Summary
In the neuro-intermediate lobe (NIL) of the eel, Anguilla anguilla, a specific formaldehyde-induced fluorescence, indicating a catecholamine (CA) innervation, has been demonstrated in the neural lobe processes. Microspectrofluorimetric analyses and pharmacological treatments indicate noradrenaline or dopamine or both to be responsible for the fluorescence.
The fluorescence in the NIL has displayed a definite tendency toward variation during the adaptation to a white and to a black background. The highest amounts of fluorescence were generally found in animals adapted to a black background, especially when adapted for a rather long period, and in animals recently transferred to a white background. The lowest amounts of fluorescence were generally found in animals adapted to a white background.
This and the result of injections of CA-depleting drugs suggest that the monoaminergic nerves are active when the animal is on a white background, inhibiting the MSH release directly or indirectly or both, or in co-operation with other factors.
Specific green fluorescent structures were also found in other parts of the neural lobe supplying the pars distalis.
In some pharmacologically untreated specimens and in animals treated with CA-depleting drugs, the intermedia cells fluoresced. Microspectrofluorimetric analyses indicated that this fluorophore was not a CA.
We wish to express our sincere thanks to Miss Ingrid Carlsen for excellent technical assistance, Mr. Lajos Erdös for the photography and the technical staff of the Department of Histology in Lund. We are also indepted to Dr. Anders Björklund for valuable discussion and advice.
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References
Baker, B. I.: Sécrétion de l'hormone mélanostimulante par l'hypophyse de poisson “in vitro”. Biol. méd. (Niterói) 56, 351–358 (1967)
Baker, B. I.: The cellular source of melanocyte-stimulating hormone in Anguilla pituitary. Gen. comp. Endocr. 19, 515–521 (1972)
Ball, J. N., Baker, B. I.: The pituitary gland: Anatomy and histophysiology. In: W. S. Hoar and Randall (eds.), Fish physiology, vol. II, p. 1–110. New York and London: Academic press 1969
Ball, J. N., Baker, B. I., Olivereau, M., Peter, R. E.: Investigations on hypothalamic control of adenohypophysical functions in teleost fishes. Gen. comp. Endocr., Suppl. 3, 11–21 (1972)
Baumgarten, H. G., Braak, H.: Catecholamine im Hypothalamus vom Goldfisch (Carassius auratus). Z. Zellforsch. 80, 246–263 (1967)
Bern, H. A., Zambrano, D., Nishioka, R. S.: Comparison of the innervation of the pituitary of two euryhaline teleost fishes, Gillichthys mirabilis and Tilapia mossambica, with special reference to the origin and nature of type “B” fibres. Mem. Soc. Endocr. 19, 817–822 (1971)
Björklund, A.: Monoamine-containing fibres in the pituitary neuro-intermediate lobe of the pig and rat. Z. Zellforsch. 89, 573–589 (1968)
Björklund, A., Ehinger, B., Falck, B.: A method for differentiating dopamine from noradrenaline in tissue sections by microspectrofluorometry. J. Histochem. Cytochem. 16, 263–270 (1968)
Björklund, A., Enemar, A., Falck, B.: Monoamines in the hypothalamo-hypophyseal system of the mouse with special reference to the ontogenetic aspects. Z. Zellforsch. 89, 590–607 (1968)
Björklund, A., Falck, B.: Pituitary monoamines of the cat with special reference to the presence of an unidentified monoamine-like substance in the adenohypophysis. Z. Zellforsch. 93, 254–264 (1969)
Björklund, A., Falck, B., Rosengren, E.: Monoamines in the pituitary gland of the pig. Life Sci. 6, 2103–2110 (1967)
Falck, B., Owman, Ch.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund, Sectio II, No 7, 1–23 (1965)
