Summary
The mechanism of plasma membrane turnover was investigated using the duckling salt gland as a model system. Feeding fresh water to saltstressed ducklings results in a decrease in the Na, K-ATPase in salt gland to nonstressed levels in about 7 days, as measured by ATP hydrolysis and 3H-ouabain binding. Electron micrographs reveal that this is accompanied by a decrease in plasma membrane infoldings on the basal and lateral borders of gland secretory cells. Simultaneously there is an increase in filamentous material and a rise in acid phosphatase and peptidase activities in these cells. Cytochemistry shows that the acid phosphatase activity is mostly associated with the basal or basolateral regions of secretory cells. These observations could indicate that the removal of plasma membrane components is accomplished by internalization and digestion within the secretory cells.
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Hossler, F.E., Sarras, M.P. & Allen, E.R. Ultrastructural, cyto- and biochemical observations during turnover of plasma membrane in duck salt gland. Cell Tissue Res. 188, 299–315 (1978). https://doi.org/10.1007/BF00222639
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DOI: https://doi.org/10.1007/BF00222639