Skip to main content
SpringerLink
Log in

Effect of a single dose of inducers and inhibitors on the rate of synthesis of cytochromes and reductases in liver organelles

  • General Articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Measurement of the effect of drugs on the in vivo rates of synthesis of rabbit liver organelle bound proteins were measured following individual treatments with the inducers phenobarbital, 3-methylcholanthrene and PCB (a mixture of polychlorinated biphenyls) and the inhibitors, cycloheximide, aflatoxin 13, chloramphenicol and actinomycin D. Following their isolation from a homogenate containing the combined livers of 14C-leucine injected experimental animals and 3H-leucine injected control animals, purified fractions of the following proteins were prepared: microsomal cytochrome b5, cytochrome P-450, NADH-cytochrome b5 reductase, NADPH-cytochrome P-450 reductase and proteolipids, outer mitochrondrial membrane cytochrome b5, NADH-cytochrome b5 reductase and proteolipids, inner mitochrondrial membrane cytochrome c, NADH dehydrogenase and proteolipids, intermitochrondrial membrane cytochrome b5 and circulating serum albumin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kuntzman, R. 1969. Ann. Rev. Pharmacol. 9, 21–27.

    Google Scholar 

  2. Kuriama, T., Omura, T., Siekevitz, P. and Palade, G. E. 1969. J. Biol. Chem. 244, 2017–2026.

    Google Scholar 

  3. Arias, I. M., Doyle, D. and Schimke, R. T. 1969. J. Biol. Chem. 244, 3303–3315.

    Google Scholar 

  4. Dehlinger, P. J. and Schimke, R. T. 1972. J. Biol. Chem. 247, 1257–1264.

    Google Scholar 

  5. Raw, I. 1978. Fed. Proc., 37, 1544.

    Google Scholar 

  6. Schenkman, J. B. and Centi, D. L. 1978. Methods in Enzymology (Colowick S. P. and Kaplan N. O.), ed. 52, 83–89, Academic Press, N.Y.

  7. Fukushima, K. and Sato, R. 1973. Biochem. J. (Tokyo), 74,161–173.

    Google Scholar 

  8. Raw, I. 1978. Biochem. Biophys. Res. Commun. 81, 1294–1297.

    Google Scholar 

  9. Ozols, J. 1974. Biochemistry 13, 426–433.

    Google Scholar 

  10. Hara, T. and Minakami, S. 1970. J. Biochem. (Tokyo) 67,741–743.

    Google Scholar 

  11. Fujita, T., Showman, D. W. & Mannering, G. J. 1973. J. Biol. Chem. 248, 2192–2201.

    Google Scholar 

  12. Imai, Y., and Sato, R. 1974. Biochem. Biophys. Res. Commun. 60, 8–14.

    Google Scholar 

  13. Yasukochi, Y. and Masters, B.S.S. 1976. J. Biol. Chem. 254,5337–5343.

    Google Scholar 

  14. Gozales-Cadavid, N. F. and Campbell, P. N. 1967. Biochem. J. 105, 427–441.

    Google Scholar 

  15. Cohen, H. J. and Fridovich, I. 1971. J. Biol. Chem. 246,359–366.

    Google Scholar 

  16. Folch-Pi, J. and Stoffyn, P. 1972. Ann. N.Y. Acad. Sci. 195,86–107.

    Google Scholar 

  17. Tzagoloff, A., Akai, A. 1972. J. Biol. Chem. 247, 6517–6523.

    Google Scholar 

  18. Stoffyn, P. and Folch-Pi, J. 1971. Biochem. Biophys. Res. Commun. 44, 154–161.

    Google Scholar 

  19. Howe, P. E. 1921. J. Biol. Chem. 49, 109–113.

    Google Scholar 

  20. Omura, T. and Sato, R. 1964. J. Biol. Chem. 239, 2379–2385.

    Google Scholar 

  21. Stripp, B., Hamrick, M. E., and Gillete, J. R. 1972. Biochem. Pharmacol. 21, 747–751.

    Google Scholar 

  22. Matern, S., Frohling, W. and Bock, K. W. 1974. Nauhyn-Schmiedebergs Arch. Pharmacol. 273, 242–247.

    Google Scholar 

  23. Matsumura, A. and Omura, T. 1973. J. Biochem. (Tokyo), 73, 407–416.

    Google Scholar 

  24. Dryan, R., DeBernard, B. and Rabinovitz, M. 1969. J. biol. Chem. 244, 5874–5878.

    Google Scholar 

  25. Raw, I. and Mahler, H. R. 1959. J. Biol. Chem. 234, 1867–1873.

    Google Scholar 

  26. Sottocasa, G. L. Kuylenstierna, B., Ernster, L. and Bergstrand, A. 1967. J. Cell Biol., 32, 415–438.

    Google Scholar 

  27. Coon, M. J., van der Howven, T. Z., Dahl, S. B. and Haugen, D. A. 1978. Methods in Enzymology (Colowick S. P. and Kaplan N. O.), 52, 109–117, Academic Press, N.Y.

