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Diversity of non-histone protein fraction NHCP2 from hamster Kirkman-Robbins hepatoma and liver

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Abstract

Non-histone protein fraction NHCP2 eluted from hydroxyapatite with 100mM phosphate buffer (pH6.8) of undigested, nuclease-sensitive and nuclease-resistant nuclei of hamster Kirkman-Robbins hepatoma and liver was studied by two-dimensional gel electrophoresis and microcomplement fixation test in the presence of antibodies elicited against NHCP2 of examined tissues. The NHCP2 of undigested nuclei as well as from two chromatin fractions with different susceptibility to nuclease of both tissues, besides many common components, showed some differences in their non-histone patterns especially within molecular weights of 17 000–24 000, 36 000–44 000 and 60 000–90 000. Immunological analysis confirmed the high specificity of hepatoma non-histone components of the NHCP2 fraction. However, these components appeared not to be exclusively localized either in nuclease-sensitive or nuclease-resistant part of chromatin of neoplastic tissue.

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Authors and Affiliations

  1. Department of Cytobiochemistry, Institute of Biochemistry, University of Lodz, S. Banacha 12/16, 90-237, Lodz, Poland

    Wanda M. Krajewska, Zofia Kilianska, Anna Lipinska & Leokadia Klyszejko-Stefanowicz

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  1. Wanda M. Krajewska
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  2. Zofia Kilianska
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  3. Anna Lipinska
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  4. Leokadia Klyszejko-Stefanowicz
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Krajewska, W.M., Kilianska, Z., Lipinska, A. et al. Diversity of non-histone protein fraction NHCP2 from hamster Kirkman-Robbins hepatoma and liver. Mol Cell Biochem 83, 37–46 (1988). https://doi.org/10.1007/BF00223196

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  • DOI: https://doi.org/10.1007/BF00223196

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