Abstract
Genetic variation was assessed among cultivated and wild hop, Humulus lupulus, by restriction fragment length polymorphisms (RFLPs) of the ribosomal RNA genes (rDNA). Two rDNA length variants of 10.3 and 9.3 kbp represented by three phenotypes designated A, B and C were detected with XhoI. Restriction-site mapping showed that hop rDNA is structurally similar to those of most higher plants. A high level of homogeneity existed in rDNA repeat lengths among the diverse hop genotypes. Generally, phenotype A was predominant in wild and cultivated European and Asian genotypes; phenotype B in North American cultivars; while phenotype C was present only in native North American hop, providing a potential molecular marker for the identification of this germ plasm. The rDNA data provided genetic evidence for the separation of native and cultivated American genotypes and supports the hypothesis that North American hop cultivars are of hybrid origin from European and native American genotypes. The segregation of rDNA phenotypes in four F1 families suggests that a single locus with two co-dominant alleles controls genetic variability for rDNA variants in hop.
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Allard RW, Saghai-Maroof MA, Zhang Q, Jorgensen RA (1990) Genetic and molecular organization of ribosomal DNA (rDNA) variants in wild and cultivated barley. Genetics 126:743–751
Appels R, Dvorak J (1982) The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. Theor Appl Genet 63:337–348
Appels R, Honeycutt RL (1986) rDNA: evolution over a billion years. In: Dutta SK (ed) DNA systematics. CRC Press, Boca Raton, Florida, pp 81–135
Appels R, McIntyre CL, Clarke BC (1986) Alien chromatin in wheat: ribosomal DNA spacer probes for detecting specific nucleolar organizer region loci introduced into wheat. Can J Genet Cytol 28:665–672
Beckman JS, Soller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Apple Genet 67:35–43
Besse P, Seguin M, Lebrun P, Lanaud C (1993) Ribosomal DNA variations in wild and cultivatied rubber tree (Hevea brasiliensis). Genome 36:1049–1057
Borisjuk N, Borisjuk L, Petjuch G, Hemleben V (1994) Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome 37:271–279
Capossela A, Silander JA Jr, Jansen RK, Bergen B, Talbot DR (1992) Nuclear ribosomal DNA variation among ramets and genets of white clover. Evolution 46:1240–1247
Cluster PD, Jorgensen RA, Bernatsky R, Hakim-Elahi A, Allard RW (1984) The genetics and geographical distribution of ribosomal DNA spacer-length variation in the wild oat, Avena barbata. Genetics 107:s21
Delseny M, McGrath JM, This P, Chevre AM, Quiros CF (1990) Ribosomal RNA genes in diploid and amphidiploid Brassica and related species: organization, polymorphism, and evolution. Genome 33:733–744
Doyle JJ, Beachy RN (1985) Ribosomal gene variation in soybean (Glycine) and its relatives. Theor Appl Genet 70:369–376
Doyle JJ, Soltis DE, Soltis PS (1985) An intergeneric hybrid in the Saxifragaceae: evidence from the ribosomal RNA genes. Am J Bot 72:1388–1391
Ellis THN, Davies DR, Castleton JA, Bedford ID (1984) The organization and inheritance of rDNA length variants in peas. Chromosoma 91:74–81
Flavell RB, O'Dell M, Sharp P, Nevo E, Beiles A (1986) Variation in the intergenic spacer of ribosomal DNA of wild wheat, Triticum dicoccoides, in Israel. Mol Biol Evol 3:547–558
Govindaraju D, Lewis P, Cullis C (1992) Phylogenetic analysis of pines using ribosomal DNA restriction fragment length polymorphisms. Pl Syst Evol 179:141–153
Jellen EN, Phillips RL, Rines HW (1994) Chromosomal localization and polymorphisms of ribosomal DNA in oat (Avena spp.). Genome 37:23–32
Karvonen P, Savolainen O (1993) Variation and inheritance of ribosomal DNA in Pinus sylvestris. Heredity 71:614–622
Kim K-J, Mabry TJ (1990) Phylogenetic and evolutionary implications of nuclear ribosomal DNA variation in dwarf dandelion (Kriga, Lactuceae, Asteraceae). Pl Syst Evol 177:53–69
Kim WK, Innes RL, Kerber ER (1992) Ribosomal DNA repeat unit polymorphism in six Aegilops species. Genome 35:510–515