Follénius, E.: Innervation adrénergique de la métaadénohypophyse de l'Epinoche (Gasterosteus aculeatus L.). Mise en évidence par autoradiographic au microscope électronique. C. R. Acad. Sci. (Paris), Sér. D 267, 1208–1211 (1968)
Follénius, E.: La localisation des terminaisons nerveuses fixant la noradrénaline H3 dans les différents lobes de l'adénohypophyse de l'Epinoche (Gasterosteus aculeatus L.). In: W. Bargmann and B. Scharrer (eds.), Aspects of neuroendocrinology, Vth International Symposium on Neurosecretion, p. 232–244. Berlin-Heidelberg-New York: Springer 1970
Follénius, E.: Action de la 6 OH dopamine sur les mélanophores et sur l'adaptation chromatique chez le poisson téléostéen Gasterosteus aculeatus L. C. R. Acad. Sci. (Paris), Sér. D 272, 733–736 (1971)
Follénius, E.: Cytologic fine de la dégénérescence des fibres aminergiques intrahypophysaires chez le poisson téléostéen Gasterosteus aculeatus après traitement par la 6 hydroxydopamine. Z. Zellforsch. 128, 69–82 (1972)
Fremberg, M., Olivereau, M.: Melanophore responses and intermediate-lobe activity in the eel Anguilla anguilla after injection of 6-OH-dopamine. Acta zool. 54, 231–239 (1973)
Håkansson, R., Sundler, F., Nobin, A., Sjöberg, N.-O., Edvinsson, L., Larsson, L.-I.: Peptides with NH2-terminal tryptophan in the adenohypophysis: A chemical and fluorescence histochemical study. Cell. Tiss. Res. 150, 281–290 (1974)
Holder, F. C.: Arguments expérimentaux de la localisation intragranulaire des hormones hypothalamo-neurohypophysaires chez Anguilla anguilla L. Z. Zellforsch. 140, 315–332 (1973)
Holder, F. C.: Arguments biochimiques et histologiques en faveur du transport et de l'accumulation parallèles des hormones et des grains de neurosécrétion chez l'anguille hypophysectomisée. Z. Zellforsch. 140, 333–355 (1973)
Holmes, R. L., Ball, J. N.: The pituitary gland in teleost fishes. In: The pituitary gland, p. 170–220. London: Cambridge Univ. Press 1974
Honma, S., Honma, Y.: Histochemical demonstration of monoamines in the hypothalamus of the lampreys and ice-goby. Bull. Jap. Soc. Sci. Fish. 36, 125–134 (1970)
Iturriza, F. C.: Monoamines and control of the pars intermedia of the toad pituitary. Gen. comp. Endocr. 6, 19–25 (1966)
Iturriza, F. C.: Monoamines in the neuro-intermediate lobe of the pituitary of the Argentinian eel. Naturwissenschaften 54, 565 (1967)
Iturriza, F. C.: Further evidence for the blocking effect of Catecholamines on the secretion of melanocyte-stimulating hormone in toads. Gen. comp. Endocr. 12, 417–426 (1969)
Khokhar, R.: Experimental evidence of inhibitory control of pars intermedia function and the rate of recovery of function after denervation in the teleost Ictalurus melas. Experientia (Basel) 27, 340–341 (1971)
Knowles, F., Vollrath, L.: Neurosecretory innervation of the pituitary of the eels Anguilla and Conger. I. The structure and the ultrastructure of the neuro-intermediate lobe under normal and experimental conditions. Phil. Trans. B 250, 311–327 (1966)
Leatherland, J.: Histophysiology and innervation of the pituitary gland of the goldfish, Carassius auratus L.: a light and electron microscope investigation. Canad. J. Zool. 50, 835–844 (1972)
Leatherland, J., Dodd, J. M.: Activity of the hypothalamo-neurohypophysial complex of the European eel (Anguilla anguilla L.) assessed by the use of an in situ staining technique and by autoradiography. Gen. comp. Endocr. 13, 45–59 (1969)
L'Hermite, A., Lefranc, G.: Recherches sur les voies monoaminergiques de l'encéphale d'Anguilla vulgaris. Arch. Anat. micr. Morph. exp. 61, 139–152 (1972)
Meurling, P.: Pars intermedia and background adaptation in the skate, Raja radiata. Acta zool. 53, 195–204 (1972)
Neill, R. M.: On the existence of two types of chromatic behaviour in teleostean fishes. J. exp. Biol. 17, 74–95 (1940)
