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Further immunofluorescence-microscopic evidence for myosin in various peripheral nerves

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Summary

An indirect immunofluorescence microscopic technique using antibodies from rabbits against highly purified myosin from chicken gizzard was applied to various peripheral nerves (cranial nerves V, VII, X). Myosinspecific immunoreactivity was found in the axoplasm, in Schwann cells, in the perineural sheath and in vascular walls.

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References

  • Berl, S., Puszkin, S., Nicklas, W.J.: Actomyosin-like protein in brain. Science 179, 441–445 (1972)

    Google Scholar 

  • Bray, D.: Model for membrane movements in the neural growth cone. Nature (Lond.) 244, 93–96 (1973)

    Google Scholar 

  • Burridge, K., Phillips, J.H.: Association of actin and myosin with secretory granule membranes. Nature (Lond.) 254, 256–259 (1975)

    Google Scholar 

  • Chang, C.-M., Goldman, R.D.: The localization of actin-like fibers in cultured neuroblastoma cells as revealed by heavy meromyosin binding. J. Cell Biol. 57, 867–874 (1973)

    Google Scholar 

  • Creutz, C.E.: Isolation, characterization and localization of bovine adrenal medullary myosin. Cell Tiss. Res. 178, 17–38 (1977)

    Google Scholar 

  • Drenckhahn, D., Unsicker, K., Gröschel-Stewart, U.: Immunocytochemical demonstration of myosin and actin in peripheral nerves and the spinal cord of the rat. VIth Intern. Meeting of the Intern. Soc. Neurochem., Copenhagen, 21–26 August, 1977, in: Proc. Intern. Soc. Neurochem. 6, 115 (1977)

  • Gröschel-Stewart, U., Ceurremans, S., Lehr, I., Mahlmeister, Ch. Paar, E.: Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. II. Speciesspecific and species-non-specific antibodies to smooth and striated chicken muscle actin. Histochemistry 50, 271–279 (1977)

    Google Scholar 

  • Gröschel-Stewart, U., Schreiber, J., Mahlmeister, Ch., Weber, K.: Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. I. Antibodies to smooth and striated chicken muscle myosin. Histochemistry 46, 229–236 (1976)

    Google Scholar 

  • Isenberg, G., Rieske, E., Kreutzberg, G.W.: Distribution of actin and tubulin in neuroblastoma cells. Cytobiologie 15, 382–389 (1977)

    Google Scholar 

  • Le Beux, Y.J., Willemot, J.: An ultrastructural study of the microfilaments in rat brain by means of heavy meromyosin labeling. I. The perikaryon, the dendrites and the axon. Cell Tiss. Res. 160, 1–36 (1975)

    Google Scholar 

  • Miller, C., Kuehl, W.M.: Isolation and characterization of myosin from cloned rat glioma and mouse neuroblastoma cells. Brain Res. 108, 115–124 (1976)

    Google Scholar 

  • Nicklas, W.J., Berl, S.: Effects of cytochalasin B on uptake and release of putative transmitters by synaptosomes and on brain actomyosin-like protein. Nature 247, 471–473 (1974)

    Google Scholar 

  • Puszkin, S., Berl, S.: Actomyosin-like protein from brain. Biochim. biophys. Acta (Amst.) 256, 695–709 (1972)

    Google Scholar 

  • Puszkin, S., Berl, S., Puszkin, E., Clarke, D.D.: Actomyosin-like protein isolated from mammalian brain. Science 161, 120–171 (1968)

    Google Scholar 

  • Puszkin, S., Nicklas, W.J., Berl, S.: Actomyosin-like protein in brain: subcellular distribution. J. Neurochem. 19, 1319–1333 (1972)

    Google Scholar 

  • Thoa, N.B., Wooten, G.F., Axelrod, J., Kopin, I.J.: Inhibition of release of dopamine-β-hydroxylase and norepinephrine from sympathetic nerves by colchicine, vinblastine, or cytochalasin-B. Proc. nat. Acad. Sci. (Wash.) 69, 520–522 (1972)

    Google Scholar 

  • Unsicker, K., Drenckhahn, D., Gröschel-Stewart, U., Schumacher, U., Griesser, G.H.: Immunohistochemical evidence for myosin in peripheral nerves and spinal cord of the rat. Neuroscience (in press)

  • Wessels, N.K., Spooner, B.S., Ash, J.F., Bradley, M.O., Ludena, M.A., Taylor, E.L., Wrenn, J.T., Yamada, K.M.: Microfilaments in cellular and developmental, processes. Science 171, 135–143 (1971)

    Google Scholar 

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This work was supported by grants from Deutsche Forschungsgemeinschaft (Un 34/4, Dr 91/1, Ste 105/19). Thanks are due to Ursula König, Renate Steffens and Christine Mahlmeister for skilful technical assistance.

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Unsicker, K., Drenckhahn, D. & Gröschel-Stewart, U. Further immunofluorescence-microscopic evidence for myosin in various peripheral nerves. Cell Tissue Res. 188, 341–344 (1978). https://doi.org/10.1007/BF00222643

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