Abstract
Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite (Acer tulipae), is an important disease of wheat (Triticum aestivum L.) in the North American Great Plains. Resistant varieties have not been developed for two primary reasons. First, useful sources of resistance have not been available, and second, field screening for virus resistance is laborious and beyond the scope of most breeding programs. The first problem may have been overcome by the development of resistance to both the mite and the virus by the introgression of resistance genes from wild relatives of wheat. To help address the second problem, we have developed polymerase chain reaction (PCR) markers linked to the WSMV resistance gene Wsm1. Wsm1 is contained on a translocated segment from Agropyron intermedium. One sequence-tagged-site (STS) primer set (WG232) and one RAPD marker were found to be linked to the translocation containing Wsm1. The diagnostic RAPD band was cloned and sequenced to allow the design of specific PCR primers. The PCR primers should be useful for transferring Wsm1 into locally adapted cultivars.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andersen JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043
Carroll TW, Zaske SK, Brlansky RH (1982) Separation of Montana isolates of wheat streak mosaic virus on Michigan amber wheat. Plant Dis 66:916–918
Chen HB, Martin JM, Lavin M, Talbert LE (1994) Genetic diversity of hard red spring wheat based on STS-PCR molecular markers. Crop Sci 34:1628–1632
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Devos KM, Gale MD (1992) The use of random amplified polymorphic DNA in wheat. Theor Appl Genet 84:567–572
Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS (1991) Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization. Theor Appl Genet 81:381–389
Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374
Gill BS, Friebe B, Wilson DL, Martin TJ, Cox TS (1995) Registration of KS93WGRC27 wheat streak mosaic virus-resistant hard red winter wheat germ plasm. Crop Sci 35:1236–1237
Harvey TL, Martin TJ (1988) Sticky tape method to measure cultivar effects on wheat curl mite (Acari: Eriophyidae) populations in wheat spikes. J Econ Entomol 81:731–734
Lassner MW, Peterson P, Yoder JI (1989) Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Mol Biol Rep 7:116–128
Martin TJ (1978) Procedures for evaluating wheat streak mosaic virus resistance. Plant Dis Rep 62:1062–1066
Nelson JC, Van Deynz AE, Autrique E, Sorrells ME, Lu YH, Negre S, Bernard M, Leroy P (1995) Molecular mapping of wheat. Hmoeologous group 3. Genome 38:525–533
Sears ER (1954) The aneuploids of common wheat. Res Bull Mo Agric Exp Sta 472
Slykhuis JT (1967) Methods for experimenting with mite transmission of plant viruses. Methods Virol 1:347–368
Stoddard SL, Lommel SA, Gill BS (1987) Evaluation of wheat germ plasm for resistance to wheat streak mosaic virus by symptomatology, ELISA, and slot-blot hybridization. Plant Dis 71:714–719
Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of sequence-tagged-site PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794
Talbert LE, Blake NK, Storlie EW, Lavin M (1995) Variability in wheat based on low-copy DNA sequence comparisons. Genome 38:951–957
Tragoonrung S, Kanazin V, Hayes PM, Blake TK (1992) Sequence-tagged-site-facilitated PCR for barley genome mapping. Theor Appl Genet 84:1002–1008
Weiss MV (1987) Compendium of wheat diseases, 2nd edn. APS Press, St. Paul, Minnesota
Williams JGK, Kubelik ARK, Livak JL, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by random primers are useful as genetic markers. Nuclei Acids Res 18:6531–6535
Author information
Authors and Affiliations
Additional information
Communicated by G. E. Hart
This is Journal Series No. J-4041 of the Montana Agricultural Experiment Station
Rights and permissions
About this article
Cite this article
Talbert, L.E., Bruckner, P.L., Smith, L.Y. et al. Development of PCR markers linked to resistance to wheat streak mosaic virus in wheat. Theoret. Appl. Genetics 93, 463–467 (1996). https://doi.org/10.1007/BF00223191
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223191