Öztan, N.: The Structure of the hypothalamic neurosecretory cells of Zoarces viviparus L. under conditions of constant dark and light during the reproductive cycle. Z. Zellforsch. 75, 66–82 (1966)
Olivereau, M.: Activité de la pars intermedia de l'hypophyse autotransplantée chez l'Anguille. Z. Zellforsch. 98, 74–87 (1969)
Olivereau, M.: Elaboration d'intermédine par les cellules colorées avec l'hématoxyline au plombe dans la pars intermedia de l'Anguille: preuves nouvelles et contrôle hypothalamique. C. R. Acad. Sci. (Paris), Sér. D 272, 102–105 (1971)
Olivereau, M.: Action de la réserpine chez l'Anguille. II Effet sur la pigmentation et le lobe intermédiaire. Comparaison avec Peffect de l'adaptation sur un fond noir. Z. Anat. Entwickl.-Gesch. 137, 30–46 (1972)
Perks, A. M.: The neurohypophysis. In: W. S. Hoar and D. J. Randall (eds.), Fish physiology, vol. II, p. 111–205. New York and London: Academic Press 1969
Peter, R. E.: Neuroendocrinology of teleosts. Amer. Zool. 13, 743–755 (1973)
Prasada Rao, P. D., Hartwig, H. G.: Monoaminergic tracts of the diencephalon and innervation of the pars intermedia in Rana temporaria. A fluorescence and microspectrofluorimetric study. Cell. Tiss. Res. 151, 1–26 (1974)
Sathyanesan, A. G.: Hypothalamo-hypophyseal neurosecretory system of fishes under some experimental conditions. Gen. comp. Endocr. Suppl. 2, 268–274 (1969)
Schally, A. V., Arimura, A., Kastin, A. J.: Hypothalamic regulatory hormones. Science 179, No 4071, 341–350 (1973)
Schreibman, M. P., Leatherland, J. F., McKeown, B. A.: Functional morphology of the teleost pituitary gland. Amer. Zool. 13, 719–742 (1973)
Terlou, M., Ploemacher, R. E.: The distribution of monoamines in the tel-, diand mesencephalon of Xenopus laevis tadpoles, with special reference to the hypothalamo-hypophysial system. Z. Zellforsch. 137, 521–540 (1973)
Tomatis, M. E., Taleisnik, S.: Influence of reserpine on the pituitary content of melanocytestimulating hormone and on hypothalamic factors which affect its release. J. Endocr. 42, 505–512 (1968)
Urano, A.: Monoamine oxidase in the hypothalamo-hypophysial region of the teleosts, Anguilla japonica and Oryzias latipes. Z. Zellforsch. 114, 83–94 (1971)
Waring, H.: The chromatic behaviour of the eel (Anguilla vulgaris L.) Proc. roy. Soc. B 128, 343–355 (1940)
Waring, H., Landgrebe, F. W.: On chromatic effector speed in Xenopus and Anguilla and the level of melanophore expanding hormone in the eel blood. J. exp. Biol. 18, 80–97 (1941)
Weiss, J.: Saisonale Veränderungen des Enzymmusters und des Neurosekretgehaltes sowie dic Innervation des Nucleus praeopticus der Bachforelle (Salmo trutta fario) unter besonderer Berücksichtigung der hypothalamischen Hydrencephalokrinie. Jahrb. Morphol. Mikrosk. Anat. 115, 444–486 (1970)
Zambrano, D.: Innervation of the teleost pituitary. Gen. comp. Endocr., Suppl. 3, 22–31 (1972)
Zambrano, D., Nishioka, R. S., Bern, H. A.: The innervation of the pituitary gland of teleost fishes. In: Brain-endocrine interaction. Median eminence: structure and function. Intern. Symp. Munich 1971 (Knigge, K. M., Scott, E. E., Weindl, A., eds.), p. 50–65. Basel: Karger 1972
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Supported by grants from the Swedish Natural Science Research Council, the University of Lund, and the Royal Physiographic Society of Lund.
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Fremberg, M., Meurling, P. Catecholamine fluorescence in the pituitary of the eel, Anguilla anguilla, with special reference to its variation during background adaptation. Cell Tissue Res. 157, 53–72 (1975). https://doi.org/10.1007/BF00223230
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DOI: https://doi.org/10.1007/BF00223230