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Heavy and light forms of some aminoacyl-tRNA synthetases in fraction X, microsomes and cytosol of rabbit liver

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Summary

Aminoacyl-tRNA synthetase activity for alanine, glutamic acid, lysine and phenylalanine was studied in the three subcellular fractions of rabbit liver: fraction X, microsomes and cytosol. From 60 to 80% of the enzyme activities were found in fraction X and microsomes. Fraction X was especially rich in the synthetase activities. By means of gel chromatography, heavy (over 106 daltons) and light (below 480 × 103 daltons) forms of lysyl- and phenylalanyl- but only light ones of alanyl- and glutamyl-tRNA synthetase activities were found in all the subcellular fractions studied. It is concluded that in higher organisms (mammals) all aminoacyl-tRNA synthetases, at least in part, are associated with cell structural constituents.

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Abbreviations

ALA, GLU, LYS, PHE:

alanyl-, glutamyl-, lysyl-, phenylalanyl-tRNA synthetase

PMSF:

phenylmethylsulfonyl fluoride

BSA:

bovine serum albumin

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Authors and Affiliations

  1. Department of Physiological Chemistry, Medical School, Lubartowska 85, 20-123, Lublin, Poland

    Henryk Berbeć, Alicja Paszkowska & Tomasz Borkowski

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  1. Henryk Berbeć
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  2. Alicja Paszkowska
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  3. Tomasz Borkowski
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Berbeć, H., Paszkowska, A. & Borkowski, T. Heavy and light forms of some aminoacyl-tRNA synthetases in fraction X, microsomes and cytosol of rabbit liver. Mol Cell Biochem 62, 149–155 (1984). https://doi.org/10.1007/BF00223305

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  • DOI: https://doi.org/10.1007/BF00223305

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