Summary
The effects of chronic administration of ACTH and dexamethasone on the morphology of mitochondria in zona glomerulosa cells of the rat adrenal cortex were investigated by Stereological techniques. It was found that the volume of the mitochondrial compartment as well as the surface of the outer and inner mitochondrial membranes were significantly increased or decreased in relation to the number of days of ACTHor dexamethasone-treatment. In ACTH-administered rats, the average volume of individual mitochondria decreased significantly up to the 6th day of treatment and then showed a conspicuous increase from the 6th to the 15th day, whereas in dexamethasone-administered animals this parameter, after a small increase during the first 6 days of treatment, displayed a significant decrease. The number of mitochondria per cell showed a dramatic increase during the first 6 days of treatment with ACTH and continued to increase, but only slightly, with the subsequent treatment. In contrast, this parameter showed a parabolic decrease as a function of the duration of treatment in animals receiving dexamethasone. In the light of evidence showing that dexamethasone blocks ACTH-release, these findings are discussed and interpreted to indicate that ACTH is involved in the maintenance and stimulation of the growth and proliferative activity of mitochondria in rat adrenal zona glomerulosa.
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Ashwell, M., Work, T.S.: The biogenesis of mitochondria. Ann. Rev. Biochem. 39, 251–290 (1970)
Bergeron, M., Droz, B.: Protein renewal in mitochondria as revealed by electron microscopic autoradiography. J. Ultrastruct. Res. 26, 17–30 (1969)
Canick, J.A., Purvis, J.L.: The maintenance of mitochondrial size in the rat adrenal zona fasciculata by ACTH. Exp. molec. Path. 16, 79–93 (1972)
Canick, J.A., Villee, D.B.: The effect of adrenocorticotrophin on protein degradation in rat adrenal gland and liver. Biochem. J. 144, 397–403 (1974)
Guttes, E.W., Hanawalt, P.C., Guttes, S.: Mitochondrial DNA synthesis and the mitotic cycle in Physarum polycephalum. Biochim. biophys. Acta (Amst.) 142, 181–194 (1967)
Ichii, S., Murakami, N., Izawa, M.: Effect of ACTH and dexamethasone on the rate of protein and RNA degradation in rat adrenal glands. Endocr. jap. 21, 465–469 (1974)
Mazzocchi, G., Robba, C., Belloni, A.S., Rigotti, P., Gambino, A.M., Nussdorfer, G.G.: Investigations on the turnover of adrenocortical mitochondria. IV. A stereological study of the effect of chronic treatment with ACTH on the size and number of rat zona reticularis mitochondria. Cell Tiss. Res. 168, 1–9 (1976a)
Mazzocchi, G., Robba, C., Neri, G., Gottardo, G., Nussdorfer, G.G.: Investigations on the turnover of adrenocortical mitochondria. V. An autoradiographic study of the radioactivity decay in the mitochondrial compartment from the adrenal cortex of 3H-thymidine-injected rats. Cell Tiss. Res. 172, 149–156 (1976b)
Nussdorfer, G.G.: Effects of corticosteroid-hormones on the smooth endoplasmic reticulum of rat adrenocortical cells. Z. Zellforsch. 106, 143–154 (1970)
Nussdorfer, G.G., Mazzocchi, G.: Effect of 3′∶5′-cyclic AMP on nuclear and mitochondrial RNA synthesis of rat adrenocortical cells. Acta endocr. (Kbh.) 70, 81–88 (1972)
Nussdorfer, G.G., Mazzocchi, G.: Effect of adrenocorticotrophin on 3H-uridine incorporation into glomerulosal cells of hypophysectomized rat adrenals. J. Endocr. 63, 581–582 (1974)
