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Variation in mitochondrial translation products in fertile and cytoplasmic male-sterile sugar beets

Theoretical and Applied Genetics volume 85, pages 139–145 (1992)Cite this article

Summary

Intact and functional mitochondria were isolated from sugar beet plants (Beta vulgaris L.) containing normal fertile (F) or cytoplasmic male-sterile (S1–S4) cytoplasms. Incorporation of 35S-methionine by mitochondria isolated from both roots and leaves showed approximately 20 major and ten minor translation products. Comparison of the polypeptide synthesis patterns produced by leaf mitochondria from fertile plants of three different species within the genus Beta revealed several taxonomically related differences. Contrary to this, the patterns of polypeptides synthesized by mitochondria from roots and leaves of sugar beet plants containing the F and S1–S4 cytoplasms were very similar; in the S1 and S2 cytoplasms no qualitative, and only a few quantitative, differences from the F cytoplasm were observed. Thus, in these cases, cytoplasmic male sterility in sugar beet is not correlated with the constitutive expression of variant polypeptides. In the S3 cytoplasm, however, an additional 6 kDa polypeptide was synthesized and in the S4 cytoplasm an additional 10 kDa polypeptide was observed when compared with the F cytoplasm. The expression of cytoplasmic male sterility in sugar beet may be associated with these variant polypeptides. The mitochondrial polypeptides synthesized were identical in plants with different nuclear backgrounds but with identical S1 cytoplasms. Mitochondria from plants with variants of the S4 cytoplasm in the same nuclear genotype also showed identical patterns of polypeptide synthesis, including the synthesis of the 10 kDa S4-specific polypeptide. Pulse-chase experiments did not affect the synthesis of this polypeptide.

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Affiliations

  1. Department of Genetics, Lund University, Sölvegatan 29, S-223 62, Lund, Sweden

    Christer Halldén

  2. Department of Plant Biochemistry, Lund University, Box 7007, S-22007, Lund, Sweden

    Christina Lind

  3. Department of Plant Physiology, Lund University, Box 7007, S-22007, Lund, Sweden

    Ian M. Møller

Authors
  1. Christer Halldén
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  2. Christina Lind
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  3. Ian M. Møller
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Communicated by D.R. Pring

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Halldén, C., Lind, C. & Møller, I.M. Variation in mitochondrial translation products in fertile and cytoplasmic male-sterile sugar beets. Theoret. Appl. Genetics 85, 139–145 (1992). https://doi.org/10.1007/BF00222851

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  • DOI: https://doi.org/10.1007/BF00222851

Key words

  • Beta vulgaris
  • Cytoplasmic male sterility
  • 35S-methionine incorporation
  • Mitochondria
  • Sugar beet