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A technique for maintaining explants in contact with solid medium in petri dishes

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Biotechnology Techniques

Summary

The objective of this study was to develop a system to maintain orchardgrass (Dactylis glomerata L.) leaf segments in contact with solid medium in petri dishes during different orientation of the dishes. To ensure contact with the medium, leaf segments were overlaid with 1800 μm Teflon™ mesh. This was secured with a polypropylene ring which fitted between the petri dish lid and the mesh. This procedure did not affect the somatic embryogenesis response. A significant difference (P=0.05) in increased ethylene accumulation from overlaid segments was recorded on day 5.

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Author notes

  1. Z. Tomaszewski Jr.

    Present address: Department of Tissue Culture, Plant Breeding and Acclimatization Institute, 05-870, Blonie, Poland

Authors and Affiliations

  1. Department of Plant and Soil Science, The University of Tennessee, 37901-1071, Knoxville, TN, USA

    Z. Tomaszewski Jr., A. I. Kuklin & B. V. Conger

Authors
  1. Z. Tomaszewski Jr.
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  2. A. I. Kuklin
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  3. B. V. Conger
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Tomaszewski, Z., Kuklin, A.I. & Conger, B.V. A technique for maintaining explants in contact with solid medium in petri dishes. Biotechnol Tech 8, 467–472 (1994). https://doi.org/10.1007/BF00222837

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  • DOI: https://doi.org/10.1007/BF00222837

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