  28. Haugen, D. A., Coon, M. J. and Nebert D. W. 1976, J. Biol. Chem., 251, 1817–1827.

    Google Scholar 

  29. Shichi, H., Sugimura, Y. and Funahashi, S. 1964. Biochem. Biophys. Acta, 97, 483–291.

    Google Scholar 

  30. Shichi, H. Sugimura, Y. and Funahashi, S. 1964. biochem, Biophys. Acta, 97, 492–297.

    Google Scholar 

  31. Cattell, K. J., Lindop, C. R., Knight, I. G. and Beechey, R. B. 1971. Biochem. J. 125, 169–177.

    Google Scholar 

  32. Racker, E. and Eytan, E. 1977. J. Biol. Chem., 250, 7533–7534.

    Google Scholar 

  33. Farber, J. L. and Farmar, R. 1973. Biochem. Biophys. Res. commun. 51, 626–630.

    Google Scholar 

  34. Kahl, G. F., Kahl, R., Kumari, K. and Neubert, D. W. 1976. J. Biol. Chem. 251, 5397–5407.

    Google Scholar 

  35. Rothblum, L. I., Devlin, T. M. and Ch'hi, J. J. 1976. Biochem. J. 156, 151–157.

    Google Scholar 

  36. Ch'hi, J. J., Procyk, R. and Devlin, T. M. 1977. Biochem. J. 162, 501–507.

    Google Scholar 

  37. Ernest, M. J., DeLap, L. and Feigelson, P. 1978. J. Biol. Chem. 253, 2895–2897.

    Google Scholar 

  38. Levine, J. H., Nicholson, W. E. and Orth, D. N. 1975. Proc. Nat. Acad. Sci. USA, 72, 2279–2283.

    Google Scholar 

  39. Patterson, D. S. P. 1973. Food Cosmet. Toxicol. 11, 287–293.

    Google Scholar 

  40. Sidransky, H., Verney, E. Murty, C. N., Sarma, D. S. R. and Reid, M. 1977. Chem-Biol. Interactions 18, 69–82.

    Google Scholar 

  41. Reid, I. M., Sarma, S. S. R., and Sidransky, H. 1971. Lab Invest. 25, 141–148.

    Google Scholar 

  42. Murty, C. N. and Sidransky, H. 1972. Biochem. Biophys. Acta 281, 69–78.

    Google Scholar 

  43. John, D. W. and Miller, L. L. 1966. J. Biol. Chem. 241, 4817–4824.

    Google Scholar 

  44. Wilson, S. H., Hill, H. Z. and Hoagland, M. B. 1967. Biochem. J. 103, 567–572.

    Google Scholar 

  45. Tagaki, M. and Ogata, K. 1968. Biochem. Biophys. Res. Commun., 33, 55–61.

    Google Scholar 

  46. Gonzales-Cadavid, N., Orga, J. P. and Gonzales, M., 1971. Biochem. J., 124, 685–694.

    Google Scholar 

  47. Firkin, F. C., and Linnane, A. W. 1969. Exp. Cell Res. 55,68–76.

    Google Scholar 

  48. Hallman, M. 1971. Biochem. Pharmacol. 20, 1797–1809.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sophie Davis Center for Biomedical Education City College, CUNY, New York, USA

    Isaias Raw & Patricia Rockwell

Authors
  1. Isaias Raw
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patricia Rockwell
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This research was supported by grants from the PSC-BHE Research Award Program of The City University of New York, US Public Health Service Grant SO 7 PRO7132-07 and the Alma Toorock Fund for Cancer Research.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Raw, I., Rockwell, P. Effect of a single dose of inducers and inhibitors on the rate of synthesis of cytochromes and reductases in liver organelles. Mol Cell Biochem 28, 7–16 (1979). https://doi.org/10.1007/BF00223355

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00223355

Keywords

Search

Navigation