Abstract
We report here the RFLP mapping of quantitative triat loci (QTLs) that affect some important agronomic traits in cultivated rice. An anther culturederived doubled haploid (DH) population was established from a cross between an indica and a japonica rice variety. On the basis of this population a molecular linkage map comprising 137 markers was constructed that covered the rice genome at intervals of 14.8cM on average. Interval mapping of the linkage map was used to locate QTLs for such important agronomic traits as heading date, plant height, number of spikelets per panicle, number of grains Per panicle, 1000-grain weight and percentage of seed set. Evidence of genotype-byenvironment interaction was found by comparing QTL maps of the same population grown in three diverse environments. A total of 22 QTLs for six agronomic traits were detected that were significant in at least one environment, but only 7 were significant in all three environments, 7 were significant in two environments and 8 could only be detected in a single environment. However, QTL-by-environment interaction was traitdependent. QTLs for spikelets and grains per panicle were common across environments, while traits like heading date and plant height were more sensitive to environment.
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References
Ahn SN, Bollich CN, McClung AM, Tanksley SD (1993) RFLP analysis of genomic regions associated with cooked-kernel elongation in rice. Theor Appl Genet 87:27–32
Backes G, Graner A, Foroughi-Wehr B, Fischbeck G, Wenzel G, Johoor A (1995) Localization of quantitative trait loci (QTL) for agronomic important chracters by the use of a RFLP map in barley (Hordeum vulgare L.). Theor Appl Genet 90:294–302
Barua UM, Chalmers KJ, Thomas WTB, Hackett CA, Lea V, Jack P, Forster BP, Waugh R, Powell W (1993) Genome 36:1080–1087
Burr D, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Causse M, Fulton T, Cho Y, Ahn S, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald P, Harrington S, Second G, McCouch S, Tanksley S (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251–1274
Freyer R, Douches DS (1994) Development of a model for markerassisted selection of specific gravity in diploid potato across environments. Crop Sci 34:1361–1368
Heun M (1992) Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map. Genome 35:1019–1025
Kinoshita T, Takashashi M (1991) The one hundredth report of genetical studies on rice plant-linkage studies and future prospects. J Fac Agric Hokkaido Univ 65:1–61
Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio B, Shomura A, Shimizu T, Lin S-Y, Inoue T, Fukuda A, Shimano T, Kuboki Y, Toyama T, Miyamoto Y, Kirihara T, Hayasaka K, Miyao A, Monna L, Zhong HS, Tamura Y, Wang Z-X, Momma T, Umehara Y, Yano M, Sasaki T, Minobe Y (1994) A 300-kilobase interval genetic map of rice including 883 expressed sequences. 8:365–372
Lander ES, Botstein D (1989) Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–19
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg L (1987) MAPMAKER: an interacive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lefebvre V, Palloix A, Caranta C, Pochard E (1995) Construction of intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies. Genome 38:112–121
Li ZK, Pinson SRM, Stensel JW, Park WD (1995a) Identification of quantitative trait loci (QTLs) for heading date and plant height in cultivated rice (Oryza sativa L.). Theor Appl Genet 91:374–381
Li ZK, Pinson SRM, Marchetti MA, Stensel JS, Park WD (1995b) Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani). Theor Appl Genet 91:382–388
Lincoln SE, Daly MJ, Lander ES (1993a) Constructing genetic linkage maps with MAPMAKER/EXP version 3.0: a tutorial and reference manual. 3rd edn. Whitehead Institute for Biometrical Research Cambridge, Mass.
Lincoln SE, Daly MJ, Lander ES (1993b) Mapping genes controlling quantitative traits using MAPMAKER/QTL version 1.1: a tutorial and reference manual, 2nd edn. Whitehead Institute for Biometrical Research, Cambridge, Mass.
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Mohan M, Nair S, Bentur JS, Rao UP, Bennett J (1994) RFLP and RAPD mapping of the rice Gm2 gene that confers to biotype 1 of gall midget (Orseolia oryzae). Theor Appl Genet 87:782–788
Paterson AH, Lander ES, Had JD, Paterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander EC, Tanksley SD (1991) Mendelian factors underlying quantitiative traits in tomato: comparison across species, generations and environments. Genetics 127:181–197
Stuber CW (1992) Biochemical and molecular markers in plant breeding. Plant Breed Rev 9:37–61
Stuber CW, Lincoln SE, Wolff DW, Helentjaris T, Lander ES (1992) Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics 132:823–839
Toroser D, Thormann CE, Osborn TC, Mithen R (1995) RFLP mapping of quantitative trait loci controlling seed aliphaticglucosinolate content in oilseed rape (Brassica napus L.) Theor Appl Genet 91:802–808
Uzunova M, Ecke W, Weissleder K, Röbbelen G (1995) Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90:194–204
Xiao J, Li J, Yuan L, Tanksley SD (1994) Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers. Genetics 140:745–754
Xu YB, Shen ZT, Chen Y, Zhu LH (1995) Distorted segregations of RFLP markers and their distribution on chromosomes in an indica/japanica F2 population of rice (Oryza sativa L.). Acta Bot Sinica 37:91–96
Wang G, Mackill DJ, Bonman JM, McCouch SR, Champoux MC et al. (1994) RFPL mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136:1421–1434
Young ND, Tanksley SD (1989) Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet 77:95–101
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Communicated by G. Wenzel
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Lu, C., Shen, L., Tan, Z. et al. Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population. Theoret. Appl. Genetics 93, 1211–1217 (1996). https://doi.org/10.1007/BF00223452
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DOI: https://doi.org/10.1007/BF00223452