Summary
This paper describes the use of oligonucleotide probes to reveal highly polymorphic DNA regions in pomato. With a (GATA)4 probe the level of polymorphism detected is high enough to identify all 15 tomato cultivars used in this study. Individual plants of one cultivar all showed the same cultivar-specific DNA-finger-print. In an F2-population of self-fertilized cv. Sonatine, GATA-containing loci segregated in a Mendelian (3∶1) fashion. Experiments with in-vitro propagated plant material showed that the DNA-fingerprints are not affected by tissue-culture procedures. This indicates that changes in the genetic integrity, which often accompany in-vitro propagation (somaclonal variation), are not extended to the DNA detected with the (GATA)4 probe. The relative high stability and the Mendelian segregation of (GATA)4-derived DNA-fingerprints make them ideally suited for identification of tomato cultivars.
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Communicated by F. Salamini
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Vosman, B., Arens, P., Rus-Kortekaas, W. et al. Identification of highly polymorphic DNA regions in tomato. Theoret. Appl. Genetics 85, 239–244 (1992). https://doi.org/10.1007/BF00222865
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DOI: https://doi.org/10.1007/BF00222865