Summary
Various auxin-resistant Nicotiana plumbaginifolia mutants have already been isolated, including 1217 which shows cross-resistance to paclobutrazol. Recently, a cytokinin-resistant mutant, CKR1, has been characterized and has been shown to be affected in abscisic acid (ABA) biosynthesis. We have isolated a new mutant, Esg152, which was selected on the basis of its early germination. In each of these mutants, resistance is due to a recessive nuclear mutation at a single locus. Complementation analysis indicated that mutants I217, CKR1 and Esg152 belong to the same complementation group. They have a similar phenotype, which includes a reduction in seed dormancy and an increased tendency to wilt. These mutants display an increased auxin tolerance and enhanced root formation when leaf or hypocotyl sections are cultivated on auxin. By immunoenzymatic methods, we show that the endogenous levels of ABA are significantly lower than in the wild-type. We have assigned the symbol aba1 to the recessive alleles of the locus affected in the three mutants. The complexity of hormonal interactions is discussed briefly emerging from a consideration of this class of mutants.
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References
Bitoun R, Rousselin P, Caboche M (1990) A pleiotropic mutation results in cross-resistance to auxin, abscisic acid and paclobutrazol. Mol Gen Genet 220:234–239
Blonstein AD, Parry AD, Horgan R, King PJ (1991 a) A cytokinin-resistant mutant of Nicotiana plumbaginifolia is wilty. Planta 183:244–250
Blonstein AD, Stirnberg P, King PJ (1991 b) Mutants of Nicotiana plumbaginifolia with specific resistance to auxin. Mol Gen Genet 228:361–371
Bourgin JP, Chupeau Y, Missonier C (1979) Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol Plant 45:288–292
Davies WJ, Metcalfe J, Lodge TA, Da Costa AR (1986) Plant growth substances and regulation of growth under drought. Aust J Plant Physiol 13:105–125
Duckham SC, Taylor IB, Linforth RST, Al-Naieb RJ, Marples BA, Bowman WR (1989) The metabolism of cis ABA-aldehyde by the wilty mutants of potato, pea and Arabidopsis thaliana. J Exp Botany 40:901–905
Gabard J, Marion-Poll A, Chérel I, Meyer C, Müller A, Caboche M (1987) Isolation and characterization of Nicotiana plumbaginifolia nitrate reductase-deficient mutants: genetic and biochemical analysis of the NIA complementation group. Mol Gen Genet 209:596–606
Imber D, Tal M (1970) Phenotypic reversion of flacca, a wilty mutant of tomato, by abscisic acid. Science 169:592–593
Jullien M, Lesueur D, Laloue M, Caboche M (1991) Isolation and preliminary characterization of cytokinin-resistant mutants of Nicotiana plumbaginifolia. In: Kaminek M, Mok DWS, Zazimalova E (eds) Physiology and Biochemistry of Cytokinins in plants. SPB Academic Publishing bv, The Hague, The Netherland, pp 157–162
Koornneef M, Jorna ML, Brinckhorst-van der Swan DLC, Karssen CM (1982) The isolation of abscisic acid (ABA)-deficient mutants by selection of induced revertants in non-germinating gibberellin-sensitive lines of Arabidopsis thaliana (L.) Heynh. Theor Appl Genet 61:385–393
Koornneef M, Reuling G, Karssen CM (1984) The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol Plant 61:377–383
Maldiney R, Leroux B, Sabbagh I, Sotta B, Sossountzov L, Miginiac E (1986) A biotin-avidin-based enzyme immunoassay to quantify three phytohormones: auxin, abscisic acid and zeatin-riboside. J Immunol Methods 90:151–158
McCarty DR, Carson CB, Stimard PS, Robertson DS (1989) Molecular analysis of viviparous-1; an abscisic acid-insensitive mutant of maize. Plant Cell 1:523–532
Muller JF, Missonier C, Caboche M (1983) Low density growth of cells derived from Nicotiana and Petunia protoplasts: influence of the source of protoplasts and comparison of the growth-promoting activity of various auxins. Physiol Plant 57:35–41
Parry AD, Blonstein AD, Babiano MJ, King PJ, Horgan R (1991) Abscisic acid metabolism in a wilty mutant of Nicotiana plumbaginifolia. Planta 183:237–243
Pelese F, Megnegneau B, Sotta B, Sossountzov L, Caboche M, Miginiac E (1989) Hormonal characterization of a nonrooting naphtalene-acetic acid tolerant tobacco mutant by an immunoenzymic method. Plant Physiol 89:86–93
Pelsy F, Kronenberger J, Pollien J-M, Caboche M (1991) M2 seed screening for nitrate reductase deficiency in Nicotiana plumbaginifolia. Plant Sci 76:109–114
Quarrie SA (1982) Droopy: a wilty mutant of potato deficient in abscisic acid. Plant Cell Environ 5:23–26
Quarrie SA (1987), Use of genotypes differing in endogenous abscisic acid levels in studies of physiology and development. In: Hoad GV, Lenton JR, Jackson MB, Atkin RK (eds) Hormonal action in plant development. Butterworths, London, UK, pp 89–105
Reid JB (1990) Phytohormone mutants in plant research. J Plant Growth Regul 9:97–111
Rock CD, Zeevaart JAD (1991) The aba mutant Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis. Proc Natl Acad Sci USA 88:7496–7499
Rock CD, Heath TG, Gage DA, Zeevaart JAD (1991) Abscisic alcohol is an intermediate in abscisic acid biosyntheis in a shunt pathway from abscisic aldehyde. Plant Physiol 97:670–676
Rousselin P, Lepingle A, Faure JD, Bitoun R, Caboche M (1990) Ethanol-resistant mutants of Nicotiana plumbaginifolia are deficient in the expression of pollen and seed alcohol dehydrogenase activity. Mol Gen Genet 222:409–415
Tal M, Nevo Y (1973) Abnormal stomatal behavior and root resistance, and hormonal imbalance in three wilty mutants of tomato. Biochem Genet 8:291–300
Tal M, Imber D, Erez A, Epstein E (1979) Abnormal stomatal behavior and hormonal imbalance in flacca, a wilty mutant of tomato. V. Effect of abscisic acid on indolacetic acid metabolism and ethylene evolution. Plant Physiol 63:1044–1048
Taylor IB, Linforth RST, Al-Naieb RJ, Bowman WR, Marples BA (1988) The wilty tomato mutants flacca and sitiens are impaired in the oxidation of ABA-aldehyde to ABA. Plant Cell Environ 11:739–745
Wilson AK, Pickett FB, Turner JC, Estelle M (1990) A dominant mutation in Arabidopsis confers resistance to auxin, ethylene and abscisic acid. Mol Gen Genet 222:377–383
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Rousselin, P., Kraepiel, Y., Maldiney, R. et al. Characterization of three hormone mutants of Nicotiana plumbaginifolia: evidence for a common ABA deficiency. Theoret. Appl. Genetics 85, 213–221 (1992). https://doi.org/10.1007/BF00222862
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DOI: https://doi.org/10.1007/BF00222862