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Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns

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Abstract

Several techniques of DNA analysis were applied to identify chrysanthemum cultivars. Unrelated cultivars could be distinguished by using RAPDs (random amplified polymorphic DNAs), inter-SSR (simple sequence repeat) PCR (polymerase chain reaction), hybridization-based DNA fingerprinting, as well as RFLPs (restriction fragment length polymorphisms). Cultivars with different flower colours and belonging to one family, i.e. vegetatively derived from 1 cultivar, appeared to have the same DNA fragment patterns, whichever technique was applied. The absence of polymorphisms between different accessions of the same cultivar indicated a high stability of the observed patterns.

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Author notes

  1. K. Wolff

    Present address: School of Biological and Medical Sciences, University of St. Andrews, Sir Harold Mitchell Building, KY16 9TH, St. Andrews, Fife, Scotland

Authors and Affiliations

  1. Institute of Evolutionary and Ecological Sciences, University of Leiden, PO Box 9516, 2300, RA Leiden, the Netherlands

    K. Wolff

  2. TNO Nutrition and Food Research, PO Box 360, NL-3700, AJ Zeist, The Netherlands

    K. Wolff & H. Hofstra

  3. Service de Génétique Médicale, Centre de Recherche, Hôpital SteJustine, Université de Montréal, H3T 1C5, Quebec, Canada

    E. Zietkiewicz

Authors
  1. K. Wolff
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  2. E. Zietkiewicz
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  3. H. Hofstra
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Communicated by P. M. A. Tigerstedt

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Wolff, K., Zietkiewicz, E. & Hofstra, H. Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns. Theoret. Appl. Genetics 91, 439–447 (1995). https://doi.org/10.1007/BF00222971

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  • DOI: https://doi.org/10.1007/BF00222971

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