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Development of a consensus linkage RFLP map of cultivated sunflower (Helianthus annuus L.)

Theoretical and Applied Genetics volume 90, pages 1079–1086 (1995)Cite this article

Abstract

This paper provides the first description of a consensus map of the cultivated sunflower genome (Helianthus annuus L., n=17 chromosomes), based on RFLP. A total of 180 probe-enzyme combinations were mapped on at least one of five segregating progenies (three F2 and two BC1 populations), revealing 237 loci that did not show any distortion of segregation. The consensus linkage map obtained with these loci covers 1150 cM and consists of 16 linkage groups of more than 20 cM, 7 groups of less than 20 cM and 18 unlinked loci. The mean distance between loci is 7 cM, but in some regions intervals of 20 cM remain. Genotypic and gametic segregation distortions affect about 7% of loci. It was found that 25% of the probes mapped using several different restriction enzymes or that on different progenies they revealed 2 or more loci.

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Author information

Author notes

  1. L. Gentzbittel

    Present address: G.I.E Cartisol, 4 rue Ledru, 63038, Clermont-Ferrand Cedex, France

  2. Y.-X. Zhang

    Present address: GIP GEVES, Unité expérimentale de la Minière, 78285, Guyancourt Cedex, France

Affiliations

  1. G.I.E. Cartisol, 12 avenue George V, 75008, Paris, France

    L. Gentzbittel

  2. INRA, Station de Génétique et d'Amélioration des Plantes, Domaine de Crouelle, 02, Clermont-Ferrand Cedex, France

    F. Vear

  3. ENSA-INRA SGAP, 2 place P. Viala, 1, Montpellier Cedex, France

    A. Bervillé

  4. Unité associée Université Biaise Pascal-Clermont II-INRA, Organisation et Variabilité des Genomes Végétaux, 4 rue Ledru, 63038, Clermont-Ferrand, Cedex, France

    Y.-X. Zhang & P. Nicolas

Authors
  1. L. Gentzbittel
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  2. F. Vear
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  3. Y.-X. Zhang
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  4. A. Bervillé
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  5. P. Nicolas
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Communicated by J.W. Snape

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Gentzbittel, L., Vear, F., Zhang, YX. et al. Development of a consensus linkage RFLP map of cultivated sunflower (Helianthus annuus L.). Theoret. Appl. Genetics 90, 1079–1086 (1995). https://doi.org/10.1007/BF00222925

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  • DOI: https://doi.org/10.1007/BF00222925

Key word

  • RFLP
  • Helianthus annuus
  • Linkage map
  • Consensus map
  • CDNA