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Increase in hepatic microsomal lipid peroxidation mediated by α-adrenergic system under cold stress and noradrenaline treatment

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Abstract

Lipid peroxidation measured both by the formation of malondialdehyde and by oxygen uptake in presence of NADPH, Fe2− and ADP in hepatic microsomes increased on cold exposure and decreased on heat exposure of rats. Malondialdehyde content of isolated microsomes also showed similar changes. Treatment of animals with noradrenaline or a a-adrenergic agonist, phenylephrine, increased lipid peroxidation which was prevented by simultaneous treatment with cycloheximide, a protein synthesis inhibitor. Cold- and noradrenaline-induced increases were not found in animals pretreated with a-adrenergic receptor antagonist, phenoxybenzamine, but not with propranolol, a β-adrenergic blocking agent. The concentration of the microsomal cytochromes P-450 and b 5 remained unaffected under these conditions but the activity of NADPH-cytochrome c reductase showed parallel changes. These observations suggest a role for lipid peroxidation in cellular thermogenesis in liver tissue.

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References

  1. Hochachka PW: Regulation of heat production at the cellular level. Fed Proc (USA) 33: 2162–2169, 1974

    Google Scholar 

  2. Smith RE, Howirtz BA: Brown fat and thermogenesis. Physiol Rev 49: 330–425, 1969

    Google Scholar 

  3. Chaffee RRJ, Roberts JC: Temperature acclimation in birds and animals. Ann Rev Physiol 33: 155–202, 1971

    Google Scholar 

  4. Jansky L: Non-shivering thermogenesis and its thermoregulatory significance. Biol Rev 48: 85–132, 1973

    Google Scholar 

  5. Himms-Hagen J: Cellular Thermogenesis. Ann Rev Physiol 38: 315–351, 1976

    Google Scholar 

  6. Morrison GR, Brook FE, Sobral DT, Shank RE: Coldacclimatization and intermediary metabolism of carbohydrates. Arch Biochem Biophys 114: 494–501, 1966

    Google Scholar 

  7. Himms-Hagen J: Sympathetic regulation of metabolism. Pharmacol Rev 19: 367–461, 1967

    Google Scholar 

  8. Zeisberger E, Bruck K: Cental effects of noradrenaline on the control of body temperature in the guinea-pig. Pflugers Arch 322: 152–166, 1971

    Google Scholar 

  9. Bartunkova R, Jansky L, Mejsnar J: Nonshivering thermogenesis and cold adaptation. In: ‘Nonshivering Thermogenesis’ Publ. Academia, Prague, Czechoslovakia, pp 39–56, 1971

    Google Scholar 

  10. Bruck K, Baum E, Schwennicke HP: The significance of central adrenergic a-receptive structures in the control of thermogenesis and in cold adaptation. Israel J Med Sci 12: 1103–1106, 1976

    Google Scholar 

  11. Zeisberger E, Bruck K: Alteration of shivering threshold in cold- and warm-adapted guinea pigs following intrapthalmic injection of noradrenaline and of an adrenergic alphareceptor blocking agent. Pflugers Arch 362: 113–119, 1976

    Google Scholar 

  12. Zeisberger E, Bruck K: Cold-adaptive modifications in man induced by repeated short-term cold exposures and during a 10-day and night cold-exposure. Pflugers Arch 363: 125–133, 1976

    Google Scholar 

  13. Sitaramam V, Meera Rau, Ramasarma T: Influence of some drugs on body temperature and induction of hepatic tryptophan pyrrolase in animals exposed to cold. Indian J Expt Biol 15: 98–104, 1977

    Google Scholar 

  14. Dhawan BN, Dua PR: Evidence for the preesence of aadrenoreceptor in the central thermoregulatory mechanism of rabbits. Brit J Pharmacol 43: 497–503, 1971

    Google Scholar 

  15. Lomax P, Foster RS, Kirkpatrick WE: Cholinergic and adrenergic interactions in the thermoregulatory centers of the rat. Brain Res 14: 431–438, 1969

    Google Scholar 

  16. Aithal HN, Ramasarma T: Changes in the liver mitochondrial oxidation of succinate during cold exposure. Biochem J 123: 677–682, 1971

    Google Scholar 

  17. Swaroop A, Ramasarma T: Changes in mitochondrial oxidative metabolism under conditions of heat stress. Indian J Biochem Biophys 19: 382–387, 1982

    Google Scholar 

  18. Puranam RS, Gupta SD, Shivaswamy V, Ramasarma T, Ramakrishna Kurup CK: Regulation of electron transport in hepatic mitochondria on acclimation of animals to heat stress. Indian J Biochem Biophys 24: 314–320, 1987

    Google Scholar 

  19. Lindberg O: ed. Brown Adipose Tissue, Elsevier, New York, 1970

    Google Scholar 

  20. Cannon B, Nedergaard J: The biochemistry of an inefficient tissue. Essays Biochem 20: 110–163, 1987

    Google Scholar 

  21. Ricquier D, Gerrias C, Kader JC, Hemon P: Partial purification of guanosine-5-diphosphate -agarose affinity chromatography of the 32000 molecular wt. polypeptide from mitochondria of brown adipose tissue. FEBS Lett 101: 3538, 1979

