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Binding of the transition metal ion [(H20)(NH3)5Ru(II)]2+ to nucleosomal core and internucleosomal DNA

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Abstract

The goal of this study is to establish the nature of pentammineruthenium(III) binding to DNA in intact mouse liver nuclei. Also, we wish to determine whether the nucleosomal organization of mouse chromatin has a substantial effect on the relative Ru(III) binding levels of internucleosomal and nucleosomal core DNA. These questions are important because ammineruthenium compounds share chemical and biological properties with the cis-dichlorodiammineplatinum(II) or cisplatin chemotherapeutic agent. Therefore, they represent a potential class of new chemotherapeutic agents. We find that in intact nuclei the predominant DNA binding site for pentammineruthenium(II), followed by air oxidation to pentammineruthenium(III), is N-7 guanine, as is the case with cisplatin. Also, the Ru(III) distribution between internucleosomal and nucleosomal core DNA was found to be nearly identical as probed with three non-specific deoxyribonucleases.

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Authors and Affiliations

  1. Biochemistry Ph.D. Program, Department of Chemistry, University of Lowell, One University Ave., 01854, Lowell, MA, USA

    Kenneth A. Marx, Ray Kruger & Michael J. Clarke

  2. Department of Chemistry, Boston College, 02167, Chestnut Hill, MA, USA

    Kenneth A. Marx, Ray Kruger & Michael J. Clarke

Authors
  1. Kenneth A. Marx
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  2. Ray Kruger
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  3. Michael J. Clarke
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Marx, K.A., Kruger, R. & Clarke, M.J. Binding of the transition metal ion [(H20)(NH3)5Ru(II)]2+ to nucleosomal core and internucleosomal DNA. Mol Cell Biochem 86, 155–162 (1989). https://doi.org/10.1007/BF00222615

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  • DOI: https://doi.org/10.1007/BF00222615

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