Lamppa GK, Honda S, Bendich AJ (1984) The relationship between ribosomal repeat length and genome size in Vicia. Chromosoma 89:1–7
Laurent V, Risterucci A-M, Lanaud C (1993) Variability for nuclear ribosomal genes within Theobroma cacao. Heredity 71:96–103
May CE, Apples R (1987) Variability and genetics of spacer DNA sequences between the ribosomal-RNA genes of hexaploid wheat (Triticum aestivum). Theor Appl Genet 74:617–624
Neve RA (1991) Hops. Chapman and Hall, London
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica. Theor Appl Genet 65:181–189
Pillay M, Kenny ST (1994) Chloroplast DNA differences between cultivated hop, Humulus lupulus, and the related species H.japonicus. Theor Appl Genet 89:372–378
Pillay M, Kenny ST (1996) Random amplified polymorphic DNA (RAPD) markers in hop, Humulus lupulus:level of genetic variability and segragation in F1 progeny. Theor Appl Genet 92:334–339
Polans, NO, Weeden NF, Thompson WF (1986) Distribution, inheritance and linkage relationship of ribosomal DNA spacer length variants in pea. Theor Appl Genet 72:289–295
Rafalski JA, Wievioroswki M, Soll D (1983) Organization of ribosomal DNA in yellow (Lupinus luteus) and sequence of the 5.8S RNA gene. Fed Eur Biochem Soc Lett 152:241–246
Rieseberg LH (1991) Homoploid reticulate evolution in Helianthus (Asteraceae): evidence from ribosomal genes. Am J Bot 78:1218–1237
Rocheford TR (1994) Change in ribosomal DNA intergenic spacer-length composition in maize recurrent selection populations. 1. Analysis of BS13, BSSS, and BSCBI. Theor Appl Genet 88:541–547
Rocheford TR, Osterman JC, Gardner CO (1990) Variation in the ribosomal DNA intergenic spacer of a maize population mass-selected for high grain yield. Theor Appl Genet 79:793–800
Rogers SO, Bendich AJ (1987) Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol Biol 9:509–520
Sano Y (1989) Molecular cloning of ribosomal RNA genes from the two cultivated species, Oryza sativa and O. Glaberrima. Annu Rep Natl Inst Genet Mishima, Japan, 39:112–113
Saghai-Maroof MA, Soliman KM, Jorgenesen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Schaal BA, Leverich WJ, Nieto-Sotelo J (1987) Ribosomal DNA variation in the native plant Phlox divaricata. Mol Biol Evol 4:611–621
Simcox KD, McMullen MD, Louie R (1995) Co-segregation of the maize dwarf mosaic virus gene, Mdm1, with the nucleolar region in maize. Theor Appl Genet 90:341–346
Small E (1978) A numerical and nomenclatural analysis of morphogeographic taxa of Humulus. Systematic Bot 3:37–76
Small E (1980) The relationships of hop cultivars and wild variants of Humulus lupulus. Can J Bot 58:676–686
Snape JW, Flavell RB, O'Dell M, Hughes WG, Payne PI (1985) Intrachromosomal mapping of the nucleolar organizer region relative to three marker loci on the chromosome 1B of wheat. Theor Appl Genet 69:263–270
Sytsma KJ, Schaal BA (1990) Ribosomal DNA variation within and among individuals of Lisianthius (Gentianaceae) populations. Pl Syst Evol 170:97–106
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technol 7:257–264
Tremouseaygue D, Grellet F, Delseny M, Delourme R, Renard M (1988) The large spacer of a nuclear ribosomal RNA gene from radish: organization and use as a probe in rape seed breeding. Theor Appl Genet 75:298–304
Yakura K, Kato A, Tanifuji S (1984) Length heterogeneity in the large spacer of Vicia faba rDNA is due to the differing number of a 325-bp repetitive sequence elements. Mol Gen Genet 193:400–405
Zimmer EA, Jupe ER, Walbot V (1988) Ribosomal gene structure, variation, and its inheritance in maize and its ancestors. Genetics 120:1125–1136
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Communicated by H.F. Linskens
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Pillay, M., Kenny, S.T. Structure and inheritance of ribosomal DNA variants in cultivated and wild hop, Humulus lupulus L.. Theoret. Appl. Genetics 93, 333–340 (1996). https://doi.org/10.1007/BF00223173
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DOI: https://doi.org/10.1007/BF00223173