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Expression of the C3-C4 intermediate character in somatic hybrids between Brassica napus and the C3-C4 species Moricandia arvensis

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Abstract

The wild crucifer Moricandia arvensis is a potential source of alien genes for the genetic improvement of related Brassica crops. In particular M. arvensis has a C3-C4 intermediate photosynthetic mechanism which results in enhanced recapture of photorespired CO2 and may increase plant water-use efficiency. In order to transfer this trait into Brassica napus, somatic hybridisations were made between leaf mesophyll protoplasts from cultured M. arvensis shoot tips and hypocotyl protoplasts from three Brassica napus cultivars, ‘Ariana’, ‘Cobra’ and ‘Westar’. A total of 23 plants were recovered from fusion experiments and established in the greenhouse. A wide range of chromosome numbers were observed among the regenerated plants, including some apparent mixoploids. Thirteen of the regenerated plants were identified as nuclear hybrids between B. napus and M. arvensis on the basis of isozyme analysis. The phenotypes of these hybrids were typically rather B. napus-like, but much variability was observed, including variation in flower colour, leaf shape and colour, leaf waxiness, fertility and plant vigour. CO2 compensation point measurements on the regenerated plants demonstrated that 3 of the hybrids express the M. arvensis C3-C4 intermediate character at the physiological level. Semi-thin sections through leaf tissues of these 3 plants revealed the presence of a Kranz-like leaf anatomy characteristic of M. arvensis but not found in B. napus. This is the first report of the expression of this potentially important agronomic trait, transferred from Moricandia, in M. arvensis x B. napus hybrids.

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Author notes

  1. T. Murata

    Present address: Faculty of Agriculture, Kyushu Tokai University, Kawayo, Choyo, Aso, 869-14, Kumamoto, Japan

Authors and Affiliations

  1. Brassica and Oilseeds Research Department, John Innes Centre, Norwich Research Park, NR4 7UJ, Norwich, UK

    C. M. O'Neill, T. Murata, C. L. Morgan & R. J. Mathias

Authors
  1. C. M. O'Neill
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  2. T. Murata
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  3. C. L. Morgan
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  4. R. J. Mathias
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Communicated by K. Glimelius

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O'Neill, C.M., Murata, T., Morgan, C.L. et al. Expression of the C3-C4 intermediate character in somatic hybrids between Brassica napus and the C3-C4 species Moricandia arvensis . Theoret. Appl. Genetics 93, 1234–1241 (1996). https://doi.org/10.1007/BF00223455

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  • DOI: https://doi.org/10.1007/BF00223455

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