    Google Scholar 

  22. Lin C, Klingerberg M: Characteristics of the isolated purine nucleotide binding protein from brown fat mitochondria. Biochemistry 21: 2950–2956, 1982

    Google Scholar 

  23. Cannon B, Hedin A, Nedergaard J: Exclusive occurrence of thermogenin antigen in brown adipose tissue. FEBS Lett 150: 129–132, 1982

    Google Scholar 

  24. Mory G, Bouillard F, Combes-George M, Ricquier D: Noradrenaline controls the concentration of uncoupling protein in brown adipose tissue. FEBS Lett 166: 393–396, 1984

    Google Scholar 

  25. Ramasarma T, Seshadri Sekhar B, Ramakrishna Kurup CK: Cellular Thermogenesis. In: T. Ozawa, S. Papa (eds.), Bioenergetics: Structure and Function of Energy Transducing Systems. Japan Sci Soc Press, Tokyo/Springer-Verlag, Berlin, pp 225–233, 1987

    Google Scholar 

  26. Omura T, Sato R: The carbon monoxide-binding pigment of liver microsomes. Il Solubilization, purification and proporties. J Biol Chem 239: 2379–2385, 1964

    Google Scholar 

  27. Omura T, Sato R, Cooper DY, Rosenthal O, Estabrook RW: Function of cytochrome P-450 of microsomes. Fed Proc (USA) 24: 1181–1189, 1965

    Google Scholar 

  28. Ruckpaul K, Rein H: In: ‘Cytochrome P-450’ Akademic-Verlag, Berlin, 1984

    Google Scholar 

  29. Hochstein P, Ernster L: ADP-activated lipid peroxidation coupled to the TPNH oxidase system of microsomes. Biochem Biophys Res Commun 12: 388–394, 1963

    Google Scholar 

  30. Gornall AG, Bardawill CJ, David MM: Determination of serum proteins by means of biuret reaction. J Biol Chem 177: 751–766, 1949

    Google Scholar 

  31. Ramasarma T, Muakkassa-Kelly S, Hochstein P: Inhibition of microsomal lipid peroxidation by cytosolic protein in presence of ADP and high concentration of Fe2+. Biochim Biophys Acta 796: 243–250, 1984

    Google Scholar 

  32. Buege JA, Aust SD: Estimation of malondialdehyde. Methods Enzymol 52: 302–30, 1978

    Google Scholar 

  33. Estabrook RW, Werringloer J: Estimation of cytochrome P-450. Methods Enzymol 52: 212–220, 1978

    Google Scholar 

  34. Schmelck PH, Hanoune J: The hepatic adrenergic receptors. Mol Cell Biochem 33: 35–48, 1980

    Google Scholar 

  35. Ramasarma T, Shivaswamy V, Meera Rau: Adenosine and chlorpromazine induced hypothermia. In: B. Cox et al. (ed.), ‘Thermoregulatory Mechanisms and their Therapeutic Implications. Publ S Krager AG, pp 229–232, 1980

  36. Reinhart PH, Taylor WM, Bygrave FL: The role of calcium ions in the mechanism of action of a-adrenergic agonists in rat liver. Biochem J 223: 1–13, 1984

    Google Scholar 

  37. Kappus H: Lipid peroxidation: Mechanisms, Analysis, Enzymology and Biological Relevance. In: H. Seis (ed.), ‘Oxidation Stress’. Acad Press, London, pp 273–310, 1985

    Google Scholar 

  38. Gibson DD, Hornbrook KR, McCay PB: Glutathionedependent inhibition of lipid peroxidation by a soluble heat-labile factor in animal tissues. Biochem Biophys Acta 620: 572–582, 1980

    Google Scholar 

  39. Haenen GRMM, Bast A: Protection against lipid peroxidation by a microsomal glutathione-dependent labile factor. FEBS Lett 159: 24–28, 1983

    Google Scholar 

  40. Reddy CC, Scholz RW, Thomas CE, Massaro EJ: Vitamin E dependent reduced glutathione inhibition of rat liver microsomal lipid peroxidation. Life Sci 31: 571–576, 1982.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Indian Institute of Science, 560 012, Bangalore, India

    B. Seshadri Sekhar, C. K. Ramakrishna Kurup & T. Ramasarma

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  1. B. Seshadri Sekhar
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  2. C. K. Ramakrishna Kurup
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  3. T. Ramasarma
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Seshadri Sekhar, B., Ramakrishna Kurup, C.K. & Ramasarma, T. Increase in hepatic microsomal lipid peroxidation mediated by α-adrenergic system under cold stress and noradrenaline treatment. Mol Cell Biochem 94, 61–70 (1990). https://doi.org/10.1007/BF00223563

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  • DOI: https://doi.org/10.1007/BF00223563

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