linkage map with respect to population ecology and genetics are discussed.

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Authors and Affiliations

  1. Department of Genetics and Microbiology, University of Milano, via Celoria 26, 20133, Milano, Italy

    G. Binelli

  2. Institute of Ecology, University of Parma, Viale delle Scienze, 43100, Parma, Italy

    G. Bucci

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  1. G. Binelli
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  2. G. Bucci
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Communicated by P. M. A. Tigerstedt

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Binelli, G., Bucci, G. A genetic linkage map of Picea abies Karst., based on RAPD markers, as a tool in population genetics. Theoret. Appl. Genetics 88, 283–288 (1994). https://doi.org/10.1007/BF00223633

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  • DOI: https://doi.org/10.1007/BF00223633

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