Abstract
Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex with oligonucleotides containing a TAATGARAT element.
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Abbreviations
- AcNPV:
-
Autographica californica nuclear polyhedrosis virus
- HSV:
-
Herpes Simplex Virus
- IE:
-
Immediate Early
- moi:
-
multiplicity of infection
- Sf9:
-
Spodoptera frugiperda cells
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Capone, J.P., Werstuck, G. Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector. Mol Cell Biochem 94, 45–52 (1990). https://doi.org/10.1007/BF00223561
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DOI: https://doi.org/10.1007/BF00223561