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Genetic analysis of organogenesis in the cotyledons of zygotic embryos of sunflower (Helianthus annuus L.)

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Abstract

Crosses were made between five cytoplasmic male-sterile and five restorer sunflower inbred lines. Twenty-five F1 hybrids and their parents were studied for their organogenesis ability in a randomized block design with four replications. Each replication per genotype consisted of ten petri dishes with four expiants. Regeneration medium consisted of full MS medium modified by the addition of hormones and solidified with 6 g/l agar. Statistical analysis showed that both general and specific combining abilities were significant for all of the organogenesis parameters studied, and both showed several significant positive or negative values. General combining ability values were usually higher than those of specific combining ability, indicating the importance of additive genetic control for organogenesis parameters in sunflower. Narrow-sense heritability for the number of explants producing shoots and roots was 65.8%, which suggests that organogenesis of currently inferior inbred lines in sunflower should be improved in a crossing program.

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References

  • Bullock WP, Baenziger PS (1982) Anther culture of wheat (Triticum aestivum L.) F1S and their reciprocal crosses. Theor Appl Genet 62:155–159

    Google Scholar 

  • Ceriani MF, Hopp HE, Hahne G, Escandon AS (1992) Cotyledons: an explant for routine regeneration of sunflower plants. Plant Cell Physiol 33:157–164

    Google Scholar 

  • Chraibi B, Latche A, Roustan JP, Fallot J (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus L.by the ethylene inhibitors, silver and cobalt. Plant Cell Rep 10:204–207

    Google Scholar 

  • Chraibi MB, Castelle JC, Latché A, Roustan JP, Fallot J (1992) A genotype-independant system of regeneration from cotyledons of sunflower (Helianthus annuus L.). The role of ethylene. Plant Sci 86:215–221

    Google Scholar 

  • Espinasse A, Lay C, Volin J (1989) Effects of growth regulator concentrations and explant size on shoot organogenesis from callus derived from zygotic embryos of sunflower (Helianthus annuus L.). Plant Cell Tissue Org Cult 17:171–181

    Google Scholar 

  • Finer JJ (1987) Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose containing medium. Plant Cell Rep 6:372–374

    Google Scholar 

  • Freyssinet M, Freyssinet G (1988) Fertile plant regeneration from sunflower (Helianthus annuus L.) immature embryos. Plant Sci, 56:177–181

    Google Scholar 

  • Gallais A (1990) Théorie de la sélection en amélioration des plantes. Masson, Paris, France

    Google Scholar 

  • Garretsen F, Keuls M (1978) A general method for the analysis of genetic variation in North Carolina II (NCII) designs. Part II: procedures and general formulas for the fixed model. Euphytica 27:49–68

    Google Scholar 

  • Ghaemi M, Sarrafi A (1993) Analysis of anther culture to measure genetic variability for embryogenesis in tetraploid wheat (Triticum turgidum var.). J Genet Breed 47:295–298

    Google Scholar 

  • Greco B, Tanzarella OA, Carrozzo G, Blanco A (1984) Callus induction and shoot regeneration in sunflower (Helianthus annuus L.). Plant Sci 36:73–77

    Google Scholar 

  • Hernandez-Fernandez MM, Christie BR (1989) Inheritance of somatic embryogenesis in alfalfa (Medicago savita L.). Genome 32:318–321

    Google Scholar 

  • Kempthorne O (1957) An introduction to genetic statistics. Wiley and Sons, New York, USA

    Google Scholar 

  • Keyes GJ, Collins GB, Taylor NL (1980) Genetic variation in tissue culture of red clover. Theor Appl Genet 58:265–271

    Google Scholar 

  • Lazar MD, Baenziger PS, Schaeffer GW (1984) Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther cultures. Theor Appl Genet 68:131–134

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioessay with tobacco tissue culture. Physiol Plant 15:473–479

    Google Scholar 

  • Ou G, Wang WC, Nguyen HT (1989) Inheritance of somatic embryogenesis and organ regeneration from immature embryo cultures of winter wheat. Theor Appl Genet 78:137–142

    Google Scholar 

  • Paterson KE, Everett NP (1985) Regeneration of Helianthus annuus inbred plants from callus. Plant Sci 42:125–132

    Google Scholar 

  • Pélissier B, Bouchefra O, Pepin R, Freyssinet G (1990) Prodcuction of isolated somatic embryos from sunflower thin cell layers. Plant Cell Rep 9:47–50

    Google Scholar 

  • Power CJ (1987) Organogenesis from Helianthus annuus inbreds and hybrids from the cotyledons of zygotic embryos. Am J Bot 74:497–503

    Google Scholar 

  • Prado E, Bervillé A (1990) Induction of somatic embryo development by liquid culture in sunflower (Helianthus annuus L.). Plant Sci 67:73–82

    Google Scholar 

  • Quimio CA, Zapata FJ (1990) Diallel analysis of callus induction and green-plant regeneration in rice anther culture. Crop Sci 30:188–192

    Google Scholar 

  • Raquin C (1982) Genetic control of embryo production and embryo quality in anther culture of Petunia. Theor Appl Genet 63:151–154

    Google Scholar 

  • Wilcox McCann A, Cooley G, Van Dresser J (1988) A system for routine planted regeneration of sunflower (Helianthus annuus L.) from immature embryo-derived callus. Plant Cell Tissue Org Cult 14:103–110

    Google Scholar 

  • Witrzens B, Scowcroft WR, Downes RW, Larkin PJ (1988) Tissue culture and plant regeneration from sunflower (Helianthus annuus) and interspecific hybrids (H. tuberosus x H. annuus). Plant Cell Tissue Org Cult 13:61–76

    Google Scholar 

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Communicated by G. Wenzel

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Sarrafi, A., Roustan, J.P., Fallot, J. et al. Genetic analysis of organogenesis in the cotyledons of zygotic embryos of sunflower (Helianthus annuus L.). Theoret. Appl. Genetics 92, 225–229 (1996). https://doi.org/10.1007/BF00223379

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  • DOI: https://doi.org/10.1007/BF00223379

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