Nussdorfer, G.G., Mazzocchi, G., Gottardo, G.: Investigations on the turnover of adrenocortical mitochondria. II. Effect of chronic treatment with ACTH on the 3H-thymidine incorporation into rat zona fasciculata mitochondria. Cell Tiss. Res. 151, 281–292 (1974a)
Nussdorfer, G.G., Mazzocchi, G., Rebonato, L.: Long-term trophic effect of ACTH on rat adrenocortical cells. An ultrastructural, morphometric and autoradiographic study. Z. Zellforsch. 115, 30–45 (1971)
Nussdorfer, G.G., Mazzocchi, G., Rebuffat, P.: An ultrastructural stereologic study of the effects of ACTH and adenosine 3′,5′-cyclic monophosphate on the zona glomerulosa of rat adrenal cortex. Endocrinology 92, 141–151 (1973)
Nussdorfer, G.G., Mazzocchi, G., Rebuffat, P.: An ultrastructural stereologic study of the effects of 3′,5′-cyclic nucleotides on the zona glomerulosa of rat adrenal cortex. Endocrinology 94, 602–608 (1974b)
Nussdorfer, G.G., Mazzocchi, G., Rebuffat, P., Belloni, A.S., Gottardo, G., Gambino, A.M.: Investigations of the turnover of adrenocortical mitochondria. III. Stereologic and autoradiographic observations on the effect of chronic treatment with dexamethasone on the rat zona fasciculata mitochondria. Cell Tiss. Res. 163, 273–290 (1975)
Nussdorfer, G.G., Mazzocchi, G., Robba, C., Belloni, A.S., Gambino, A.M.: Investigations on the turnover of adrenocortical mitochondria. VI. An autoradiographic study of the effect of ACTH on the radioactivity decay in the mitochondrial compartment from the zona fasciculata of 3Hthymidine-injected rats. Anat. Rec., in press (1977)
Nussdorfer, G.G., Rebuffat, P., Mazzocchi, G., Belloni, A.S., Meneghelli, V.: Investigations on adrenocortical mitochondria turnover. I. Effect of chronic treatment with ACTH on the size and number of rat zona fasciculata mitochondria. Cell Tiss. Res. 150, 79–94 (1974c)
Robba, C., Mazzocchi, G., Belloni, A.S., Nussdorfer, G.G.: Investigations on the turnover of adrenocortical mitochondria. VII. Effects of ACTH on the half-life of mitochondria from the zona reticularis of the rat adrenal cortex. Experientia (Basel) 32, 1611–1612 (1976)
Svoboda, D., Grady, H., Higginson, J.: The effects of chronic protein deficiency in rats. II. Biochemical and ultrastructural changes. Lab. Invest. 15, 731–749 (1966)
Tamaoki, B.I.: Stereoidogenesis and cell structure. Biochemical pursuit of sites of steroid biosynthesis. J. Steroid Biochem. 4, 89–118 (1973)
Tandler, B., Erlandson, R.A., Smith, A.L., Wynder, E.L.: Riboflavin and mouse hepatic cell structure and function. II. Division of mitochondria during recovery from simple deficiency. J. Cell Biol. 41, 477–493 (1969)
Tandler, B., Erlandson, R.A., Wynder, E.L.: Riboflavin and mouse hepatic cell structure and function. I. Ultrastructural alteration in simple deficiency. Am. J. Path. 52, 69–78 (1968)
Vanek, R.: Pouvoir freinateur de divers cortico-stéroïdes de synthèse sur la sécrétion d'ACTH. Etude comparative. Ann. Endocr. (Paris) 25, 799–808 (1965)
Weibel, E.R.: Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol. 26, 235–302 (1969)
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The authors wish to thank Prof. R. Mitchell for his critical reading of the manuscript and Miss A. Coi and Mr. G. Gottardo for their excellent technical assistance
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Mazzocchi, G., Robba, C., Rebuffat, P. et al. Investigations on the turnover of adrenocortical mitochondria. Cell Tissue Res. 181, 287–298 (1977). https://doi.org/10.1007/BF00223104
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DOI: https://doi.org/10.1007/BF00223104